Navigation Links
Researchers zero in on protein that destroys HIV
Date:8/24/2010

Using a $225,000 microscope, researchers have identified the key components of a protein called TRIM5α that destroys HIV in rhesus monkeys.

The finding could lead to new TRIM5α-based treatments that would knock out HIV in humans, said senior researcher Edward M. Campbell, PhD, of Loyola University Health System.

Campbell and colleagues report their findings in an article featured on the cover of the Sept. 15, 2010 issue of the journal Virology, now available online.

In 2004, other researchers reported that TRIM5α protects rhesus monkeys from HIV. The TRIM5α protein first latches on to a HIV virus, then other TRIM5α proteins gang up and destroy the virus.

Humans also have TRIM5α, but while the human version of TRIM5α protects against some viruses, it does not protect against HIV.

Researchers hope to turn TRIM5α into an effective therapeutic agent. But first they need to identify the components in TRIM5α that enable the protein to destroy viruses. "Scientists have been trying to develop antiviral therapies for only about 75 years," Campbell said. "Evolution has been playing this game for millions of years, and it has identified a point of intervention that we still know very little about."

TRIM5α consists of nearly 500 amino acid subunits. Loyola researchers have identified six 6 individual amino acids, located in a previously little-studied region of the TRIM5α protein, that are critical in the ability of the protein to inhibit viral infection. When these amino acids were altered in human cells, TRIM5α lost its ability to block HIV-1 infection. (The research was done on cell cultures; no rhesus monkeys were used in the study.)

By continuing to narrow their search, researchers hope to identify an amino acid, or combination of amino acids, that enable TRIM5α to destroy HIV. Once these critical amino acids are identified, it might be possible to genetically engineer TRIM5α to make it more effective in humans. Moreover, a better understanding of the underlying mechanism of action might enable the development of drugs that mimic TRIM5α action, Campbell said.

In their research, scientists used Loyola's wide-field "deconvolution" microscope to observe how the amino acids they identified altered the behavior of TRIM5α. They attached fluorescent proteins to TRIM5α to, in effect, make it glow. In current studies, researchers are fluorescently labeling individual HIV viruses and measuring the microscopic interactions between HIV and TRIM5α.

"The motto of our lab is one of Yogi Berra's sayings -- 'You can see a lot just by looking,'" Campbell said.


'/>"/>

Contact: Jim Ritter
jritter@lumc.edu
708-216-2445
Loyola University Health System
Source:Eurekalert

Related medicine news :

1. Researchers map all the fragile sites of the yeast Saccharomyces cerevisiaes genome
2. UH Case Medical Center researchers publish promising findings for advanced cervical cancer
3. Researchers discover new way to kill pediatric brain tumors
4. Researchers Who Discovered First Genes for Stuttering will Present Findings to the National Stuttering Association
5. Researchers create drug to keep tumor growth switched off
6. Urine protein test might help diagnose kidney damage from lupus, UT Southwestern researchers find
7. GUMC researchers say flower power may reduce resistance to breast cancer drug tamoxifen
8. Clemson researchers develop hands-free texting application
9. Researchers find biomarkers in saliva for detection of early-stage pancreatic cancer
10. Researchers chart genomic map spanning over 2 dozen cancers
11. Researchers discover second protective role for tumor-suppressor
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/19/2017)... ... February 19, 2017 , ... ProBrand Glitch is a set of 30 glitch distortion logo reveals ... easily reveal any logo with a glitch reveal in just a few clicks of a ... and animation of each preset. Use these presets to add a uniquely animated logo to ...
(Date:2/19/2017)... (PRWEB) , ... February 19, 2017 , ... ... solutions for connected home healthcare, will join forces with Healthwise ® at ... education. Healthwise, the industry leader in evidence-based health education, technology and services, ...
(Date:2/18/2017)... Waltham, Massachusetts (PRWEB) , ... February 17, 2017 ... ... be the leading source of disruptive innovation in the industry, according to the ... Insights Reports are based on surveys of the NEJM Catalyst Insights Council, a ...
(Date:2/18/2017)... ... February 17, 2017 , ... CONTACT:, Glenn Vallecillos, MD, FACS, ... surgeon Dr. Glenn Vallecillos experiments SculpSure, the hot, new, aesthetic, non-surgical ... has been centered around that idea that to achieve ones desired results a ...
(Date:2/18/2017)... ... February 18, 2017 , ... ProParagraph Fashion Volume 2 features ... ProParagraph Fashion Volume 2 for all multi-line FCPX project needs. Great for ... and choose from hand-crafted trend-setting designs with smooth animations that will add stylistic ...
Breaking Medicine News(10 mins):
(Date:2/16/2017)... , Feb. 16, 2017  AcelRx Pharmaceuticals, Inc. ... company focused on the development and commercialization of ... pain, announced that Vincent J. Angotti ... member of the company,s board of directors, effective ... two decades of experience leading executive and commercial ...
(Date:2/16/2017)... and SCOTTSDALE, Ariz. , Feb. 16, 2017 ... Information Exchange (HIE), announced today it is partnering with Orion ... HIE contains 98% of all Maine ... Maine hospitals and over 500 ambulatory care sites. ... HealthInfoNet plans to deploy Orion Health,s Amadeus , a ...
(Date:2/16/2017)... -- Die Stärkung der regulatorischen Infrastruktur ... Medizinproduktebranche oberste Priorität. Während die UDI-Implementierung in Nordamerika ... unmittelbar anstehende regulatorische Änderungen auf dem europäischen Markt ... ... Aufsichtsbehörden, Hersteller von Medizintechnik, Gesundheitsversorger und ...
Breaking Medicine Technology: