Navigation Links
Scientists find donut-shaped structure of enzyme involved in energy metabolism
Date:2/16/2010

COLUMBIA, Mo. If subway terminals didn't exist and people had to exit subway stations to switch subway lines, transit time would increase. People also may encounter distractions, such as grabbing a cup of coffee, instead of getting on the other line. Molecules also use "terminals" to save transit time during enzyme-catalyzed processes. Using advanced X-radiation techniques, University of Missouri researchers were able to visualize one of these terminals inside of an enzyme that degrades proline, which is an amino acid that has a central role in metabolism. In humans, proline is important for suppression of cancer, cell death and oxidation. Understanding the structure of this enzyme will help scientists better understand how it functions and develop drugs that may inhibit its catalytic function.

"This is an aesthetically interesting enzyme that resembles a donut-shaped ring," said John Tanner, professor in the Department of Chemistry and the Department of Biochemistry. "Hidden under the surface of the protein is a system of tunnels and rooms - like a subway system for molecules. The purpose of this system is to provide an interior passageway connecting the two catalytic sites of the enzyme. The movement of reactant molecules through this passageway is known as channeling, which makes enzymes efficient by isolating the reactants from other enzymatic reactions. Channeling potentially allows for decreased transit time between catalytic sites and protection from competing enzymatic reactions. The reactions occur without the reactants ever leaving the confines of the protein, which is efficient."

In the study, several proline-degrading proteins were screened for their ability to crystallize. A crystal is needed in order to perform X-ray diffraction experiments, which provide high resolution images of the protein's three-dimensional structure. Additional studies using small-angle X-ray scattering and centrifugation provided crucial information about the protein's donut shape. These techniques help researchers determine the structure and composition of the enzyme.

"The complementary methods of the X-ray crystallography, small-angle X-ray scattering, and centrifugation gave us a whole picture of the structure of the enzyme," Tanner said. "Knowing the structure of the enzyme helps us understand the function of the enzyme. Once we know an enzyme's structure, we can begin to interpret other important data, such as the enzyme's role in specific reactions, how its activity is controlled and how a drug could inhibit the enzyme."

The study, "Crystal structure of the bifunctional Proline utilization A flavoenzyme from Bradyrhizobium japonicum," was published in Proceedings of the National Academy of Sciences this month.


'/>"/>

Contact: Kelsey Jackson
JacksonKN@missouri.edu
573-882-8353
University of Missouri-Columbia
Source:Eurekalert  

Related medicine news :

1. New prion protein discovered by Canadian scientists may offer insight into mad cow disease
2. Scientists Probe Sepsis Deadly Secrets
3. Scientists puzzled by severe allergic reaction to cancer drug in the middle Southern US
4. Scientists Develop Natural Protection for Stored Foods
5. Scientists detect presence of marburg virus in african fruit bats
6. Scientists Spot Brains Free Will Center
7. Scientists ID Likely Culprit in Popcorn Lung
8. Scientists explain how insulin secreting cells maintain their glucose sensitivity
9. Scripps Research scientists shed new light on how antibodies fight HIV
10. Scientists, physicians present latest findings in personalized cancer treatment and prevention
11. Scientists demonstate link between genetic variant and effectiveness of smoking cessation meds
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists find donut-shaped structure of enzyme involved in energy metabolism
(Date:5/31/2016)... ... May 31, 2016 , ... Fire Door Solutions, ... joined the Fire Door Solutions’ team. Keith will serve Fire Door Solutions ... in-field re-labeling. He will be instrumental in developing educational curriculum and leading ...
(Date:5/31/2016)... ... 31, 2016 , ... More than 80 representatives of the Hepatitis B ... Cancer Foundation held an event on National Hepatitis Testing Day outside of Philadelphia City ... the leading cause of liver cancer. , Foundation leaders and the citywide coalition they ...
(Date:5/31/2016)... TEXAS (PRWEB) , ... May 31, 2016 , ... ... live in Greater Houston Healthconnect’s (Healthconnect) regional health information exchange, which enables physicians ... medical records for their patients from other participating organizations in the exchange. SJMC’s ...
(Date:5/31/2016)... ... May 31, 2016 , ... When ... he knew it was something that contractors should have at their disposal on ... gets,” says Butch, CertainTeed’s Director of Contractor Programs. , As a result, ...
(Date:5/31/2016)... ... May 31, 2016 , ... Spartan Bioscience today introduced ... portability and convenience. , The Cube is exceptionally small—it takes up the space ... fits easily into any space, whether in a hospital, doctor’s office, or pharmacy. ...
Breaking Medicine News(10 mins):
(Date:5/27/2016)... 27, 2016 According to the ... is driving ambulatory blood pressure monitoring system market growth. ... their ability to respond to different pressure rates, leading ... lead to various cardiovascular disorders such as heart failure, ... diseases are growing in prevalence each year. WHO estimates ...
(Date:5/27/2016)... -- Amarantus BioScience Holdings, Inc. (OTCQX: ... Medicine, Neurology and Orphan Diseases, today announced that President & CEO ... investor conferences: SeeThru Equity MicroCap Conference   ... York City , NY When: Tuesday, May 31 ...   Where: Grand Hyatt Hotel, 109 East 42 nd ...
(Date:5/26/2016)... 2016 A key trend that ... emergence of new treatments. Cardax, a development stage life ... The therapy is expected to fulfil large unmet medical ... conducting studies to develop new treatments for osteoarthritis. One ... involved in osteoarthritis are being investigated, and early trials ...
Breaking Medicine Technology: