Navigation Links
Scientists reduce protein crystal damage, improve pharmaceutical development
Date:12/18/2013

New recommendations for using X-rays promise to speed investigations aimed at understanding the structure and function of biologically important proteins information critical to the development of new drugs. Scientists from two U.S. Department of Energy national laboratories, Argonne and Brookhaven, and the University of Washington, Seattle, evaluated options to remedy problems affecting data collection in their new study.

Scientists who use powerful X-ray beams to study protein crystals face a dilemma: the beams provide the best tool for understanding a protein's structure and biological function, but they often damage the crystal, which may require repeated experiments that add time and cost to the research.

"Although X-ray crystallography is the go-to technique for determining protein structure and function, it is not without problems," said Andrzej Joachimiak at Argonne. The use of powerful X-ray beams causes radiation damage resulting in loss of data and the weak diffraction of crystals. In the end, this leads to an incomplete picture of the structure, and of how molecules interact with each other and their environment.

"The problem occurs when a protein crystal absorbs energy from incoming X-rays, which emits electrons that destroy or alter parts of the sample," Joachimiak said.

The research team examined three different X-ray-based methods for solving protein structures and recommended one called "submicrometer line focusing" as the most promising for easing the dilemma. As its name suggests, the beam strikes the protein crystal with an area smaller than a micrometer, or smaller than one thousandth of a millimeter. The tiny impact area minimizes damage. Also like its name, the beam is focused as a vertical line, delivering a more concentrated dose of X-rays per area.

The researchers also suggested using a new lens they designed that breaks the powerful beam into many mini-beams, spaced far enough apart that the damage one mini-beam creates lies outside the area probed by neighboring mini-beams.

"By carefully spacing the beams, we can lessen the damage, gather better data, and do it faster," said Joachimiak. "And because there are several beams rather than just one being released simultaneously, we can collect a greater quantity of useful data."

Synchrotron facilities like the DOE's Advanced Photon Source (APS) at Argonne contain particle accelerator systems designed to produce extraordinarily bright and high-energy X-ray beams. At these facilities, scientists can peer deeply into the atomic structure of molecules using the method of X-ray crystallography.

Scientists need to see what molecules, particularly proteins, look like. While molecules are too small to be visualized directly, their shape can be reconstructed by looking at the patterns of how X-rays diffract, or scatter, off them. Crystallography uses crystals of proteins because crystals have repeating patterns that give scientists enough data to reconstruct the exact shape as a 3-D model. From this form, scientists can often determine chemical interactions and processes that can be used to design pharmaceuticals.

In this study, the team looked at the penetration depth of the damaging electrons and at the spread or distribution of the damaged area on the protein crystal. While earlier studies recognized the issue, this team is the first to collect high-resolution data and directly measure the damaged area using a line focus beam. The team also found that earlier work had underestimated the depth of the problem.

The findings will aid synchrotron researchers as they continue to develop more brilliant and powerful instruments such as those at the APS.


'/>"/>

Contact: Jared Sagoff
jsagoff@anl.gov
630-252-5549
DOE/Argonne National Laboratory
Source:Eurekalert  

Related medicine news :

1. Scientists solving the mystery of human consciousness
2. Scientists uncover multiple faces of deadly breast cancer
3. Scientists identify major source of cells defense against oxidative stress
4. Scientists tailor cell surface targeting system to hit organelle ZIP codes
5. Scientists rewrite rulebook on breast cancer in landmark global study
6. Warwick scientists uncover how checkpoint proteins bind chromosomes
7. NIH scientists link quickly spreading gene to Asian MRSA epidemic
8. Joslin scientists identify important mechanism that affects the aging process
9. Scripps Research scientists show how memory B cells stay in class to fight different infections
10. Scientists Map Melanomas Genome
11. A*STAR scientists discover switch to boost anti-viral response to fight infectious diseases
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists reduce protein crystal damage, improve pharmaceutical development
(Date:8/18/2017)... ... , ... The Dawn Johnson Insurance Group, a Missouri-based insurance and financial planning ... support for efforts to educate the local population on cancer realities while attracting donations ... , Each day in America, roughly 4,600 new cases of cancer are diagnosed ...
(Date:8/18/2017)... The Woodlands, TX (PRWEB) , ... August 18, ... ... asset protection assistance and financial planning services to communities in east Texas, is ... the Pediatric Brain Tumor Foundation. , Founded in 1991, the Pediatric Brain Tumor ...
(Date:8/18/2017)... ... August 18, 2017 , ... Goss & Associates ... residential and commercial clients in the greater Houston region, is helping locally recognized ... the fight against cancer. , Founded by Tony and Amber Bender after losing ...
(Date:8/18/2017)... Boca Raton, Fl (PRWEB) , ... August 18, ... ... nation’s largest network of highly trained practitioners specializing in bioidentical hormone replacement therapy ... symptoms associated with hormone imbalance and conditions of aging, such as menopause, andropause, ...
(Date:8/18/2017)... IN (PRWEB) , ... August 18, 2017 , ... Inc. ... annual list, the most prestigious ranking of the nation's fastest-growing private companies. This marks ... the exclusive Inc. 5000 ranking . This year’s ranking reflects ASH’s 76 percent ...
Breaking Medicine News(10 mins):
(Date:8/7/2017)... Aug. 7, 2017 Zimmer Biomet Holdings, Inc. (NYSE ... announced that its Board of Directors has approved the payment ... quarter of 2017. The ... or about October 27, 2017 to stockholders of record as ... declarations of dividends are subject to approval of the Board ...
(Date:8/4/2017)... Md. , Aug. 4, 2017 The ... or shortly after a physician/patient consult has long been ... and was a notable focus of the largest meeting ... is according to healthcare market research firm Kalorama Information. ... care testing (POCT) offerings or related supplies and software ...
(Date:8/2/2017)... 2017   Marshall County Hospital in Benton, Kentucky has ... of hospital-acquired infections (HAIs). The new addition, Tru-D SmartUVC , short for "total room ... pathogens such as C. diff , MRSA, MERS, Ebola and more. ... Tru-D SmartUVC ... Tru-D in action in a patient room ...
Breaking Medicine Technology: