Navigation Links
Researchers use immunocytochemistry to determine ALK status
Date:7/1/2013

DENVER Personalized medicine in lung cancer relies on the identification and characterization of cancer biomarkers and the availability of accurate detection systems and therapies for those biomarkers. The standard procedure for detection of predictive anaplastic lymphoma kinase (ALK)-rearrangements is fluorescence in situ hybridization (FISH), but FISH is both expensive and often challenging to interpret. Lung cancer is often diagnosed by cytology necessitating predictive molecular marker analyses on cytological specimens.

Now research published in the August issue of the Journal of Thoracic Oncology (JTO), says ALK immunocytochemistry is highly accurate for detecting ALK-rearranged non-small cell lung cancer (NSCLC) on cytological specimens.

Researchers retrospectively analyzed 41 specimens with available ALK FISH results with the 5A4 monoclonal antibody on a fully automated slide stained. The study population was enriched for consecutive ALK FISH-positive NSCLCs. Eighteen consecutive ALK FISH-negative NSCLCs with EGFR and KRAS wild-type were included as negative controls. Because ALK rearrangements are practically mutually exclusive to other known driver mutations, nine consecutive NSCLCs with EGFR mutations were also analyzed by ALK FISH and included as negative controls. The sensitivity, specificity and positive and negative predictive values for ALK immunocytochemistry compared to ALK FISH were 93.3 percent, 96 percent, 93.3 percent and 96 percent, respectively.

The researchers conclude that, cytological NSCLC specimens are well suited for ALK rearrangement testing. ALK ICC with the 5A4 mAb is feasible and highly accurate for the detection of ALK-rearranged NSCLC on conventional cytological specimens and cellblock preparations and can be used for prescreening NSCLCs.


'/>"/>

Contact: Kristal Griffith
Kristal.Griffith@iaslc.org
720-325-2952
International Association for the Study of Lung Cancer
Source:Eurekalert

Page: 1

Related medicine news :

1. Researchers find 2 new methods to determine ALK status
2. CWRU researchers trace inner-city womens health issues to childhood traumas
3. Penn Medicine researchers discover link between fear and sound perception
4. UCI researchers awarded $2.27 million to create novel diabetes treatments
5. MS researchers determine that brain reserve independently protects against cognitive decline in MS
6. Notre Dame and Harper researchers developing novel method to test for HPV and oral cancers
7. PhD Thesis Writing Expands the Scope of its Services for Doctoral Researchers
8. Researchers Identify New Genes Linked to the Origins of Alzheimer’s, Says Cure Alzheimers Fund
9. Researchers identify master coordinator for aortic rupture
10. Fate of the heart: Researchers track cellular events leading to cardiac regeneration
11. Moffitt Cancer Center researchers identify genetic variants predicting aggressive prostate cancers
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... ... ... PawPaws brand pet supplements owned by Whole Health Supply is ... of felines. The formula is all-natural and is made from Chinese herbs that have ... Kidney Support Supplement Soft Chews are Astragalus Root Extract and Rehmannia Root Extract ...
(Date:6/25/2016)... ... 2016 , ... The temporary closing of Bruton Memorial Library on June 21 due to a ... new, often overlooked aspect of head lice: the parasite’s ability to live away from a ... occurrence, but a necessary one in the event that lice have simply gotten out of ...
(Date:6/25/2016)... ... June 25, 2016 , ... On Friday, June 10, Van ... Wellness at Work award to iHire in recognition of their exemplary accomplishments in worksite ... 7th annual Maryland Workplace Health & Wellness Symposium at the BWI Marriott in Linthicum ...
(Date:6/24/2016)... ... , ... Marcy was in a crisis. Her son James, eight, was out of control. Prone ... physically. , “When something upset him, he couldn’t control his emotions,” remembers Marcy. “If ... at my other children and say he was going to kill them. If we ...
(Date:6/24/2016)... ... ... Topical BioMedics, Inc, makers of Topricin and MyPainAway Pain Relief Products, join The ‘Business for ... $12 an hour by 2020 and then adjusting it yearly to increase at the same ... wage, assure the wage floor does not erode again, and make future increases more predictable. ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... 24, 2016  Collagen Matrix, Inc., ("Collagen Matrix") ... manufacturing of collagen and mineral based medical devices ... Bill Messer has joined the company ... leverage the growing portfolio of oral surgery, neurosurgery, ... Bill joins the Collagen Matrix executive team as ...
(Date:6/24/2016)... 2016 Research and Markets ... Electronics 2015-2025: Applications, Technologies, Forecasts" report to ... In-Mold Electronics, Smart Skin, Structural Health Monitoring, Composite ... Structural electronics involves electronic and/or electrical components and ... dumb structures such as vehicle bodies or conformally ...
(Date:6/24/2016)... Research and Markets has announced the addition of the ... to their offering. ... World Market for Companion Diagnostics covers the world market for ... report includes the following: , World IVD ... (N. America, EU, ROW), 2015-2020 , World IVD Companion ...
Breaking Medicine Technology: