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Scientists Make Clones of Mice Dead 16 Years
Date:11/3/2008

ramming at the Center for Developmental Biology in Kobe, Japan, and his colleagues took brain tissue from a mouse strain frozen at minus 20 degrees Celsius for up to 16 years and transferred the nuclei (containing the genetic material) to empty egg cells.

These two-cell embryos were used to generate embryonic stem cell lines that resulted in 12 healthy cloned mice, which grew into normal adults.

The technique did not require nuclei from an intact donor cell. The cloning efficiency was about the same as using conventional cloning methods, the study authors stated.

The findings were published in the Nov. 3-7 issue of Proceedings of the National Academy of Sciences.

More information

The U.S. National Institutes of Health has more on stem cells.



SOURCES: Teruhiko Wakayama, Ph.D., Laboratory for Genomic Reprogramming, Center for Developmental Biology, Kobe, Japan; Richard Finnell, Ph.D., professor, Center for Environmental and Genetic Medicine, Texas A&M Health Science Center Institute of Biosciences and Technology, and director, Texas Institute for Genomic Medicine, both in Houston; Nov. 3-7, 2008, Proceedings of the National Academy of Sciences


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