Navigation Links
Researchers find novel approach for controlling deadly C. difficile infections
Date:1/30/2014

Researchers from the Alberta Glycomics Centre at the University of Calgary and the University of Alberta, in collaboration with researchers at the National Research Council of Canada in Ottawa, have revealed the first molecular views showing how highly specific antibodies derived from llamas may provide a new method for controlling deadly infections from the opportunistic bacterial pathogen Clostridium difficile (C. difficile).

Published in the Journal of Biological Chemistry, the research reveals for the first time how antibodies recognize the disease's two central toxin proteins toxin A (TcdA) and toxin B (TcdB).

This new information provides exciting opportunities for creating a new generation of engineered antibodies that will be more effective at preventing the toxins from damaging the intestine during the normal course of the disease.

"Our research is an important step towards developing highly specific ways to treat this very common and serious disease," says Kenneth Ng, associate professor in the Department of Biological Sciences at the University of Calgary and the study's senior author.

C. difficile causes one of the most common and problematic hospital-acquired infections worldwide. The infection is primarily transmitted within healthcare facilities, and causes extreme diarrhea and potentially fatal colon inflammation.

A Canadian hospital study found that of 136,877 hospital admissions, 1 in 100 patients will contract C. difficile infection, and of those, 1 in 10 will die regardless of the initial reasons for admission. The disease is most frequently seen in older adults who take antibiotics and get medical care. Annual healthcare costs are estimated to be several billion dollars worldwide.

The key findings in the paper derive from the three-dimensional structures of antibody-toxin complexes that were determined using X-ray crystallography by Tomohiko Murase, Luiz Eugenio and Melissa Schorr in Dr. Ng's laboratory.

The antibody-toxin complexes were developed using single-domain antibodies derived from llamas.

"The smaller size of the llama antibodies compared to the monoclonal antibodies currently used for diagnostics or in development for therapeutics greatly assists with structure determination and protein engineering," explains Ng. "Starting from these structures, we are now creating modified antibodies for improving treatments in the future."

"Basic biological research on llamas, camels and sharks led to the discovery of a smaller type of antibody with a simpler structure," adds Ng. "It is this simpler structure that allows us to make modifications and perform many detailed studies that are not easily done with other types of antibodies.

The unique characteristics of these single-domain antibodies provide an attractive approach for developing new treatments for C. difficile."

According to Ng, although the research is at the fundamental science level, the new structures provide a blueprint for designing new molecules that could neutralize the bacterial toxins more effectively than anything currently available.

This project relied on important contributions from Elena Kitova in John Klassen's mass spectrometry group at the Alberta Glycomics Centre, at the University of Alberta, as well as from Greg Hussack in Jamshid Tanha's antibody therapeutics group at the National Research Council in Ottawa.


'/>"/>

Contact: Marie-Helene Thibeault
m.thibeault@ucalgary.ca
403-679-8447
University of Calgary
Source:Eurekalert  

Related medicine news :

1. NIH awards $20 million over 5 years to train next generation of global health researchers
2. Researchers develop a new cell and animal model of inflammatory breast cancer
3. Researchers uncover a viable way for colorectal cancer patients to overcome drug resistance
4. Researchers Find Gene Mutations That May Be a Key to Autism
5. Researchers find evidence of banned antibiotics in poultry products
6. NJ stroke researchers report advances in spatial neglect research at AAN Conference
7. Autism by the numbers: Yale researchers examine impact of new diagnostic criteria
8. Researchers Map Brain Regions Linked to Intelligence
9. Researchers ID Genes That May Determine Mental Illness
10. Researchers Develop Blood Test for Depression
11. University of Cincinnati researchers win $3.7M grant from US Department of Defense
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers find novel approach for controlling deadly C. difficile infections
(Date:12/4/2016)... (PRWEB) , ... December 02, 2016 , ... ... will give patients better access to nutraceuticals and help doctors more efficiently manage ... patient portal and practice management software platform for Integrative and Functional Medicine. ...
(Date:12/4/2016)... ... December 03, 2016 , ... ... at the 2016 Anti-Aging & Beauty Awards at The Aesthetic & ... & Anti-Aging Medicine European Congress (AMEC) brings together the industry’s leading scientific ...
(Date:12/4/2016)... ... December 04, 2016 , ... Are You Concerned About Mold ... certified and experienced Indoor Air Quality Companies in VA, MD and DC, recently completed ... , The new mold law in the district of Columbia is a good ...
(Date:12/2/2016)... (PRWEB) , ... December 02, 2016 , ... ... documentary on the perils of heroin that was watched live by 1 million ... Alfred I. duPont-Columbia University Award. , ASU students at the Walter Cronkite School ...
(Date:12/2/2016)... ... , ... Advanced Inc., a leading provider of travel therapy and travel nursing ... as Advanced Inc.’s Chief Financial Officer, effective December 1, 2016. Jason previously served as ... operational leadership experience to Advanced Inc. He began his career in finance at Ernst ...
Breaking Medicine News(10 mins):
(Date:12/5/2016)... Israel , December 5, 2016 ... identifying, in-licensing and developing promising therapeutic candidates, presents positive Phase ... BL-8040, the Company,s leading oncology platform at the ongoing 58th ... San Diego, California . ... , , ...
(Date:12/5/2016)... Taiwan , Dec. 5, 2016 ... non-inferiority to hydroxyurea (HU) in Complete Hematologic Response (CHR) ... profile of ropeginterferon alfa-2b versus HU AOP ... follow-up trial CONTINUATION-PV to obtain European marketing authorization in the ... data to the FDA as it seeks approval for commercialization ...
(Date:12/4/2016)... --  Pairnomix, LLC, a genetic research company and member ... White House, today announced that findings from its first case ... therapies for a patient with epileptic encephalopathy caused by a ... the 70 th Annual Meeting of the American ... December 2-6, 2016.  Pairnomix, unique process involves creating ...
Breaking Medicine Technology: