Navigation Links
Researchers create better methods to detect E. coli
Date:6/16/2014

MANHATTAN, KANSAS Kansas State University diagnosticians are helping the cattle industry save millions of dollars each year by developing earlier and accurate detection of E. coli.

Lance Noll, master's student in veterinary biomedical science, Greensburg; T.G. Nagaraja, university distinguished professor of diagnostic medicine and pathobiology; and Jianfa Bai, assistant professor in the Kansas State Veterinary Diagnostic Laboratory, are leading a project to improve techniques for detecting pathogenic Shiga toxin-producing E. coli O157:H7. A U.S. Department of Agriculture Coordinated Agriculture Project grant is funding the work.

The researchers are part of a College of Veterinary Medicine team studying preharvest food safety in beef cattle. Noll has developed and validated a molecular assay that can detect and quantify major genes specific for E. coli O157.

"Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply," Noll said.

The newly developed test is a molecular assay, or polymerase chain reaction, that detects bacteria based on genetic sequences, which are the bacteria's "fingerprints," Nagaraja said. The test is rapid and less labor-intensive than existing detection methods. The method can be automated and test many samples in a short period of time.

The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities.

"The novelty of this test is that it targets four genes," Nagaraja said. "We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces."

To develop the diagnostic test, Noll and Nagaraja worked with two Kansas State University molecular biologists: Xiaorong Shi, research assistant of diagnostic medicine and pathobiology, and Bai.

"Beef cattle production is a major industry in Kansas and Kansas State University has a rich tradition in the research of beef cattle production and beef safety," Noll said. "As a graduate student in veterinary biomedical sciences, I am proud to be a member of a multidisciplinary team in the College of Veterinary Medicine that aims to make beef a safe product for the consumers."

Noll was a winner at the 11th annual Capitol Graduate Research Summit for his research project and poster, "A four-plex real-time PCR assay for the detection and quantification of Escherichia coli O157 in cattle feces."


'/>"/>

Contact: T.G. Nagaraja
tnagaraj@k-state.edu
785-532-1214
Kansas State University
Source:Eurekalert  

Related medicine news :

1. UGA researchers discover new method to reduce disease-causing inflammation
2. Ottawa researchers key to new neuromuscular disease care and research network
3. Researchers uncover new insights into developing rapid-acting antidepressant for treatment-resistant depression
4. Broad Institute, MGH researchers chart cellular complexity of brain tumors
5. Researchers uncover common heart drugs link to diabetes
6. Mayo Clinic researchers discover new form of cancer
7. Researchers recast addiction as a manageable disease
8. Mount Sinai researchers identify protein that keeps blood stem cells healthy as they age
9. Berkeley Lab researchers create nanoparticle thin films that self-assemble in 1 minute
10. Study shows health policy researchers lack confidence in social media for communication
11. Researchers at the Gladstone Institutes find novel approach to reactivate latent HIV
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers create better methods to detect E. coli
(Date:6/26/2016)... (PRWEB) , ... June 26, 2016 , ... On June ... sponsor of the 2016 Cereal Festival and World’s Longest Breakfast Table in Battle Creek, ... of the city’s history as home to some of the world’s leading providers of ...
(Date:6/25/2016)... ... 25, 2016 , ... Experts from the American Institutes for ... Meeting June 26-28, 2016, at the Hynes Convention Center in Boston. , AIR ... care planning, healthcare costs and patient and family engagement. , AIR researchers will ...
(Date:6/25/2016)... ... June 25, 2016 , ... As a lifelong Southern Californian, Dr. ... his M.D from the David Geffen School of Medicine at UCLA. He trained in ... to complete his fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program where he ...
(Date:6/24/2016)... ... 2016 , ... Those who have experienced traumatic events may suffer from a ... such as drug or alcohol abuse, as a coping mechanism. To avoid this pain ... following a traumatic event. , Trauma sufferers tend to feel a range of emotions, ...
(Date:6/24/2016)... ... June 24, 2016 , ... Dr. Amanda Cheng, an ... Cheng has extensive experience with all areas of orthodontics, including robotic Suresmile technology, ... , Micro-osteoperforation is a revolutionary adjunct to orthodontic treatment. It can be ...
Breaking Medicine News(10 mins):
(Date:6/26/2016)... , June 27, 2016  VMS Rehab Systems, Inc. ... will take whatever measures required to build a strong ... which is currently listed on the OTC Markets-pink current ... Chairman and CEO, "We are seeing an anomaly in ... understand, not only by the Company, but shareholders and ...
(Date:6/24/2016)... 24, 2016   Bay Area Lyme Foundation ... Dean Center for Tick Borne Illness , Harvard ... MIT Hacking Medicine, University of California, Berkeley, and ... the five finalists of Lyme Innovation , ... than 100 scientists, clinicians, researchers, entrepreneurs, and investors ...
(Date:6/24/2016)... and SAN CLEMENTE, Calif. , June 24, 2016 ... mobile pulmonary function testing company, is now able to perform sophisticated ... by ndd Medical Technologies , Inc. Patients ... hospital-based labs.  Thanks to ndd,s EasyOne PRO ® , ARL patients ... get any needed testing done in the comfort of her own ...
Breaking Medicine Technology: