Described in an advance, online publication of the journal Nature Biotechnology on February 22, 2009, the team's general method could be adapted for detecting a wide variety of compounds, including many that are relevant to diagnostic medicine and environmental work.
"This technology could be used to measure drugs and metabolites in the body or to measure toxic compounds in soil or groundwater," says Professor Gerald Joyce, M.D., Ph.D., who authored with paper with a postdoctoral fellow in his lab, Bianca Lam, Ph.D. Joyce is the dean of the faculty at Scripps Research, where he is also a professor in the Department of Molecular Biology, the Department of Chemistry, and The Skaggs Institute for Chemical Biology.
Joyce's new method is based on a class of special RNA replicator molecules that he and his colleagues reported earlier this year. In the presence of specific chemicals, these RNA molecules will replicate exponentially, detectably amplifying their concentration. And the more of the target chemical that is present, the faster the RNA molecules will replicate.
"The development of these RNA replicators provides researchers with a valuable new tool for detecting the presence of specific molecules and measuring their levels," says Richard Ikeda, Ph.D., who oversees enzymology grants at the National Institute of General Medical Sciences of the National Institute's of Health, which partially funded the research. "There is tremendous potential for application of this technology in diagnostic, environmental, and chemical testing."
Similar to DNA, RNA is a basic component of cells and plays many roles in the body, including helping to transfer genetic information from DNA into active protein enzymes, which carry out many of the body's vital functions. Scientists have known for many years that some types of RNA molecules are themselves enzymes. More than 40 years ago, Nobel laureate Francis Crick proposed that RNA, and in p
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Scripps Research Institute