Navigation Links
Researchers develop a new cell and animal model of inflammatory breast cancer
Date:4/4/2012

CHICAGO, IL (April 4, 2012)Inflammatory breast cancer (IBC) is a very aggressive, often misunderstood type of cancer that is diagnosed more frequently in younger women compared with other types of breast cancer. The five-year survival rate is between 25 and 50 percentsignificantly lower than the survival rate for other types of breast cancer. The reason for the poor prognosis is that IBC usually grows rapidly and often spreads quickly to other parts of the body, including the brain, bone and lymph nodes. In an effort to better understand the biology of IBC, researchers at Fox Chase Cancer Center have developed a new cell and animal model that holds promise for providing a detailed understanding of the molecular mechanisms underlying the disease and for developing effective interventions.

"In order for us to improve the treatment of these patients, we need to understand the biology of the diseasewhy these cells are so aggressive, invade very early on, and are resistant to standard treatmentsand this starts with having good laboratory and preclinical models," says Massimo Cristofanilli, M.D., F.A.C.P., chairman of Fox Chase's department of medical oncology and senior investigator for the research, which will be presented at the AACR Annual Meeting 2012 on Wednesday, April 4.

The researchers developed a unique model that recapitulates the aggressive metastasis and cancer stem cell activity associated with poor outcomes in patients with IBC. Understanding of the molecular basis of IBC may help increase the research community's knowledge of the metastatic process of other types of breast cancers.

"Because there are only a few models of inflammatory breast cancer, it's important to develop more models of this disease, and ours represents an ideal model to evaluate stem cell-targeting therapies," says Sandra Fernandez, Ph.D., assistant research professor at Fox Chase and lead author on the study.

To develop the new disease model, Fernandez, Cristofanilli and their colleagues developed an IRB-approved prospective protocol allowing for the collection of tissue and pleural fluid from patients with advanced IBC. The new cell line, known as FC-IBC02, was established from the pleural fluid collected from a 49-year-old patient whose cells lacked the protein HER2/neu, as well as receptors for the female hormones estrogen and progesterone. About 15 percent of breast cancer patients share these features and, as a result, they do not respond to hormonal therapies and certain medications that target these proteins. "Currently, the only option to treat these patients is chemotherapy," Fernandez says. "So it's important to have a specific model that we can use to test different drugs and see which ones work for this kind of disease."

Moreover, the researchers grew culture tumor cells derived from the patient's fluid and found that they contained a large amount of the protein tetraspanin CD151, which controls tumor cell migration and invasion. In addition, these cells formed multicellular spheroids that displayed markers of cancer stem cells, including the marker CD44. When injected into the mammary fat pad of mice, the tumor spheroids rapidly developed into tumors and spread to the lungs.

Furthermore, using the latest CytoScan HD arrays, the FCCC researchers found that these cells have multiple losses and gains across almost the whole genome, a phenomenon known as chromothripsis. In particular, FC-IBC02 cells have an amplification on chromosome 8q where the oncogene MYC is located and a deletion on chromosome 7p where tumor suppressor gene p53 is embedded. These analysis will identify novel molecular targets to fight the disease. By culturing cells from a large pool of patients, they will look for promising targets that are commonly associated with IBC as well as test new stem cell-targeting drugs that could reduce metastasis.

"I think it's a major step forward for us as clinicians and scientists to develop better therapies and new diagnostic tools for patients with inflammatory breast cancer," Cristofanilli says. "We would like to translate our discoveries from bench to bedside very quickly, as these patients really need new treatments."


'/>"/>

Contact: Diana Quattrone
Diana.Quattrone@fccc.edu
215-815-7828
Fox Chase Cancer Center
Source:Eurekalert

Related medicine news :

1. NIH awards $20 million over 5 years to train next generation of global health researchers
2. Researchers identify a gene that predicts recurrence in squamous cell carcinoma of the head and neck
3. Researchers uncover new clues to the development of blood and other cancers
4. Researchers work to help soldiers break camouflage
5. Researchers identify mechanism that makes breast cancer invasive
6. Penn researchers call for a re-examination of transplant waitlist prioritization
7. Nemours researchers uncover new evidence of cancer-causing agent present in gaseous phase of cigarette smoke
8. NIH researchers highlight progress, path forward for developing TB vaccines
9. Penn researchers find mentoring provides health benefits for African American veterans with diabetes
10. Mayo Clinic researchers building melanoma vaccine to combat skin cancer
11. Evidence builds that meditation strengthens the brain, UCLA researchers say
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/17/2019)... ... July 17, 2019 , ... Women’s Excellence is pleased to announce a new office opening ... The new offices resides on the corner of Turrill Road and South Lapeer Roads, ... thrilled about the opening of our new office in Lapeer and the ability to service ...
(Date:7/13/2019)... ... July 12, 2019 , ... The Oncology Institute ... office is located at 5380 S. Rainbow Blvd., Suite 210, Las Vegas, NV ... cancer center is equipped with state-of-the-art medical equipment plus all of the top-notch ...
(Date:7/13/2019)... ... July 12, 2019 , ... The American Med Spa Association (AmSpa) has released ... help medical aesthetic and medical spa professionals understand the realities of the industry and ... “It’s been two years since AmSpa released the 2017 Medical Spa State of the ...
(Date:7/11/2019)... ... July 11, 2019 , ... The ... educational resources with a range of features to further advance their mission of ... industry in manufacturing quality medicines for patients worldwide. The latest additions include a ...
(Date:7/10/2019)... ... July 10, 2019 , ... HMP, a leader in healthcare events ... the Oncology Clinical Pathways Congress, taking place October 11-13, 2019 in Boston. , Launched ... and focuses on the expansion of clinical pathways as a decision support tool and ...
Breaking Medicine News(10 mins):
(Date:7/17/2019)... ... July 17, 2019 , ... Argos Risk, an ... into the healthcare sector during the second quarter of 2019. Three nationwide companies, ... join a strong client list in managing and mitigating third-party risk. , ...
(Date:7/17/2019)... ... July 17, 2019 , ... For the second year in a row, Astro Pak is ... given by the Orange County Business Journal, which is the second largest business journal in ... County, with the additional honor of ranking third place in the medium size employer category. ...
(Date:7/13/2019)... , ... July 12, 2019 , ... Meet the Pettys, Will and Jamie Petty met ... married in the St. George LDS Temple just six months later. , For the next ... they wanted to start growing their family. In 2015 the decision was made to begin ...
Breaking Medicine Technology: