Navigation Links
Rice University opens new window on Parkinson's disease
Date:12/17/2012

HOUSTON (Dec. 17, 2012) Rice University scientists have discovered a new way to look inside living cells and see the insoluble fibrillar deposits associated with Parkinson's disease.

The combined talents of two Rice laboratories one that studies the misfolded proteins that cause neurodegenerative diseases and another that specializes in photoluminescent probes led to the spectroscopic technique that could become a valuable tool for scientists and pharmaceutical companies.

The research by the Rice labs of Angel Mart and LauraSegatori appeared online this month in the Journal of the American Chemical Society.

The researchers designed a molecular probe based on the metallic element ruthenium. Testing inside live neuroglioma cells, they found the probe binds with the misfolded alpha-synuclein proteins that clump together and form fibrils and disrupt the cell's functions. The ruthenium complex lit up when triggered by a laser but only when attached to the fibril, which allowed aggregation to be tracked using photoluminescence spectroscopy.

Researchers trying to understand molecular mechanisms of protein misfolding have had limited alternatives to monitor protein aggregation in cells, Mart said. A probe that can monitor the formation of aggregates should be of great value in the search for drugs that break up fibrils or prevent them from ever forming.

Two years ago, Mart, an assistant professor of chemistry and bioengineering, and Rice graduate student Nathan Cook revealed their metallic compounds that switch on like a light bulb when they attach to misfolded proteins; that study involved the beta amyloids that form plaques in the brains of Alzheimer's sufferers.

At about the same time, Cook approached Segatori, the T.N. Law Assistant Professor of Chemical and Biomolecular Engineering and assistant professor of biochemistry and cell biology, to ask if she would serve on his dissertation committee. They started talking about his work with Mart, and Segatori quickly saw the potential of a partnership.

Segatori has made important strides in the study of diseases caused by proteins that misfold and clump together. Alzheimer's, Parkinson's and Gaucher diseases are examples, all the result of genetic mutations or conditions that disrupt the way proteins fold and keep them from performing their functions.

"There are a few compounds you can use to detect the presence of these types of protein aggregates, but none of them have been reported to work in cells," Segatori said. "When you're thinking about developing a therapeutic strategy, you want to be able to detect the presence of fibril aggregates in living cells, or even in animals. It's been very nice to collaborate with someone with the expertise to do this."

"The connection between Parkinson's and Alzheimer's is natural, although they are very different diseases because Alzheimer's beta amyloid peptides are extracellular, while the onset of Parkinson's is associated with alpha-synuclein protein inside cells," Mart said. "We always thought we could apply the same techniques we used for beta amyloids to probe the aggregation of other proteins.

"When we learned that Laura has cells that overexpress alpha-synuclein, we thought, 'That's perfect.' She had the system and we had the probes," he said.

Segatori pointed out the ruthenium complex has no therapeutic benefit for Parkinson's sufferers, but "is a step toward understanding the chemistry, which obviously will help in the development of drugs."

They see the possibility that metallic complexes can be tailored to tag aggregates implicated in other degenerative diseases. "Metal complexes are like Legos, in the sense that you can attach whatever you want to them," Cook said.

As a proof of principle, the researchers created an in vitro cell model of Parkinson's disease and found their ruthenium complexes clearly labeled fibrillar alpha-synuclein proteins in cells.

"We can use it to test our strategies to prevent misfolding of proteins or to increase their degradation, so they will be eliminated," Segatori said. "It will be an easy tool to use for a lot of experiments."


'/>"/>
Contact: David Ruth
david@rice.edu
713-348-6327
Rice University
Source:Eurekalert  

Related medicine news :

1. Women’s Excellence in Bladder Control Partners with University Compounding Pharmacy in Troy, MI to Help Women with Chronic Pelvic Pain Conditions
2. Center For Sight Expands Services in University Park; David Hunt, O.D., Becomes Part of Center For Sight’s Comprehensive Eye Care Team
3. Naturecast Pets revisits New Study from University of Adelaide that may Help Dogs with Arthritis
4. University at Buffalo, The State University of New York (SUNY) Goes Live with Osprey’s Conflict of Interest COI RiskManager™
5. University of Southern Mississippi Professor Collaborates with Houston Police for Eyewitness Research Study
6. Western University researchers make breakthrough in arthritis research
7. DFG funds new Collaborative Research Center at the Mainz University Medical Center
8. University of Cincinnati leads first trial on steroid and CNI withdrawal post-transplant
9. Thomas Jefferson University honoring Axel Ullrich with Lennox K. Black International Prize
10. University Hospitals & Philips Healthcare to showcase imaging technology at Cleveland Medical Mart
11. University of Tennessee study: Unexpected microbes fighting harmful greenhouse gas
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Rice University opens new window on Parkinson's disease
(Date:10/13/2017)... ... October 13, 2017 , ... Dickinson Insurance and ... and financial preparation services, is providing an update on a charitable event that ... City Rescue is a locally recognized nonprofit that provides shelter and care for ...
(Date:10/13/2017)... Nevada (PRWEB) , ... October 13, 2017 , ... ... Hemp CBD Oil utilizing Purzorb™ technology. Applying the Purzorb™process to full spectrum CBD oil ... required and providing a CBD form that can be easily incorporated into liquid products, ...
(Date:10/13/2017)... ... October 13, 2017 , ... The Visiting Nurse Association (VNA) of ... collection of specialty vendors and unique items from across the nation, this holiday-themed event ... wellness services offered by the VNA. The boutique will be open Saturday, November ...
(Date:10/13/2017)... ... October 13, 2017 , ... Coveros, ... Development, has been awarded a contract by the Center for Medicare and Medicaid ... to accelerate the enterprise use of Agile methodologies in a consistent and high ...
(Date:10/12/2017)... ... ... Planet Fitness, one of the largest and fastest growing franchisors and operators ... location in Covington, LA at 401 N. U.S. Highway 190, in January of 2018. ... in the Holiday Square shopping center. Its location allows it to serve both Covington ...
Breaking Medicine News(10 mins):
(Date:10/12/2017)... SAN DIEGO , Oct. 12, 2017 ... has recognized the company with their  2017 New Product Innovation ... is based on extensive primary and secondary medical device market ... AVACEN Medical, through its first-to-market OTC, drug-free pain relief product, ... a unique approach to treating fibromyalgia widespread pain. ...
(Date:10/11/2017)... 11, 2017  Caris Life Sciences ® , a ... promise of precision medicine, today announced that St. Jude ... Oncology Alliance™ (POA) as its 17 th member. ... Jude Crosson Cancer Institute will help develop standards of ... tumor profiling, making cancer treatment more precise and effective. ...
(Date:10/10/2017)... , Oct. 10, 2017   West Pharmaceutical ... innovative solutions for injectable drug administration, today shared the ... ID Adapter for improving the intradermal administration of polio ... Skin Vaccination Summit in May 2017 by Dr. ... Polio Department, World Health Organization (WHO), and recently published ...
Breaking Medicine Technology: