Navigation Links
Scientists report first step in strategy for cell replacement therapy in Parkinson's disease
Date:1/24/2012

Amsterdam, NL, January 24 Induced pluripotent stem cells (iPSC) are a promising avenue for cell replacement therapy in neurologic diseases. For example, mouse and human iPSCs have been used to generate dopaminergic (DA) neurons that improve symptoms in rat Parkinson's disease models. Reporting in the current issue of the Journal of Parkinson's Disease, a group of scientists from Japan evaluated the growth, differentiation, and function of human-derived iPSC-derived neural progenitor cells (NPCs) in a primate model, elucidating their therapeutic potential.

"We developed a series of methods to induce human iPSCs to become NPCs, using a feeder-free culture method, and grafted NPCs at different stages of differentiation into the brain of a monkey PD model," explains lead investigator Jun Takahashi, MD, PhD, of Kyoto University. "We developed a method to evaluate the growth and DA activity of the grafts using magnetic resonance imaging (MRI), positron emission tomography (PET), immunocytochemistry, and behavioral analyses, all of which will be useful in preclinical research."

Investigators grafted human iPSCs into the brains of laboratory mice and a monkey treated with MPTP, a neurotoxin that causes Parkinson's symptoms. They found that iPSCs incubated in feeder-free culture generated functional midbrain DA neurons. "In previous studies, midbrain DA neurons were induced from human iPSCs, but the method required coculture with stromal mouse feeder cells or Matrigel," noted Dr. Takahashi. "Our feeder-free method would be more suitable for clinical use."

Pre-treatment with growth factors was required to promote the maturation of functional DA neurons in vivo. MRI and PET imaging allowed real-time monitoring of in vivo cell proliferation and activity. The study demonstrates that dopamine synthesis, transport, and reuptake reflect DA activity in the grafted NPCs, an approach that can also be used in human patients.

<
'/>"/>
Contact: Daphne Watrin
d.watrin@iospress.nl
31-206-883-355
IOS Press
Source:Eurekalert

Page: 1 2

Related medicine news :

1. Gladstone scientists identify genetic mechanism linked to congenital heart disease
2. Scientists Agree to Delay Controversial Bird Flu Research
3. Scientists shed new light on link between killer cells and diabetes
4. NIH scientists identify novel approach to view inner workings of viruses
5. UNC scientists collaborate to find first major genetic mutation associated with hereditary prostate cancer risk
6. Scientists Identify Inherited Prostate Cancer Gene
7. Scientists Create First Monkeys With Mixed Genomes
8. Scientists reassess weight loss surgery for type 2 diabetes
9. Scientists crack medieval bone code
10. Scientists fixate on Ric-8 to understand trafficking of popular drug receptor targets
11. NOAA scientists: spilled oil unexpectedly lethal to fish embryos in shallow, sunlit waters
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/18/2014)... How did it make you feel? According to psychologists, ... such as how sad you were or how embarrassed ... you can,t stop thinking about it. , When ... of the memories, rather than how you felt, is ... negative effects of these memories, a new study suggests. ...
(Date:4/18/2014)... Diego, Calif. (April 18, 2014) ― A new study ... analyzing weekly patterns in health-related Google searches reveals a ... health strategies. , Investigators from San Diego ... and the Monday Campaigns, analyzed "healthy" Google searches (searches ... e.g., "healthy diet") originating in the U.S. from 2005 ...
(Date:4/17/2014)... of chronic inflammation in benign prostate tissue was ... this association was found even in those with ... study published in Cancer Epidemiology, Biomarkers & ... for Cancer Research. , An analysis of prostate ... placebo arm of the Prostate Cancer Prevention Trial ...
(Date:4/17/2014)... new pain relief targets that could be used ... at King,s College London made the discovery when ... periphery of the body. , Dr Marzia Malcangio ... underlying pain generation and our findings could help ... , One potential side effect of some chemotherapy ...
(Date:4/17/2014)... the April issue of Experimental Biology and ... Drs. Rex Gaskins and Paul Kenis in the ... of the University of Illinois Urbana-Champaign describe their ... reactions underlie a variety of cell functions including ... these crucial roles in regulating normal cellular behavior, ...
Breaking Medicine News(10 mins):Health News:New study suggests a better way to deal with bad memories 2Health News:New study suggests a better way to deal with bad memories 3Health News:New research shows people are thinking about their health early in the week 2Health News:Chronic inflammation may be linked to aggressive prostate cancer 2Health News:Chronic inflammation may be linked to aggressive prostate cancer 3Health News:New pain relief targets discovered 2Health News:Live cell imaging reveals distinct alterations of subcellular glutathione potentials 2
... the devastating earthquake of 8 October 2005 in northern ... risk, facing a second winter without permanent shelter.// ... around 3.5 million homeless. Estimates have shown that even ... recent landslides and flooding have left most quake survivors ...
... Scientist couple, Dr Utpal Bhadra and Monika Pal Bhadra, narrowly ... comparable to the final winners-Andrew Z. Fire and Craig C. ... (Ribonucleic acid interference) in gene silencing got the two American ... the journal Nature in 1998. ,As a matter ...
... people, suspected to be suffering from chikungunya, a viral fever ... Pune.// ,"We have collected blood samples from 10 people ... We also collected blood samples from four people in Ganjam ... Debata, the state's joint director of health told IANS Tuesday. ...
... research by scientists at the Atopy (Allergy) Research Center ... suggests that dust mites and their fecal droppings// disrupt ... to other irritants. ,Episodes of asthma and ... even in previous studies conducted by other researchers. However ...
... one of the greatest challenges facing researchers today, and recent ... a growing epidemic.// The World Health Organization has estimated that ... problems, of them nearly 300 million are obese. ... works on the source of the problem – appetite, the ...
... with breast cancer positive to hormone receptors and HER2 ... (anastrozole) from AstraZeneca to their treatment improves the length ... Phase III clinical trial sponsored by Roche showed that ... after which the cancer continued to progress. ...
Cached Medicine News:Health News:Survivors of Pakistan Earthquake Still at Risk 2Health News:Brain behind the Brawn: Vital Molecule in the Brain Controls Appetite 2
(Date:1/15/2014)... 2014  Zafgen, Inc., a leading biopharmaceutical company dedicated ... patients, today announced initial results from its Phase ... methionine aminopeptidase 2 (MetAP2), in patients with Prader-Willi ...  These results showed improvements in body weight, hunger-related ...
(Date:1/14/2014)... , Jan. 14, 2014 InformEx, North ... sellers of high-value chemistry, will hold the 30 th ... Beach Convention Center from January 21-24. ... attendees from top pharmaceutical, fine chemical and specialty chemical companies ...
(Date:1/14/2014)... LEESBURG, Va. , Jan. 14, 2014 Animal Emergency Critical ... in Leesburg, Virginia is the first in ... hyperbaric oxygen chamber. Dr. Susan M. Barnes ... years. Dr. Barnes and husband, Dr. Cole Taylor , are ...
Breaking Medicine Technology:Zafgen Announces Initial Results from Phase 2a Study of Beloranib in Patients with Prader-Willi Syndrome 2Zafgen Announces Initial Results from Phase 2a Study of Beloranib in Patients with Prader-Willi Syndrome 3Zafgen Announces Initial Results from Phase 2a Study of Beloranib in Patients with Prader-Willi Syndrome 4Media Advisory: InformEx 2014 Events & Speaker Lineup for 30th Edition Conference in Miami Beach 2Hyperbaric Veterinary Medicine (hvm) Announces Animal Emergency Critical Care Installs A Small Animal Hyperbaric Chamber 2
... Cochrane Reviews enable all those involved with ... with the very latest evidence in their ... becomes harder each year as the volume ... problem by delivering the best single source ...
This handy quick reference is a pragmatic guide to the pathophysiology, diagnosis, and management of cardiovascular disease....
The first book to translate key medical data into clear guidelines capturing the highest treatment standards for heart disease....
... ECG Notes is a quick-lookup ... This indispensable pocket guide presents the ... cardiovascular system, electrical conduction system of ... components), ACLS and CPR algorithms, emergency ...
Medicine Products: