Navigation Links
Scientists generate long-sought molecular map of critical genetic machinery
Date:6/5/2014

clearly, Asturias and his colleagues began by producing highly pure quantities of a standard yeast version of Mediatorthe purification process itself being a major challenge. They then used this collection of Mediator particles to record roughly 85,000 EM images, which they categorized according to conformation. Averaging these yielded the clearest 3D model yet of the Mediator structure, to a resolution of about 18 Angstroms (1.8 billionths of a meter).

Using various other biochemical analyses, including the subtraction of different protein subunits to see how the EM images changed, the scientists were able to identify the precise locations of yeast Mediator's 25 protein subunits.

This mapping resulted in a comprehensive revision of the old rough model of Mediator's head-middle-tail structure. "After we located all the protein subunits, we realized that the head module is at the top of Mediator, not the bottom as had been thought," said Kuang-Lei Tsai, a postdoctoral fellow in the Asturias Laboratory, who was first author of the study. "These new data have helped us make sense of many previous biochemical observations."

Asturias and Tsai next collaborated with the laboratory of Joan and Ron ConawayJoan is another Kornberg alumnusat the Stowers Institute for Medical Research in Kansas City. The Conaway team had been working on human Mediator and now provided pure samples for EM imaging, as well as biochemical analyses of the subunit locations.

This work revealed that human Mediator shares the same broad architecture, implying that this structure has been, for the most part, conserved throughout the billion years of evolution that separate yeast and humans. "Basically the two Mediators have similar overall structure," said Tsai.

In the last part of the study, Asturias and Tsai used the new structural data to show how Mediator likely changes its conformation as it interacts with RNA polymerase on the one hand, and various t
'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3 4

Related medicine news :

1. Scripps Florida scientists unravel the molecular secret of short, intense workouts
2. New nanomedicine by NTU and SERI scientists to bring relief to glaucoma patients
3. Scripps Research Institute scientists win $13 million grant in AIDS vaccine effort
4. Mice with mohawks help scientists link autism to 2 biological pathways in brain
5. Temple scientists receive $11.5 million grant for heart failure research
6. TSRI scientists catch misguided DNA-repair proteins in the act
7. Scientists seek answers with space station thyroid cancer study
8. MIPT scientists develop algorithm for anti-aging remedy search
9. Scientists reveal structural secrets of enzyme used to make popular anti-cholesterol drug
10. Scientists slow brain tumor growth in mice
11. UBC scientists find new way to mobilize immune system against viruses
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists generate long-sought molecular map of critical genetic machinery
(Date:8/30/2014)... Market Research Report on Global and ... and in-depth market survey on Global and Chinese ... basic information of Cell Disruptor including its classification, ... global and China’s top manufacturers of Cell Disruptor ... market share etc. The report further analyzes quantitatively ...
(Date:8/30/2014)... Continuing to meet the skin health needs ... addition of a new doctor and office in Grand ... skin care in Grand Rapids since 1980 is merging ... doctor, my passion is making sure my patients receive ... I’m better able to focus on my patients and ...
(Date:8/30/2014)... (PRWEB) August 30, 2014 "My wife ... of a car accident," said one of two inventors ... strain in her shoulder and neck area. She tried ... reduced pain and pressure, assisting in comfort and providing ... patent-pending PILLOW CRADLE to support the head, neck and ...
(Date:8/30/2014)... Serving contented clients since inception, #1 web design ... is now offering discount for Labor Day on all ... putting forward the best of web development technology before ... of professionals and web developers and designers has expertise ... critical issues of web development and can develop websites ...
(Date:8/30/2014)... Saturday 30 August 2014: Transvenous lead extraction (TLE) ... according to data from the European Lead Extraction ConTRolled ... ESC Congress 2014 by Dr Maria Grazia Bongiorni, chair ... "The number of cardiac implantable electronic devices (CIED) implant ... technology the number of complications has increased and leads ...
Breaking Medicine News(10 mins):Health News:Cell Disruptor Industry Global and Chinese Analysis for 2009-2019 in New Research Report at ReportsnReports.com 2Health News:Cell Disruptor Industry Global and Chinese Analysis for 2009-2019 in New Research Report at ReportsnReports.com 3Health News:Cell Disruptor Industry Global and Chinese Analysis for 2009-2019 in New Research Report at ReportsnReports.com 4Health News:Expanding Care at the Forefront of Dermatology 2Health News:Profit By Outsourcing Offering Discount for Labor Day on all Web Development Services 2Health News:Transvenous lead extraction clinically successful in 98 percent of cases 2Health News:Transvenous lead extraction clinically successful in 98 percent of cases 3
... Biotech Group (USA), Inc.,(the "Company" or "Tiens", NYSE AlternextUS: ... for the twelve months ended,December 31, 2008. , ... 31, 2008, revenue was $77.2 million,an increase of 40.7% ... 31, 2007. , Net income ...
... John Lawson Cullison, Jr., an artist known for his work ... lost his ability to paint due to the effects of ... in cartilage tissue and symptoms include: pain, tenderness in the ... Pain management and joint replacement surgery are the typical ...
... for 21st century patient safety, says Dr. FriedbergCORONA ... Society of Anesthesiologists, (ASA) April ,Anesthesiology, reports of ... over medication (translation: ,TOO MUCH, anesthesia)," says Dr. ... brain activity monitors (BAMs), FDA approved and available ...
... new study in The Journal of Nursing Administration ... patients when continuous vigilance monitoring is used on medical-surgical ... Patrick Sullivan, President and CEO of Hoana Medical, Inc. ... RNs about potential patient deterioration so they can respond ...
... 31 , - ... and Smartphone,Applications , Elsevier, ... the winners in the Elsevier Article 2.0 Contest ... creative and useful solutions for rendering journal articles ...
... across North Carolina , ... Englewood, CO (Vocus) March ... science careers and information, unveiled the seventh edition of the ... ,This regional campaign is a promotional effort highlighting biotechnological, pharmaceutical and ...
Cached Medicine News:Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 2Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 3Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 4Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 5Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 6Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 7Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 8Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 9Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 10Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 11Health News:Tiens Biotech Group (USA) Reports Record Twelve-Month Results 12Health News:Korean Stem Cells Allow Artist to Paint Again 2Health News:American Society of Anesthesiologists - 2,211 Patient Deaths From OVER Medication 2Health News:Continuous Vigilance Monitoring Technology Benefits Nurses & Patients 2Health News:Three Prize Winners Awarded in Elsevier's Article 2.0 Contest 2Health News:Three Prize Winners Awarded in Elsevier's Article 2.0 Contest 3Health News:BioSpace Launches the 2009 Bio NC™ Hotbed Campaign 2
(Date:8/29/2014)... 2014 /PRNewswire-iReach/ -- Market Research Report on Global ... a professional and in-depth market survey on ... The report firstly reviews the basic information of ... manufacturing technology. The report then explores global and ... their product specification, capacity, Production value, and market ...
(Date:8/29/2014)... 29, 2014 Pomerantz LLP has filed a ... "Company") (NASDAQ: EDAP ) and certain of ... District Court, Southern District of New York ... of a class consisting of all persons or entities ... July 30, 2014, inclusive (the "Class Period"). This class action ...
(Date:8/29/2014)... Aug. 29, 2014  BC Technical, the nation,s leading ... Medical Imaging, a leader in sales, service and installation ... allows BC Technical to continue to expand their resources ... and CT modalities. "We are committed ... Mark Alvarez , president and CEO of BC Technical. ...
Breaking Medicine Technology:X-Ray Crystallography Scanner Industry For Global and Chinese Market Forecast to 2019 2X-Ray Crystallography Scanner Industry For Global and Chinese Market Forecast to 2019 3X-Ray Crystallography Scanner Industry For Global and Chinese Market Forecast to 2019 4X-Ray Crystallography Scanner Industry For Global and Chinese Market Forecast to 2019 5SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against EDAP TMS S.A. and Certain Officers - EDAP 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against EDAP TMS S.A. and Certain Officers - EDAP 3BC Technical Acquires Polaris Medical Imaging 2
... 2012   CareFusion today announced an agreement to ... that helps hospital pharmacies better manage inventory, reduce pharmaceutical ... not disclosed. The acquisition will advance CareFusion,s ... to manage medication throughout their facilities – from ...
... March 6, 2012 Life Length, the ... Drs Sandy Chang and Kathleen Collins have joined its ... CEO, Life Length, noted, "We are delighted to welcome ... SAB. They will help advance our global leadership.  Dr ...
Cached Medicine Technology:CareFusion To Acquire Pharmacy Automation Technology Leader 2CareFusion To Acquire Pharmacy Automation Technology Leader 3CareFusion To Acquire Pharmacy Automation Technology Leader 4Drs Sandy Chang and Kathleen Collins Join LIFE LENGTH®'s Scientific Advisory Board 2
... ARUP Laboratories is a national reference laboratory ... research and development. ARUP offers an extensive ... medical tests in clinical and anatomic pathology. ... Laboratories' clients include more than half of ...
... ARUP Laboratories is a national reference ... laboratory research and development. ARUP offers an ... unique medical tests in clinical and anatomic ... ARUP Laboratories' clients include more than half ...
... Laboratories is a national reference laboratory and ... and development. ARUP offers an extensive test ... tests in clinical and anatomic pathology. Owned ... clients include more than half of the ...
... national reference laboratory and a worldwide leader ... offers an extensive test menu of highly ... and anatomic pathology. Owned by the University ... than half of the nation's university teaching ...
Medicine Products: