Navigation Links
Researchers find 2 new methods to determine ALK status
Date:7/1/2013

DENVER The implementation of personalized health care in cancer relies on the identification and characterization of cancer biomarkers and the availability of accurate detection systems and therapies for those biomarkers. Anaplastic lymphoma kinase (ALK), a tyrosine kinase, is a more recently characterized cancer biomarker in nonsmall-cell lung cancer (NSCLC). To identify NSCLC patients with ALK gene rearrangement in clinical trials, researchers have used the methods known as fluorescence in situ hybridization (FISH) or immunohistochemistry (IHC). While IHC is a less complex and less costly technology than FISH, both methods present challenges.

Now research published in the August issue of the Journal of Thoracic Oncology (JTO), describes the development and evaluation of two new methodologies.

To improve IHC assay sensitivity, the researchers incorporated the novel, nonendogenous hapten 3-hydroxy-2-quinoxaline and tyramide amplification into a diaminobenzidine and horseradish peroxidasebased assay. The new detection system proved to be very useful for detecting low levels of ALK protein expression in NSCLC.

They also developed a brightfield IHCin situ hybridization combination assay (geneprotein assay) for the concurrent visualization of ALK protein and ALK gene arrangement. This allows the concurrent visualization of ALK gene and ALK protein status in single cells, allowing more accurate ALK status determination even in heterogeneous specimens.

The authors say, "this tool for simultaneously assessing both ALK protein expression (IHC) and ALK gene rearrangement (ISH) in NSCLC will be valuable for research on the mechanisms driving ALK-dependent malignancies and as a model of new diagnostic approach for identifying patients who might benefit from ALK-targeted therapies. More generally, it also provides proof of concept for the development of new methodologies for the simultaneous assessment of gene structure and protein-expression status in a single cell.


'/>"/>

Contact: Kristal Griffith
Kristal.Griffith@iaslc.org
720-325-2952
International Association for the Study of Lung Cancer
Source:Eurekalert

Related medicine news :

1. CWRU researchers trace inner-city womens health issues to childhood traumas
2. Penn Medicine researchers discover link between fear and sound perception
3. UCI researchers awarded $2.27 million to create novel diabetes treatments
4. MS researchers determine that brain reserve independently protects against cognitive decline in MS
5. Notre Dame and Harper researchers developing novel method to test for HPV and oral cancers
6. PhD Thesis Writing Expands the Scope of its Services for Doctoral Researchers
7. Researchers Identify New Genes Linked to the Origins of Alzheimer’s, Says Cure Alzheimers Fund
8. Researchers identify master coordinator for aortic rupture
9. Fate of the heart: Researchers track cellular events leading to cardiac regeneration
10. Moffitt Cancer Center researchers identify genetic variants predicting aggressive prostate cancers
11. Timely treatment after stroke is crucial, UCLA researchers report
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/12/2016)... , ... February 12, 2016 , ... ... devoted exclusively to funding innovative lymphoma research and serving the lymphoma community through ... over 250 members of South Florida’s philanthropic community at its 10th anniversary Fashion ...
(Date:2/12/2016)... ... , ... As a former television executive, owner Tal Rabinowitz knows how stressful ... decompress, Rabinowitz found herself drawn to a casual meditation class while working at NBC ... implementing a 20-minute-per-day meditation practice with her team. After her tenure at NBC, she ...
(Date:2/12/2016)... Miami, FL (PRWEB) , ... February 12, 2016 ... ... Workspace as a Service (WaaS), today announced the integration of Clarity Intelligence Platform ... itopia’s channel partners to offer real-time business intelligence (BI) to their small and ...
(Date:2/12/2016)... ... ... The Jones Agency, a family owned insurance company with offices serving Arlington, Dallas, ... with the Tarrant Area Food Bank in the hopes of alleviating hunger for nearby ... Tarrant County Food Bank offers hope and security to the one in six Texas ...
(Date:2/12/2016)... , ... February 12, 2016 , ... Donor Network West, ... California and Nevada, announced a partnership with San Ramon Regional Medical Center. Under the ... hospital’s facilities as a way to accommodate a more certain time frame for donor ...
Breaking Medicine News(10 mins):
(Date:2/11/2016)... -- The primary goal of this research is to ... usage of liquid biopsy. Key information the survey seeks ... of liquid biopsy adoption amidst future users - Predominantly ... - Sample inflow to conduct liquid biopsy tests - ... so on. - Correlation analysis of sample type and ...
(Date:2/11/2016)... SAN FRANCISCO , Feb. 11, 2016  Kindred ... focused on saving and improving the lives of pets, ... Technical Section of the New Animal Drug Application (NADA) ... the pivotal field study (KB0120) of Zimeta for the ... by the Company. --> ...
(Date:2/11/2016)... Feb. 11, 2016 Potrero Medical, Inc., the developer ... announce the appointment of George M. Rapier, III , ... Antonio, TX , WellMed is one of the nation,s ... and HMO members in Texas and ... out of his own internal medicine practice, he has been ...
Breaking Medicine Technology: