Navigation Links
Scientists report first step in strategy for cell replacement therapy in Parkinson's disease
Date:1/24/2012

Amsterdam, NL, January 24 Induced pluripotent stem cells (iPSC) are a promising avenue for cell replacement therapy in neurologic diseases. For example, mouse and human iPSCs have been used to generate dopaminergic (DA) neurons that improve symptoms in rat Parkinson's disease models. Reporting in the current issue of the Journal of Parkinson's Disease, a group of scientists from Japan evaluated the growth, differentiation, and function of human-derived iPSC-derived neural progenitor cells (NPCs) in a primate model, elucidating their therapeutic potential.

"We developed a series of methods to induce human iPSCs to become NPCs, using a feeder-free culture method, and grafted NPCs at different stages of differentiation into the brain of a monkey PD model," explains lead investigator Jun Takahashi, MD, PhD, of Kyoto University. "We developed a method to evaluate the growth and DA activity of the grafts using magnetic resonance imaging (MRI), positron emission tomography (PET), immunocytochemistry, and behavioral analyses, all of which will be useful in preclinical research."

Investigators grafted human iPSCs into the brains of laboratory mice and a monkey treated with MPTP, a neurotoxin that causes Parkinson's symptoms. They found that iPSCs incubated in feeder-free culture generated functional midbrain DA neurons. "In previous studies, midbrain DA neurons were induced from human iPSCs, but the method required coculture with stromal mouse feeder cells or Matrigel," noted Dr. Takahashi. "Our feeder-free method would be more suitable for clinical use."

Pre-treatment with growth factors was required to promote the maturation of functional DA neurons in vivo. MRI and PET imaging allowed real-time monitoring of in vivo cell proliferation and activity. The study demonstrates that dopamine synthesis, transport, and reuptake reflect DA activity in the grafted NPCs, an approach that can also be used in human patients.

"Our results contribute to the evaluation of the survival, differentiation, and function of human iPSC-derived neuronal cells in a primate PD model. Although we have to perform additional preclinical studies using more primate models before clinical application, we believe our findings contribute as the first step for developing a strategy for cell replacement therapy in Parkinson's disease," Dr. Takahashi concludes.


'/>"/>

Contact: Daphne Watrin
d.watrin@iospress.nl
31-206-883-355
IOS Press
Source:Eurekalert

Related medicine news :

1. Gladstone scientists identify genetic mechanism linked to congenital heart disease
2. Scientists Agree to Delay Controversial Bird Flu Research
3. Scientists shed new light on link between killer cells and diabetes
4. NIH scientists identify novel approach to view inner workings of viruses
5. UNC scientists collaborate to find first major genetic mutation associated with hereditary prostate cancer risk
6. Scientists Identify Inherited Prostate Cancer Gene
7. Scientists Create First Monkeys With Mixed Genomes
8. Scientists reassess weight loss surgery for type 2 diabetes
9. Scientists crack medieval bone code
10. Scientists fixate on Ric-8 to understand trafficking of popular drug receptor targets
11. NOAA scientists: spilled oil unexpectedly lethal to fish embryos in shallow, sunlit waters
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/7/2016)... Dallas, TX (PRWEB) , ... December 07, 2016 ... ... service based in Dallas, Texas, is condemning "scam operations" carried out by unethical ... to bring these scam operations to a halt. According to Texas Premier Locksmith, ...
(Date:12/7/2016)... California (PRWEB) , ... December 07, 2016 , ... "ProBrand ... any and all media," said Christina Austin - CEO of Pixel Film Studios. , ... with easy to use drop zones. Editors can select from a variety of flip ...
(Date:12/7/2016)... , ... December 07, 2016 , ... ... and Wellness (IFW) Program at Reproductive Medicine Associates of Connecticut (RMACT). McLaughlin brings ... a team of three acupuncturists to help patients realize their family building goals. ...
(Date:12/7/2016)... ... December 07, 2016 , ... “Walking With God: Inspirational ... that will inspire the reader to be aware of God's direction in their lives. ... author, Sanford Smith, retired teacher and active church leader. , Sanford says, ...
(Date:12/7/2016)... ... December 07, 2016 , ... ... Business Architecture Innovation Summit in Reston, VA on March 21-22, 2017. This premier ... a range of experiences from a cross-section of industries such as financial services, ...
Breaking Medicine News(10 mins):
(Date:12/6/2016)... , Dec. 6, 2016  Arcturus Therapeutics, Inc. ... company, announced today that it entered into collaboration ... therapeutics for the treatment of NASH and other ... delivery platform LUNAR™ and UNA Oligomer chemistry. The ... Takeda,s long-standing commitment to and expertise in GI ...
(Date:12/6/2016)... Dec. 6, 2016  Regulus Therapeutics Inc. (NASDAQ: ... the discovery and development of innovative medicines targeting ... at its R&D day, held in ... is an anti-miR targeting microRNA-27 (miR-27) for the ... is an anti-miR targeting microRNA-17 (miR-17) for the ...
(Date:12/6/2016)... new study released today by the Alliance for Integrity ... Pricing Program will continue to expand at an unprecedented rate ... $23 billion in total purchases at the 340B price. This ... B drug reimbursement purchases over the next five years.      ... data on total drug purchases through 340B from 2005 to ...
Breaking Medicine Technology: