Navigation Links
Scientists step up hunt for bacterial genes tied to Lyme disease
Date:10/26/2012

Investigators at The University of Texas Health Science Center at Houston (UTHealth) have accelerated the search for the bacterial genes that make the Lyme disease bacterium so invasive and persistent. The discovery could advance the diagnosis and treatment of this disease, which affects an estimated 30,000 Americans each year.

The researchers have developed a new technique that allowed them to test 15 times more bacterial genes than had been evaluated in the previous 30 years to ascertain their roles in infection. Findings appeared Oct. 25 in the journal The Public Library of Science ONE (PLOS ONE), an international, peer-reviewed, open-access, online publication.

Scientists hope to use this information to unravel the mystery of how the spiral-shaped bacterium Borrelia burgdorferi causes Lyme disease. Ticks carry the bacterium and transfer it to animals and humans when the tiny spider-like creatures bite. The Lyme disease microorganism was discovered in 1981.

"We believe that this will be one of the most significant publications in Lyme disease in the next several years. This global approach will help 'move the field forward' and also serve as a model for other pathogens with similar properties," said Steven Norris, Ph.D., the study's senior author and the vice chair for research in the Department of Pathology and Laboratory Medicine at the UTHealth Medical School.

The bacterium can invade almost any tissue in humans or animals and trigger an infection that lasts from months to years. Its symptoms include a reddish rash that often resembles a bull's eye and flu-like symptoms. The disease can lead to nervous system problems, joint inflammation and heart abnormalities. Most instances of Lyme disease can be treated with antibiotics.

"Our long-term goals are to screen, identify and characterize the virulence determinants of the Lyme disease bacterium and thereby dissect the mechanism of pathogenesis in mammals and ticks," said Tao Lin, D,V.M., the study's lead author and assistant professor of pathology and laboratory medicine at the UTHealth Medical School. "With this information, we will have a clearer picture about the virulence determinants and virulence factors for this fascinating microorganism and the mechanism of pathogenesis behind this unique, invasive, persistent pathogen."

Norris, the Robert Greer Professor of Biomedical Sciences at UTHealth, and Lin are running tests on the 1,739 genes in the bacterium to see which genes impact the microorganism's ability to spread disease.

To do this, they mutated the bacterial genes and gauged the impact in a mouse infection model. Overall, 4,479 mutated bacteria were isolated and characterized. Whereas it took researchers about three decades to knock out less than 40 bacterial genes, Norris and Lin knocked out 790 genes in a comparatively short period of time; some genes were "hit" multiple times. A newly developed screening technique, which involves signature-tagged mutagenesis and Luminex-based high-throughput screening technologies, can also be used to identify infection-related genes in other bacteria.

"This kind of study enables us to better understand the disease pathogenesis at the basic level," said Charles Ericsson, M.D., head of clinical infectious diseases at the UTHealth Medical School. "In time, such understanding of virulence properties might enable us to develop vaccine candidates, better diagnostic tools and perhaps even targeted drug intervention."


'/>"/>
Contact: Robert Cahill
Robert.Cahill@uth.tmc.edu
713-500-3030
University of Texas Health Science Center at Houston
Source:Eurekalert  

Related medicine news :

1. Scientists to study the role genes play in treating TB
2. Kessler Foundation scientists awarded New Jersey grants for brain injury research
3. Scientists Focus on Factors Behind Asthma Attacks
4. Einstein-Montefiore scientists awarded 2 NCI Provocative Questions grants
5. Leading stem cell scientists to focus on diabetes, eye diseases at Cedars-Sinai symposium
6. Diets High in Fructose May Harm Liver in Some, Scientists Warn
7. Cardiff scientists bid to develop anthrax vaccine to counteract world bioterrorism threat
8. Scripps Research Institute scientists show protein linked to hunger also implicated in alcoholism
9. Scientists Spot 5 Genes That Help Shape Faces
10. Eight scientists honored in first annual Golden Goose Awards
11. Scientists Map Genetic Blueprint of Heart
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists step up hunt for bacterial genes tied to Lyme disease
(Date:6/24/2016)... ... 2016 , ... Marcy was in a crisis. Her son James, eight, was out of control. ... and physically. , “When something upset him, he couldn’t control his emotions,” remembers Marcy. ... rocks at my other children and say he was going to kill them. If ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Global law ... magazine’s 2016 Legal Elite. The attorneys chosen by their peers for this recognition are ... , Seven Greenberg Traurig Shareholders received special honors as members of this year’s Legal ...
(Date:6/24/2016)... ... June 24, 2016 , ... Comfort Keepers® of San Diego, ... and the Road To Recovery® program to drive cancer patients to and from their ... to ensure the highest quality of life and ongoing independence. Getting to and ...
(Date:6/24/2016)... ... June 24, 2016 , ... People across the U.S. ... magazine’s Code Talker Award, an essay contest in which patients and their families pay ... be presented at the 2016 National Society of Genetic Counselors (NSGC) Annual Education Conference ...
(Date:6/24/2016)... NY (PRWEB) , ... June 24, 2016 , ... ... Haute Living, is proud to recognize Dr. Barry M. Weintraub as a prominent ... “the most beautiful women in the world, and the most handsome men, look ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... and INDIANAPOLIS , June ... receiving a Lilly Diabetes Tomorrow,s Leaders Scholarship is any ... scholarship winners, announced today online at www.diabetesscholars.org ... type 1 diabetes stand in the way of academic ... supported the Foundation,s scholarship program since 2012, and continues ...
(Date:6/23/2016)...  Guerbet announced today that it has been named ... . One of 12 suppliers to receive ... support of Premier members through exceptional local customer service ... to lower costs. ... outstanding customer service from Premier," says Massimo Carrara ...
(Date:6/23/2016)... , June 23, 2016  Experian Health, ... and transforming the patient payment and care ... innovative new products and services that will ... revenue cycle offerings. These award-winning solutions will ... workflows, remain compliant in an ever-changing environment ...
Breaking Medicine Technology: