Navigation Links
Researchers zero in on protein that destroys HIV

Using a $225,000 microscope, researchers have identified the key components of a protein called TRIM5α that destroys HIV in rhesus monkeys.

The finding could lead to new TRIM5α-based treatments that would knock out HIV in humans, said senior researcher Edward M. Campbell, PhD, of Loyola University Health System.

Campbell and colleagues report their findings in an article featured on the cover of the Sept. 15, 2010 issue of the journal Virology, now available online.

In 2004, other researchers reported that TRIM5α protects rhesus monkeys from HIV. The TRIM5α protein first latches on to a HIV virus, then other TRIM5α proteins gang up and destroy the virus.

Humans also have TRIM5α, but while the human version of TRIM5α protects against some viruses, it does not protect against HIV.

Researchers hope to turn TRIM5α into an effective therapeutic agent. But first they need to identify the components in TRIM5α that enable the protein to destroy viruses. "Scientists have been trying to develop antiviral therapies for only about 75 years," Campbell said. "Evolution has been playing this game for millions of years, and it has identified a point of intervention that we still know very little about."

TRIM5α consists of nearly 500 amino acid subunits. Loyola researchers have identified six 6 individual amino acids, located in a previously little-studied region of the TRIM5α protein, that are critical in the ability of the protein to inhibit viral infection. When these amino acids were altered in human cells, TRIM5α lost its ability to block HIV-1 infection. (The research was done on cell cultures; no rhesus monkeys were used in the study.)

By continuing to narrow their search, researchers hope to identify an amino acid, or combination of amino acids, that enable TRIM5α to destroy HIV. Once these critical amino acids are identified, it might be possible to genetically engineer TRIM5α to make it more effective in humans. Moreover, a better understanding of the underlying mechanism of action might enable the development of drugs that mimic TRIM5α action, Campbell said.

In their research, scientists used Loyola's wide-field "deconvolution" microscope to observe how the amino acids they identified altered the behavior of TRIM5α. They attached fluorescent proteins to TRIM5α to, in effect, make it glow. In current studies, researchers are fluorescently labeling individual HIV viruses and measuring the microscopic interactions between HIV and TRIM5α.

"The motto of our lab is one of Yogi Berra's sayings -- 'You can see a lot just by looking,'" Campbell said.


Contact: Jim Ritter
Loyola University Health System

Related medicine news :

1. Researchers map all the fragile sites of the yeast Saccharomyces cerevisiaes genome
2. UH Case Medical Center researchers publish promising findings for advanced cervical cancer
3. Researchers discover new way to kill pediatric brain tumors
4. Researchers Who Discovered First Genes for Stuttering will Present Findings to the National Stuttering Association
5. Researchers create drug to keep tumor growth switched off
6. Urine protein test might help diagnose kidney damage from lupus, UT Southwestern researchers find
7. GUMC researchers say flower power may reduce resistance to breast cancer drug tamoxifen
8. Clemson researchers develop hands-free texting application
9. Researchers find biomarkers in saliva for detection of early-stage pancreatic cancer
10. Researchers chart genomic map spanning over 2 dozen cancers
11. Researchers discover second protective role for tumor-suppressor
Post Your Comments:
(Date:11/24/2015)... ... November 24, 2015 , ... Cancer patients, ... and experiences at a live taping of the next CURE Connections® ... of Gastrointestinal Cancers 2015 Symposium at Georgetown University Hotel & Conference Center in ...
(Date:11/24/2015)... ... 2015 , ... With Thanksgiving right around the corner, holiday travel season is ... your family and vehicle. , According to the National Highway Traffic Safety Administration, 301 ... sharing the following safety tips from the NHTSA: , ...
(Date:11/24/2015)... ... November 24, 2015 , ... Chiropractic student Katie Benson received ... in Overland Park, Kansas. Benson, a fifth-trimester student in the university’s College of ... on October 16. , “Katie is very excited and greatly appreciative to receive ...
(Date:11/24/2015)... ... November 24, 2015 , ... Bibliomotion is thrilled to announce ... about Reinvention and Diversity by Nancy M. Schlichting, Chief Executive Officer of ... to adequately address the needs of patients and their families, shaped my desire to ...
(Date:11/24/2015)... ... ... All her life, Don Peck’s mother wondered if she was a descendant of Samuel Fuller, ... After a 25-year search for information, Don and his aunt discovered that she was not, ... Turns out, it was Don’s father who was descended from not one, but four passengers ...
Breaking Medicine News(10 mins):
(Date:11/24/2015)... PUNE, India , November 24, 2015 ... new market research report "Spine Biologics Market by Product Type ... Type (Anterior Cervical Discectomy and Fusion, Posterior Lumbar Interbody Fusion), ... by MarketsandMarkets, the global market was valued at $1.90 Billion ... 2020, at a CAGR of 4.4% during the forecast period ...
(Date:11/24/2015)... , Nov. 24, 2015 Avery Biomedical Devices ... pleased to announce the appointment of Anders Jonzon ... Dr. Jonzon is ... at Children,s Hospital, Uppsala University, Uppsala and Children,s Hospital, ... he was a fellow at the Cardiovascular Institute (UCSF). ...
(Date:11/24/2015)... 24, 2015 . ... adds Latest Guidebook for Chinese Medical Device ... published in November 2015 to the medical devices ... library at . ... growing global economies with a fifth population in ...
Breaking Medicine Technology: