Navigation Links
Scientists report first step in strategy for cell replacement therapy in Parkinson's disease
Date:1/24/2012

Amsterdam, NL, January 24 Induced pluripotent stem cells (iPSC) are a promising avenue for cell replacement therapy in neurologic diseases. For example, mouse and human iPSCs have been used to generate dopaminergic (DA) neurons that improve symptoms in rat Parkinson's disease models. Reporting in the current issue of the Journal of Parkinson's Disease, a group of scientists from Japan evaluated the growth, differentiation, and function of human-derived iPSC-derived neural progenitor cells (NPCs) in a primate model, elucidating their therapeutic potential.

"We developed a series of methods to induce human iPSCs to become NPCs, using a feeder-free culture method, and grafted NPCs at different stages of differentiation into the brain of a monkey PD model," explains lead investigator Jun Takahashi, MD, PhD, of Kyoto University. "We developed a method to evaluate the growth and DA activity of the grafts using magnetic resonance imaging (MRI), positron emission tomography (PET), immunocytochemistry, and behavioral analyses, all of which will be useful in preclinical research."

Investigators grafted human iPSCs into the brains of laboratory mice and a monkey treated with MPTP, a neurotoxin that causes Parkinson's symptoms. They found that iPSCs incubated in feeder-free culture generated functional midbrain DA neurons. "In previous studies, midbrain DA neurons were induced from human iPSCs, but the method required coculture with stromal mouse feeder cells or Matrigel," noted Dr. Takahashi. "Our feeder-free method would be more suitable for clinical use."

Pre-treatment with growth factors was required to promote the maturation of functional DA neurons in vivo. MRI and PET imaging allowed real-time monitoring of in vivo cell proliferation and activity. The study demonstrates that dopamine synthesis, transport, and reuptake reflect DA activity in the grafted NPCs, an approach that can also be used in human patients.

"Our results contribute to the evaluation of the survival, differentiation, and function of human iPSC-derived neuronal cells in a primate PD model. Although we have to perform additional preclinical studies using more primate models before clinical application, we believe our findings contribute as the first step for developing a strategy for cell replacement therapy in Parkinson's disease," Dr. Takahashi concludes.


'/>"/>

Contact: Daphne Watrin
d.watrin@iospress.nl
31-206-883-355
IOS Press
Source:Eurekalert

Related medicine news :

1. Gladstone scientists identify genetic mechanism linked to congenital heart disease
2. Scientists Agree to Delay Controversial Bird Flu Research
3. Scientists shed new light on link between killer cells and diabetes
4. NIH scientists identify novel approach to view inner workings of viruses
5. UNC scientists collaborate to find first major genetic mutation associated with hereditary prostate cancer risk
6. Scientists Identify Inherited Prostate Cancer Gene
7. Scientists Create First Monkeys With Mixed Genomes
8. Scientists reassess weight loss surgery for type 2 diabetes
9. Scientists crack medieval bone code
10. Scientists fixate on Ric-8 to understand trafficking of popular drug receptor targets
11. NOAA scientists: spilled oil unexpectedly lethal to fish embryos in shallow, sunlit waters
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... ... ... “The Journey: From the Mountains to the Mission Field”: the story of a ... “The Journey: From the Mountains to the Mission Field” is the creation of published ... all ages and currently teaches a class of ladies at her church, which she ...
(Date:10/12/2017)... ... October 12, 2017 , ... Planet Fitness, one of the largest and ... plans to open a flagship location in Covington, LA at 401 N. U.S. Highway ... store next to Office Depot in the Holiday Square shopping center. Its location allows ...
(Date:10/12/2017)... ... ... Asante, a nationally recognized health system in southern Oregon, ... health joint venture through an agreement, effective October 1, 2017, to create AccentCare ... company with Asante, delivering clinically integrated care, for the past eight years. This ...
(Date:10/12/2017)... ... October 12, 2017 , ... Leading pediatric oncology experts ... Washington, D.C., for the 49th Congress of the International Society of Paediatric Oncology ... of the Center for Cancer and Blood Disorders at Children’s National, and ...
(Date:10/12/2017)... ... 2017 , ... In the United States, single-family home owners ... New York, New Jersey, Massachusetts, Texas, Virginia, Connecticut, and California—the average is $7,000 ... property-tax rates, which contributes to the relatively lower cost of living in places ...
Breaking Medicine News(10 mins):
(Date:10/2/2017)... -- Halo Labs announces the European launch of their new low ... MIBio 2017 in Cambridge, U.K on October ... biopharmaceutical samples with unprecedented speed and sensitivity while using far less ... Imaging. ... analysis system ...
(Date:9/27/2017)... CAESAREA, Israel and NEW YORK , Sept. ... company with mobile health and big data solutions, today announced that its ... today. Please check your local TV listings for when The Dr. Oz ... ... ninth season this month. ...
(Date:9/22/2017)... 2017 AVACEN Medical (AVACEN) announced that its ... helping those with the widespread pain associated with fibromyalgia ... in Essex, England commented, "I ... experiencing no sleep at all, tremendous pain, with every ... recommend [the AVACEN 100] enough, how this has and ...
Breaking Medicine Technology: