Navigation Links
Researchers unlock the protein puzzle
Date:7/23/2014

By using brightly hued dyes, George Mason University researchers discovered an innovative way to reveal where proteins touch each other, possibly leading to new treatments for cancer, arthritis, heart disease and even lung disease.

George Mason researchers unraveled the mystery of deciphering the contact points where proteins touch each other. "One protein interlocks with another protein like adjacent pieces in a jigsaw puzzle, and this sends a signal down the line to the next protein," says Lance Liotta, co-director of the Mason-based Center for Applied Proteomics and Molecular Medicine.

The mystery is in the "hot spots" where proteins interlock. Researchers know which proteins connect but couldn't pinpoint where it happens. Until now, thanks to Mason's newly published approach.

Dyesthe type used in common copying machines and textilesare mixed with proteins. The dye paints the proteins everywhere except where the proteins are connected to one another. Then the proteins are disconnected but the dye remains, excluding the blank spot where the proteins were "kissing."

Finding ways to break up interlocking proteins could be used to locate new drug targets, says Virginia "Ginny" Espina, a professor with the center. Pharmaceutical companies could use the Mason-developed process to create drugs that break up the protein-to-protein connection or stop it from happening altogether, she says.

The team tackled a complex interaction of three proteins, called interleukin signaling, that leads to painful inflammatory arthritis and other diseases including inflammatory bowel disease. They created two inhibitorsa peptide and an antibodythat broke up the protein connection in a test tube. "Both inhibitors made these proteins fall apart and they couldn't send out a signal for inflammation," Liotta says.

Until the Mason-led advancement, researchers have struggled to figure out where proteins make contact. "It seems very easy but, in reality, it's not," says Alessandra Luchini, a professor with the proteomics center who created the experimental method.

Researchers have used computer modeling and crystalized proteins but couldn't show proteins making contact in real time, she says. "Using this tool, we now can study the protein exactly as it's found in nature," Luchini says.

And as it turns out, the printer dyes not only paint a pretty picture, but they are the perfect size to color the proteinsand they stick. The Mason team is using blue, red, purple and orange dyes.


'/>"/>
Contact: Michele McDonald
mmcdon15@gmu.edu
703-993-8781
George Mason University
Source:Eurekalert

Related medicine news :

1. Communication about female condom vital to young adults, UT Arlington researchers say
2. Feinstein Institute researchers identify brain network
3. CNIO researchers discover a gene that links stem cells, ageing and cancer
4. University of Houston researchers create new method to draw molecules from live cells
5. Researchers Spot Potential New Culprit Behind Alzheimers
6. Researchers demonstrate health risks posed by third hand tobacco smoke
7. Chinese researchers describe impaired self-face recognition in those with major depressive disorder
8. UC Davis Researchers' Discovery Has Implications for Developing Treatments for Deafness
9. Georgia State Researchers Discover Hidden Variations in Neuronal Networks, May Explain Differences In Traumatic Brain Injury Outcomes
10. RSNA: Researchers Assess Emergency Radiology Response After Boston Marathon Bombings
11. Researchers assess emergency radiology response after Boston Marathon bombings
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... (PRWEB) , ... October 13, 2017 , ... While it’s ... poses a problem. Fortunately, an inventor from Austin, Texas, has identified a solution. , ... medication in darkness or restricted lighting. As such, it eliminates the need to turn ...
(Date:10/13/2017)... ... October 13, 2017 , ... Lori R. Somekh, founder ... of ElderCounsel, a national organization of elder law and special needs planning attorneys. “Membership ... rules. It also provides a forum to network with elder law attorneys nationwide,” said ...
(Date:10/13/2017)... ... ... Global Healthcare Management’s 4th Annual Kids Fun Run brought out many kids this ... by Global Healthcare Management’s CEO, Jon Letko, is aimed at getting kids excited about ... ages; it is a non-competitive, non-timed event, which is all about having fun and ...
(Date:10/13/2017)... ... October 13, 2017 , ... “America On The Brink”: the Christian history of ... Brink” is the creation of published author, William Nowers. Captain Nowers and his ... veteran, he spent thirty years in the Navy. Following his career as a ...
(Date:10/12/2017)... ... , ... IsoComforter, Inc. ( https://isocomforter.com ), one of the Nation’s ... design of the shoulder pad. The shoulder pad provides optimal support and full ... while using cold therapy. By utilizing ice and water that is circulated from an ...
Breaking Medicine News(10 mins):
(Date:10/12/2017)... , Oct. 12, 2017 West ... in innovative solutions for injectable drug administration, today announced ... the market opens on Thursday, October 26, 2017, and ... results and business expectations at 9:00 a.m. Eastern Time. ... or 253-336-8738 (International). The conference ID is 94093362. ...
(Date:10/11/2017)...  True Health, a leader in integrated diagnostics ... National Breast Cancer Awareness month to educate doctors ... Research recently published in ... than 10 million American women are at significant ... BRCA2 and have not had testing. These mutations can ...
(Date:10/10/2017)... Oct. 10, 2017   West Pharmaceutical Services, Inc. ... for injectable drug administration, today shared the results of ... for improving the intradermal administration of polio vaccines. The ... Summit in May 2017 by Dr. Ondrej Mach ... World Health Organization (WHO), and recently published in the ...
Breaking Medicine Technology: