Navigation Links
Researchers use immunocytochemistry to determine ALK status
Date:7/1/2013

DENVER Personalized medicine in lung cancer relies on the identification and characterization of cancer biomarkers and the availability of accurate detection systems and therapies for those biomarkers. The standard procedure for detection of predictive anaplastic lymphoma kinase (ALK)-rearrangements is fluorescence in situ hybridization (FISH), but FISH is both expensive and often challenging to interpret. Lung cancer is often diagnosed by cytology necessitating predictive molecular marker analyses on cytological specimens.

Now research published in the August issue of the Journal of Thoracic Oncology (JTO), says ALK immunocytochemistry is highly accurate for detecting ALK-rearranged non-small cell lung cancer (NSCLC) on cytological specimens.

Researchers retrospectively analyzed 41 specimens with available ALK FISH results with the 5A4 monoclonal antibody on a fully automated slide stained. The study population was enriched for consecutive ALK FISH-positive NSCLCs. Eighteen consecutive ALK FISH-negative NSCLCs with EGFR and KRAS wild-type were included as negative controls. Because ALK rearrangements are practically mutually exclusive to other known driver mutations, nine consecutive NSCLCs with EGFR mutations were also analyzed by ALK FISH and included as negative controls. The sensitivity, specificity and positive and negative predictive values for ALK immunocytochemistry compared to ALK FISH were 93.3 percent, 96 percent, 93.3 percent and 96 percent, respectively.

The researchers conclude that, cytological NSCLC specimens are well suited for ALK rearrangement testing. ALK ICC with the 5A4 mAb is feasible and highly accurate for the detection of ALK-rearranged NSCLC on conventional cytological specimens and cellblock preparations and can be used for prescreening NSCLCs.


'/>"/>

Contact: Kristal Griffith
Kristal.Griffith@iaslc.org
720-325-2952
International Association for the Study of Lung Cancer
Source:Eurekalert

Page: 1

Related medicine news :

1. Researchers find 2 new methods to determine ALK status
2. CWRU researchers trace inner-city womens health issues to childhood traumas
3. Penn Medicine researchers discover link between fear and sound perception
4. UCI researchers awarded $2.27 million to create novel diabetes treatments
5. MS researchers determine that brain reserve independently protects against cognitive decline in MS
6. Notre Dame and Harper researchers developing novel method to test for HPV and oral cancers
7. PhD Thesis Writing Expands the Scope of its Services for Doctoral Researchers
8. Researchers Identify New Genes Linked to the Origins of Alzheimer’s, Says Cure Alzheimers Fund
9. Researchers identify master coordinator for aortic rupture
10. Fate of the heart: Researchers track cellular events leading to cardiac regeneration
11. Moffitt Cancer Center researchers identify genetic variants predicting aggressive prostate cancers
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... ... June 27, 2016 , ... ... announced its strategic partnership with Connance, a healthcare industry leader providing predictive ... proven, proprietary technology combine to provide health systems, hospitals and ambulatory surgical ...
(Date:6/26/2016)... (PRWEB) , ... June 26, 2016 , ... Pixel Film ... Pro X. , "Film editors can give their videos a whole new perspective by ... Austin - CEO of Pixel Film Studios. , ProSlice Levels contains over 30 ...
(Date:6/25/2016)... ... 25, 2016 , ... Austin residents seeking Mohs surgery services, can now turn ... to Dr. Russell Peckham for medical and surgical dermatology. , Dr. Dorsey brings specialization ... selective fellowship in Mohs Micrographic Surgery completed by Dr. Dorsey was under the direction ...
(Date:6/25/2016)... ... June 25, 2016 , ... ... non-athletes recover from injury. Recently, he has implemented orthobiologic procedures as a method ... —Johnson is one of the first doctors to perform the treatment. Orthobiologics are ...
(Date:6/24/2016)... ... ... June 19, 2016 is World Sickle Cell Observance Day. In an effort to ... treatments, Serenity Recovery Center of Marne, Michigan, has issued a pain management ... (SCD) is a disorder of the red blood cells, which can cause episodes of ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... , June 23, 2016 , ... Thursday, July 7, 2016 , , , , LOCATION: , ... , , , , EXPERT PANELISTS:  , , , Frost ... Industry Analyst, Christi Bird; Senior Industry Analyst, Divyaa Ravishankar and Unmesh ... The global pharmaceutical industry is witnessing an exceptional era. Several ...
(Date:6/23/2016)... N.J. , June 23, 2016  Guerbet announced ... Premier Inc.,s Supplier Horizon Award . ... year, Guerbet was recognized for its support of Premier ... creation through clinical excellence, and commitment to lower costs. ... to receive this recognition of our outstanding customer service ...
(Date:6/23/2016)... , June 23, 2016 ... but it continues to present great opportunities to investors. ... for today: Intrexon Corp. (NYSE: XON ), ... Pharmaceuticals Inc. (NASDAQ: ARNA ), and Regeneron ... about these stocks and receive your complimentary trade alerts ...
Breaking Medicine Technology: