Navigation Links
Rice developing mobile DNA test for HIV
Date:6/5/2014

Rice University bioengineers are developing a simple, highly accurate test to detect signs of HIV and its progress in patients in resource-poor settings.

The current gold standard to diagnose HIV in infants and to monitor viral load depends on lab equipment and technical expertise generally available only in clinics, said Rice bioengineer Rebecca Richards-Kortum. The new research features a nucleic acid-based test that can be performed at the site of care.

Richards-Kortum, director of the Rice 360˚: Institute for Global Health Technologies, and her colleagues reported their results in the American Chemical Society journal Analytical Chemistry.

The proof-of-concept work by co-lead authors Zachary Crannell and Brittany Rohrman, both graduate students in the Richards-Kortum lab, follows their similar technique to detect the parasite that causes the diarrheal disease cryptosporidiosis, reported earlier this year.

The new technique would replace a complex lab procedure based on polymerase chain reaction with one that relies on recombinase polymerase amplification (RPA), a method to quickly amplify that is, multiply genetic markers found in blood to levels where they can be easily counted. In a test the team calls qRPA, a specific sequence in HIV DNA is targeted and tagged with fluorescent probes that can be seen and quantified by a portable machine. Software analysis of the fluorescing DNA allows clinicians to determine with great accuracy whether the virus is present in a patient's blood and/or how much is there.

The researchers calibrated the test by also amplifying an internal positive control not found in human blood. "It's amplified by the same primers as the HIV sequence, so it tells us that the assay is working properly," Rohrman said.

The students originally intended their work to look for HIV in infants, but the technique can also help to track viral loads in older patients. "It's important for clinicians to be able to quantitatively monitor patients' viral loads in order to ensure the disease is responding to therapy," Crannell said.

To be clinically viable, a DNA-based test for HIV has to be able to quantify virus loads over four orders of magnitude, from very low to very high, the researchers said. They reported the Rice test easily meets that goal.

They are developing tools for low-resource settings where high-tech lab equipment is not available. Although they used a thermal cycler, the researchers are working on a technique that will keep the entire procedure between room and body temperatures so that it can be performed at the point of care in the developing world.

The research was funded by a grant from the Bill & Melinda Gates Foundation through the Grand Challenges in Global Health Initiative.


'/>"/>

Contact: David Ruth
david@rice.edu
713-348-6327
Rice University
Source:Eurekalert  

Related medicine news :

1. Study: Women not getting enough exercise; at risk of developing metabolic syndrome
2. Protein prevents DNA damage in the developing brain and might serve as a tumor suppressor
3. IU Simon Cancer Center joins WellPoint in developing health care solutions
4. Surprising results for use of dialysis for kidney failure in developing world
5. Pediatric tumors traced to stem cells in developing brain​​
6. Researchers developing new multiple sclerosis drug that can be taken orally
7. Researchers developing bioadhesive gel to protect women from HIV and HSV infections
8. Undergrads invent cell phone screener to combat anemia in developing world
9. LA BioMed investigator, Dr. Yutaka Niihara, developing novel cellular therapy
10. Global sleeplessness epidemic affects an estimated 150 million in developing world
11. By studying animal health, researchers find improved ways for developing, testing cancer therapies
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Rice developing mobile DNA test for HIV
(Date:7/24/2017)... ... July 24, 2017 , ... Cheerag D. Upadhyaya , M.D., M.Sc., FAANS ... Institute (SLMBNI), part of Saint Luke’s Health System . Dr. Upadhyaya has ... Upadhyaya, MD. M.Sc., FAANS joins Stanley P. Fisher, M.D., who has served ...
(Date:7/24/2017)... ... 24, 2017 , ... BayMark Health Services has been awarded ... (SAMHSA) State Targeted Response to the Opioid Crisis program. The funding, awarded ... development of a hub and spoke model for opioid treatment in California. The ...
(Date:7/24/2017)... ... 2017 , ... Peruvian Ayahuasca retreat, East West Lodge, announced ... and divided between two full-time university students enrolled in U.S. or Canadian accredited ... apply for the scholarship, students are asked to submit an essay between 750 ...
(Date:7/24/2017)... ... 2017 , ... Axiad IDS , a leading provider of trusted identities ... WALLIX to expand its solution to help government contractors more quickly and cost-effectively ... ways to address the authentication requirements within NIST SP800-171, but no matter how ...
(Date:7/24/2017)... ... July 24, 2017 , ... A CDC study shows that, although people are taking more ... 18 to 29), had at least one case of sunburn within the past year. It’s ... people to ignore the issue. However, only recently have people become conscientious of the risks ...
Breaking Medicine News(10 mins):
(Date:7/21/2017)... Md. and RESEARCH TRIANGLE PARK, ... Therapeutics Corporation (NASDAQ: UTHR ) announced today ... results before the market opens on Thursday, July 27, ... will host a teleconference on Thursday, July 27, 2017, ... by dialing 1-877-351-5881, with international callers dialing 1-970-315-0533.  A ...
(Date:7/17/2017)... Fla. , July 17, 2017  MedX Holdings, ... branded medical testing, strengthening and rehabilitation equipment, today announced ... Machine Program. MedX is considered the gold standard for ... leader in specialized medical strengthening equipment. ... a lease with the physician or practice who prescribe ...
(Date:7/13/2017)... July 13, 2017 RK Logistics Group, Inc. was ... certification for its Fremont, CA headquarters ... the Tri-Valley and San Jose for ... of Fremont , with its Fremont Innovation District, is ... such a powerful resource to the hundreds of biotech, pharma ...
Breaking Medicine Technology: