Navigation Links
Reduced Oxygen Levels Could Double Neural Stem Cells’ Chance of Survival
Date:5/15/2013

Durham, NC (PRWEB) May 15, 2013

Decreasing the amount of oxygen traditionally used when culturing stem cells for use in neurological therapies could drastically affect their survival rate. In fact, it could double it, according to a new study released today in STEM CELLS Translational Medicine.

“Cells are usually cultured in the lab in a 20 percent oxygen environment, a level far removed from the in vivo situation. This is particularly true in the central nervous system, where oxygen tensions — that is, the concentration of oxygen at a specific pressure — are normally around 3 percent,” said Sybil Stacpoole, M.D., Ph.D., lead author on the paper by a team of researchers from the Universities of Cambridge and Edinburgh.

“Cell transplantation strategies therefore typically introduce a stress challenge at the time of transplantation as the cells are switched from 20 percent to 3 percent oxygen, which is the average in adult organs,” she added.

A previous study had indicated that cardiac stem cells showed a better survival rate when the oxygen tension during their culturing was reduced. In this study, the Cambridge and Edinburgh teams wanted to learn if the same might prove true for neural stem cells (NSCs). So they modeled the oxygen stress that occurs during transplantation and, using NSCs collected from young rats, demonstrated that reducing the oxygen tension during culture in the laboratory from 20 percent to 3 percent resulted in significant cell death, while maintaining a 3 percent level protected them.

They saw similar results when they transplanted the stem cells into the brains of adult rats.

“NSCs cultured at an oxygen level of 3 percent rather than 20 percent oxygen approximately doubled survival in the immediate post-transplantation phase,” Dr. Stacpoole reported.

In addition, the low oxygen tensions resulted in more cells developing into oligodendrocytes, both in vitro and in vivo. Oligodendrocytes are a type of brain cell. While considered the most vulnerable cells in the central nervous system, they also are among the most important as they produce the insulating sheet that protects nerve fibers.

“While cell transplantation strategies hold promise for the treatment of a wide range of human diseases, it is known that many transplanted cells die within the first few days,” said Anthony Atala, M.D., editor of STEM CELLS Translational Medicine and director of the Wake Forest Institute for Regenerative Medicine. “This study demonstrates that oxygen in the cell culture environment is an important consideration when preparing cells for transplantation.”

###

The full article, “NPCs cultured at physiologically relevant oxygen tensions have a survival advantage following transplantation,” can be accessed at http://www.stemcellstm.com.

About STEM CELLS Translational Medicine: STEM CELLS TRANSLATIONAL MEDICINE (SCTM), published by AlphaMed Press, is a monthly peer-reviewed publication dedicated to significantly advancing the clinical utilization of stem cell molecular and cellular biology. By bridging stem cell research and clinical trials, SCTM will help move applications of these critical investigations closer to accepted best practices.

About AlphaMed Press: Established in 1983, AlphaMed Press with offices in Durham, NC, San Francisco, CA, and Belfast, Northern Ireland, publishes two other internationally renowned peer-reviewed journals: STEM CELLS® (http://www.StemCells.com), celebrating its 31th anniversary in 2012, is the world's first journal devoted to this fast paced field of research. The Oncologist® (http://www.TheOncologist.com), also a monthly peer-reviewed publication, entering its 18th year, is devoted to community and hospital-based oncologists and physicians entrusted with cancer patient care. All three journals are premier periodicals with globally recognized editorial board dedicated to advancing knowledge and education in their focused disciplines.

Read the full story at http://www.prweb.com/releases/2013/5/prweb10737667.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related medicine news :

1. Race, Income Tied to Breast Cancer Treatment Delays, Reduced Survival
2. Childhood Meningitis Tied to Reduced Education, Employment Prospects
3. Swedish study suggests reduced risk of dementia
4. Reduced Fine-Tuning of Brain May Hamper Face Recognition in Autism
5. RI Hospital: Radiation can be reduced while maintaining high quality in CT colonography
6. Study shows reduced risk of preterm birth for pregnant women vaccinated during pandemic flu
7. Study suggests reduced lung function in infancy associated with wheeze later
8. Exercise linked with reduced prostate cancer risk in Caucasians but not African-Americans
9. Exercise linked with reduced prostate cancer risk in Caucasians but not African Americans
10. Amputations among people with diabetes can be reduced by 50 percent
11. Spread of Cancer May be Reduced by Maximizing Melatonin by Blocking Blue Light Says Photonic Developments LLC
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/6/2016)... ... February 06, 2016 , ... Shark Finds and ... launch of a new DRTV campaign with Belly Bands. , Having a dog is ... sprays to puppy pads and find nothing works, get Belly Bands, the easiest ...
(Date:2/5/2016)... Bethpage, NY (PRWEB) , ... February 05, 2016 , ... ... florist-quality long-stem roses in a variety of colors, assortments and packaging. This staple for ... at any King Kullen location. , For Valentine’s Day, not only are long-stem ...
(Date:2/5/2016)... ... February 05, 2016 , ... Successful recruitment and retention efforts, ... scientific initiatives have all marked the last 12 months at Roswell Park Cancer ... the nation’s oldest cancer center, Candace S. Johnson, PhD, outlined the many accomplishments ...
(Date:2/5/2016)... ... 05, 2016 , ... Francisco Canales, MD and Heather Furnas, ... Valley office. The technique utilizes the body’s own healing abilities to quickly rejuvenate ... are part of only a select few cosmetic surgeons bringing this procedure to ...
(Date:2/5/2016)... (PRWEB) , ... February 05, 2016 , ... After years ... General Hospital Burn Unit, plastic and cosmetic surgeon Dr. Wayne Carman transitioned to chief ... Hospital. He successfully completed his first three-year term as chief and began a second ...
Breaking Medicine News(10 mins):
(Date:2/4/2016)... , Feb. 4, 2016 Worldwide ... achieve significant growth as next generation systems provide ... use radiology for cancer surgery. New systems pinpoint ... overdosing that has been such a problem previously, ... delivered. Radiosurgery robots take cancer surgery far beyond ...
(Date:2/4/2016)... 4, 2016 Summary Breast cancer, a ... the most common cancer in women worldwide, accounting for ... prevalent. The number of women diagnosed with breast cancer ... number of deaths has declined due to earlier diagnosis ... been revolutionized in the past four decades, especially with ...
(Date:2/4/2016)... DIEGO, Feb. 4, 2016  Aethlon Medical, Inc. ... affinity biofiltration devices to treat life-threatening diseases, today ... 2016 ended December 31, 2015. ... objectives set forth in our last quarterly call, ... reinforce our long-term objective to establish the Aethlon ...
Breaking Medicine Technology: