Navigation Links
Researchers create better methods to detect E. coli
Date:6/16/2014

MANHATTAN, KANSAS Kansas State University diagnosticians are helping the cattle industry save millions of dollars each year by developing earlier and accurate detection of E. coli.

Lance Noll, master's student in veterinary biomedical science, Greensburg; T.G. Nagaraja, university distinguished professor of diagnostic medicine and pathobiology; and Jianfa Bai, assistant professor in the Kansas State Veterinary Diagnostic Laboratory, are leading a project to improve techniques for detecting pathogenic Shiga toxin-producing E. coli O157:H7. A U.S. Department of Agriculture Coordinated Agriculture Project grant is funding the work.

The researchers are part of a College of Veterinary Medicine team studying preharvest food safety in beef cattle. Noll has developed and validated a molecular assay that can detect and quantify major genes specific for E. coli O157.

"Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply," Noll said.

The newly developed test is a molecular assay, or polymerase chain reaction, that detects bacteria based on genetic sequences, which are the bacteria's "fingerprints," Nagaraja said. The test is rapid and less labor-intensive than existing detection methods. The method can be automated and test many samples in a short period of time.

The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities.

"The novelty of this test is that it targets four genes," Nagaraja said. "We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces."

To develop the diagnostic test, Noll and Nagaraja worked with two Kansas State University molecular biologists: Xiaorong Shi, research assistant of diagnostic medicine and pathobiology, and Bai.

"Beef cattle production is a major industry in Kansas and Kansas State University has a rich tradition in the research of beef cattle production and beef safety," Noll said. "As a graduate student in veterinary biomedical sciences, I am proud to be a member of a multidisciplinary team in the College of Veterinary Medicine that aims to make beef a safe product for the consumers."

Noll was a winner at the 11th annual Capitol Graduate Research Summit for his research project and poster, "A four-plex real-time PCR assay for the detection and quantification of Escherichia coli O157 in cattle feces."


'/>"/>

Contact: T.G. Nagaraja
tnagaraj@k-state.edu
785-532-1214
Kansas State University
Source:Eurekalert  

Related medicine news :

1. UGA researchers discover new method to reduce disease-causing inflammation
2. Ottawa researchers key to new neuromuscular disease care and research network
3. Researchers uncover new insights into developing rapid-acting antidepressant for treatment-resistant depression
4. Broad Institute, MGH researchers chart cellular complexity of brain tumors
5. Researchers uncover common heart drugs link to diabetes
6. Mayo Clinic researchers discover new form of cancer
7. Researchers recast addiction as a manageable disease
8. Mount Sinai researchers identify protein that keeps blood stem cells healthy as they age
9. Berkeley Lab researchers create nanoparticle thin films that self-assemble in 1 minute
10. Study shows health policy researchers lack confidence in social media for communication
11. Researchers at the Gladstone Institutes find novel approach to reactivate latent HIV
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers create better methods to detect E. coli
(Date:2/5/2016)... ... February 05, 2016 , ... Steven Tonkinson, 36, of Coconut Grove, ... year since it started in 2003. This year, he ran all 26.2 miles with ... and NBA team the Miami Heat. , This Sunday, while many are watching the ...
(Date:2/5/2016)... ... February 05, 2016 , ... The event is being held on April 7, ... Minneapolis, Minn. Triumph Over Parkinson’s will fund nearly $100,000 for research for the care ... with Parkinson’s disease and is the architect of this informative event to raise awareness ...
(Date:2/5/2016)... (PRWEB) , ... February 05, 2016 , ... After years ... General Hospital Burn Unit, plastic and cosmetic surgeon Dr. Wayne Carman transitioned to chief ... Hospital. He successfully completed his first three-year term as chief and began a second ...
(Date:2/5/2016)... York (PRWEB) , ... February 05, 2016 , ... Regular ... and find themselves having to wait longer to access the treadmills. It’s a predictable ... Year’s resolutions to lose weight and get in shape by joining gyms, starting new ...
(Date:2/5/2016)... ... , ... Dr. Justin Scott and Dr. Lydia Muccioli of Pure Dental ... individuals in need. The event is scheduled to take place on February 27, 2016 ... provide dental care to community members in need. Each patient will be given the ...
Breaking Medicine News(10 mins):
(Date:2/5/2016)... 5, 2016 Dehaier Medical Systems Ltd. ... "Company"), which develops, markets and sells medical devices ... China and international markets, today announced ... to concentrate the Company,s resources to develop its ... and to focus more on its major businesses. ...
(Date:2/5/2016)... , Feb. 5, 2016  Henry Schein, Inc. (NASDAQ: ... products and services to office-based dental, animal health and ... an agreement to acquire a majority ownership interest in ... equipment in Brazil . ... Blumenau, Brazil, Dental Cremer is the dental distribution business of ...
(Date:2/5/2016)... 5, 2016 http://www.researchandmarkets.com/research/hcdvg6/global_obstetrics ... "Global Obstetrics Partnering 2010-2016: Deal trends, ... their offering. --> http://www.researchandmarkets.com/research/hcdvg6/global_obstetrics ) ... "Global Obstetrics Partnering 2010-2016: Deal trends, players ... offering. --> Research and Markets ...
Breaking Medicine Technology: