Navigation Links
Researchers find novel approach for controlling deadly C. difficile infections
Date:1/30/2014

Researchers from the Alberta Glycomics Centre at the University of Calgary and the University of Alberta, in collaboration with researchers at the National Research Council of Canada in Ottawa, have revealed the first molecular views showing how highly specific antibodies derived from llamas may provide a new method for controlling deadly infections from the opportunistic bacterial pathogen Clostridium difficile (C. difficile).

Published in the Journal of Biological Chemistry, the research reveals for the first time how antibodies recognize the disease's two central toxin proteins toxin A (TcdA) and toxin B (TcdB).

This new information provides exciting opportunities for creating a new generation of engineered antibodies that will be more effective at preventing the toxins from damaging the intestine during the normal course of the disease.

"Our research is an important step towards developing highly specific ways to treat this very common and serious disease," says Kenneth Ng, associate professor in the Department of Biological Sciences at the University of Calgary and the study's senior author.

C. difficile causes one of the most common and problematic hospital-acquired infections worldwide. The infection is primarily transmitted within healthcare facilities, and causes extreme diarrhea and potentially fatal colon inflammation.

A Canadian hospital study found that of 136,877 hospital admissions, 1 in 100 patients will contract C. difficile infection, and of those, 1 in 10 will die regardless of the initial reasons for admission. The disease is most frequently seen in older adults who take antibiotics and get medical care. Annual healthcare costs are estimated to be several billion dollars worldwide.

The key findings in the paper derive from the three-dimensional structures of antibody-toxin complexes that were determined using X-ray crystallography by Tomohiko Murase, Luiz Eugenio and Melissa Schorr in Dr. Ng's laboratory.

The antibody-toxin complexes were developed using single-domain antibodies derived from llamas.

"The smaller size of the llama antibodies compared to the monoclonal antibodies currently used for diagnostics or in development for therapeutics greatly assists with structure determination and protein engineering," explains Ng. "Starting from these structures, we are now creating modified antibodies for improving treatments in the future."

"Basic biological research on llamas, camels and sharks led to the discovery of a smaller type of antibody with a simpler structure," adds Ng. "It is this simpler structure that allows us to make modifications and perform many detailed studies that are not easily done with other types of antibodies.

The unique characteristics of these single-domain antibodies provide an attractive approach for developing new treatments for C. difficile."

According to Ng, although the research is at the fundamental science level, the new structures provide a blueprint for designing new molecules that could neutralize the bacterial toxins more effectively than anything currently available.

This project relied on important contributions from Elena Kitova in John Klassen's mass spectrometry group at the Alberta Glycomics Centre, at the University of Alberta, as well as from Greg Hussack in Jamshid Tanha's antibody therapeutics group at the National Research Council in Ottawa.


'/>"/>

Contact: Marie-Helene Thibeault
m.thibeault@ucalgary.ca
403-679-8447
University of Calgary
Source:Eurekalert  

Related medicine news :

1. NIH awards $20 million over 5 years to train next generation of global health researchers
2. Researchers develop a new cell and animal model of inflammatory breast cancer
3. Researchers uncover a viable way for colorectal cancer patients to overcome drug resistance
4. Researchers Find Gene Mutations That May Be a Key to Autism
5. Researchers find evidence of banned antibiotics in poultry products
6. NJ stroke researchers report advances in spatial neglect research at AAN Conference
7. Autism by the numbers: Yale researchers examine impact of new diagnostic criteria
8. Researchers Map Brain Regions Linked to Intelligence
9. Researchers ID Genes That May Determine Mental Illness
10. Researchers Develop Blood Test for Depression
11. University of Cincinnati researchers win $3.7M grant from US Department of Defense
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers find novel approach for controlling deadly C. difficile infections
(Date:2/24/2017)... ... ... The International Association of Eating Disorders Professionals (iaedp) announces the 2017 top five ... art competition. Selected from 15 submissions from around the nation, the top five finalists ... iaedp Symposium, March 22 – 26 in Las Vegas. , This year, the competition ...
(Date:2/24/2017)... ... February 24, 2017 , ... Castle ... hosted their Military Wedding Giveaway, with the winning couple announced on Feb. 14, ... services generously donated from local vendors: A Matter of Taste, Ryan Rousseau Enterprises, ...
(Date:2/23/2017)... ... February 23, 2017 , ... ... a clinician-based audience, will be participating in Rare Disease Day events, hosted by ... In addition, Rare Disease Report, a website, weekly e-newsletter and quarterly publication, will ...
(Date:2/23/2017)... Mass. (PRWEB) , ... February 23, 2017 , ... ... as an approved content provider for the National Institute for Health and ... and social care-related organizations in the National Health Service (NHS) to search, order ...
(Date:2/23/2017)... , ... February 23, 2017 , ... ... announced today that it will soon begin franchising throughout the U.S. starting this ... to help bring the practice of meditation mainstream. Current Meditation will be the ...
Breaking Medicine News(10 mins):
(Date:2/24/2017)... BRISBANE, Australia , Feb. 23, 2017  Directors from Pharma ... the two companies have joined forces, resulting in the founding of ... . Pharma To Market are pleased to announce their ... an office in Singapore . The company are ... Consulting as Co-Director of the Singapore based ...
(Date:2/24/2017)... Feb 24, 2017 Medivir AB ... for a new Board of Directors that will be ... Nomination Committee comprises representatives of the company,s three largest ... 2016 who have accepted a seat on the Nomination ... of the 2016-2017 Nomination Committee was as follows:  ...
(Date:2/23/2017)... Research and Markets has announced the addition of the "Autism ... to their offering. ... The latest research Autism Spectrum Disorder Drugs Price Analysis and ... global Autism Spectrum Disorder market. The research answers the following ... marketed for Autism Spectrum Disorder and their clinical attributes? How are ...
Breaking Medicine Technology: