Navigation Links
Scientists reveal DNA-enzyme interaction with first ever real time footage
Date:9/17/2007

For the first time scientists have been able to film, in real time, the nanoscale interaction of an enzyme and a DNA strand from an attacking virus. Researchers from the University of Cambridge have used a revolutionary Scanning Atomic Force Microscope in Japan to produce amazing footage of a protective enzyme unravelling the DNA of a virus trying to infect a bacterial host.

The film is available to view at: http://www.bbsrc.ac.uk/media/pressreleases/video_enzyme_unravelling_dna.html

The research, funded by the Biotechnology and Biological Sciences Research Council (BBSRC), will improve our understanding of how enzymes interact with DNA at a nanoscale but also has marked implications for scientists studying DNA repair, and could help in the search for cancer treatments.

Working with researchers in Edinburgh, Japan and India, the Cambridge team used a state-of-the-art microscope, only three of which exist in the world, and a technique known as fast-scan atomic force microscopy (AFM). Before now, scientists could only make assumptions as to how proteins and DNA interact based on indirect evidence but this new window on a fundamental biological process gives them the opportunity to view how the interaction actually occurs.

Dr Robert Henderson, who led the Cambridge research, explains: This is the first time that such a process has been seen in real time. To be able see these nano-mechanisms as they are really happening is incredibly exciting. We can actually see the enzyme threading through a loop in the viruss DNA in order to lock on to and break it, a process known as DNA cleavage.

The microscope and new techniques give us a clear view of the molecular interactions between proteins and DNA that we could only previously interpret indirectly. The indirect methods require scientists to make assumptions to interpret their data, and video footage like this can help to provide a more direct understanding of what is really happening.

Standard technology for filming on this scale can only produce one image frame every 8 minutes. However, our new work allows one frame per 500 or fewer, milliseconds.

The footage shows a bacterial type III restriction enzyme attaching itself to the DNA of a virus, in order to break the DNA before the virus has the chance to infect the bacterium. However, this could also provide a model for understanding how other enzymes and DNA, in any type of organism, including humans, interact.

This helps us understand how enzymes recognise which bit of a DNA strand to latch onto, which is important in understanding how proteins repair damaged DNA. In the long term, this could help in the search for cancer treatments, as cancer sometimes occurs where DNA is damaged but enzymes do not behave correctly in order to repair it.

Steve Visscher, interim BBSRC Chief Executive, said: BBSRC strongly supports the development of new tools and resources and this study clearly highlights the significance of cutting-edge technologies to bioscience research. It is essential that bioscientists can draw upon technologies from the physical and engineering sciences to improve their understanding of biological processes.


'/>"/>

Contact: Matt Goode
press.office@bbsrc.ac.uk
01-793-413-299
Biotechnology and Biological Sciences Research Council
Source:Eurekalert

Related medicine news :

1. Nobel Prize for Medicine shared by Three scientists
2. Scientists plan human cloning clinic in the United States
3. Scientists crack mechanism of Leptin-Obesity Hormone
4. Scientists use plant hormones to fight cancer
5. American scientists alter gene makeup of babies
6. Expose on eating disorders!! Scientists trace “brain’s eating control center pathway”
7. Scientists found ancient Human Germ Killer
8. Scientists locate key hormone involved in appetite control
9. Scientists open the book of life
10. Electronic nose by Italian scientists
11. Scientists review SARS
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... , ... June 27, 2016 , ... ... , the only authorized OSHA Training Institute Education Center headquartered in Northern California, ... their workers from extreme heat at their worksites. Employers with workers exposed ...
(Date:6/27/2016)... (PRWEB) , ... June 27, 2016 , ... "FCPX editors ... customizable inside of Final Cut Pro X," said Christina Austin - CEO of Pixel ... style. Final Cut Pro X users can now reveal the media of ...
(Date:6/27/2016)... ... , ... TherapySites, the leading website and online ... Counseling Association. This new relationship allows TherapySites to continue to extend their ... benefits and promotional offers. , "TCA is extremely excited about this new partnership, ...
(Date:6/27/2016)... ... June 27, 2016 , ... ... payment industry today announced its strategic partnership with Connance, a healthcare industry ... The two companies’ proven, proprietary technology combine to provide health systems, hospitals ...
(Date:6/27/2016)... ... June 27, 2016 , ... A revolution is underway. ... transport experience for the millions of people who require these medical transport services ... industry through the use of technology. Now, SmartEMS has put forth an industry-changing ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... 2016 Dublin ... addition of the " Global Markets for Spectroscopy ... This report focuses on the ... review, including its applications in various applications. The report ... includes three main industries: pharmaceutical and biotechnology, food and ...
(Date:6/24/2016)... June 24, 2016 Dehaier Medical Systems Ltd. ... which develops, markets and sells medical devices and wearable ... signed a strategic cooperation agreement with Hongyuan Supply Chain ... Chain") on June 20, 2016, to develop Dehaier,s new ... cooperation agreement, Dehaier will leverage Hongyuan Supply Chain,s sales ...
(Date:6/24/2016)... 24, 2016 According to a ... (Standard Pen Needles, Safety Pen Needles), Needle Length (4mm, ... Hormone), Mode of Purchase (Retail, Non-Retail) - Trends & ... studies the market for the forecast period of 2016 ... 2.81 Billion by 2021 from USD 1.65 Billion in ...
Breaking Medicine Technology: