Navigation Links
Recipe for cell reprogramming adds protein
Date:8/6/2008

CAMBRIDGE, Mass. (August 6, 2008) A drug-like molecule called Wnt can be substituted for the cancer gene c-Myc, one of four genes added to adult cells to reprogram them to an embryonic-stem-cell-like state, according to Whitehead researchers. Researchers hope that such embryonic stem-cell-like cells, known as induced pluripotent (IPS) cells, eventually may treat diseases such as Parkinson's disease and diabetes.

Demonstrated in mice, the elimination of c-Myc represents an important step in creating IPS cells in a manner that in the future may be applied to human therapeutics.

"This is a good sign for the possible replacement of the other three genes used to reprogram cells," says Ruth Foreman, a MD/PhD student in the lab of Whitehead Member Rudolf Jaenisch and a lead co-author on the paper, published online in Cell Stem Cell on August 6. The other lead co-authors are Alex Marson, an MD/PhD student in the labs of Jaenisch and Whitehead Member Richard Young, and Brett Chevalier, a research scientist in the Young lab.

"IPS cells hold great potential for future medicine, but we must learn how to generate these cells in a manner that is safe for clinical therapies," says Young, who is also a professor of biology at Massachusetts Institute of Technology. "This advance in reprogramming is one key step toward that goal,"

Currently, IPS cells can be created by reprogramming adult cells through the use of viruses to transfer four genes (Oct4, Sox2, c-Myc and Klf4) into the cells' DNA. The activated genes then override the adult state and convert the cells to embryonic-like IPS cells.

However, this method poses significant risks for potential use in humans.

First, the viruses employed in the process, called retroviruses, are associated with cancer because they insert DNA anywhere in a cell's genome, thereby potentially triggering the expression of cancer-causing genes, or oncogenes. Second, c-Myc is a known oncogene whose overexpression can also cause cancer. For IPS cells to be employed to treat human diseases such as Parkinson's, researchers must find safe alternatives to reprogramming with retroviruses and oncogenes.

Earlier research has shown that c-Myc is not strictly required for the generation of IPS cells. However, its absence makes the reprogramming process time-consuming and highly inefficient.

To bypass these obstacles, the Whitehead researchers replaced c-Myc and its retrovirus with a naturally occurring signaling molecule called Wnt3a. When added to the fluid surrounding the cells being reprogrammed, Wnt3a promotes the conversion of adult cells into IPS cells.

"We're not sure if the Wnt molecule is doing the same thing as c-Myc or complementing c-Myc's activity," says Chevalier. "But it does increase stem cell growth similar to c-Myc."

"This is a good start toward using external cues instead of genetic manipulation to reprogram cells," says Marson. "But we still need to eliminate the need for retroviruses for the three other genes."

Although the technique is promising in mouse cells, its potential applications in humans have not been studied, emphasizes Jaenisch, who is also a professor of biology at MIT. "Is the same pathway acting in the human system and can Wnt molecules be used to reprogram human cells?" he asks. "We don't know, but I think those are very important questions to investigate."


'/>"/>

Contact: Cristin Carr
carr@wi.mit.edu
617-324-0460
Whitehead Institute for Biomedical Research
Source:Eurekalert

Related medicine news :

1. Whole Foods Market(R) Launches The Real Deal Program With Budget Recipes, Real Steals and Smart Shopping Advice
2. Thirsting for Healthful Smoothie Recipes That got milk?
3. dLife Launches the Worlds Largest and Most Comprehensive Online Food and Diabetic Recipe Search Tool
4. Personalized Plates for Picky Palates: Moms can Satisfy all With One Recipe, Four Ways
5. MultiVu Video Feed: LIFE AND STYLE EXPERT SANDRA LEE OFFERS RECIPES AND SHORTCUTS IN THE KITCHEN TO PEOPLE WITH RHEUMATOID ARTHRITIS
6. Celebrity Chef Sandra Lee Offers Recipes and Shortcuts in the Kitchen to People with Rheumatoid Arthritis
7. Florida Orange Juice Adds a Healthy Splash of Flavor and Sunshine to Recipes
8. Idaho Potato Commission Launches Recipes for Relief in Celebration of the International Year of the Potato
9. Genghis Grill Offers Recipes Featuring Heart Healthy Dining Options
10. AUDIO from Medialink and Prilosec OTC: Recipes for Heartburn-Friendly Super Bowl Snacking
11. Holiday Travel, Bad Eating Habits a Recipe for Stomach Trouble
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/16/2017)... ... August 16, 2017 , ... QualDerm Partners , a ... today announced Cumberland Skin Surgery and Dermatology has joined the company ... in both Hermitage and Lebanon, Tennessee, provides comprehensive general dermatology and skin cancer ...
(Date:8/16/2017)... Island, NY (PRWEB) , ... August 16, 2017 , ... ... with IV starts and blood draws. By broadly deploying AccuVein devices, Maury Regional Medical ... takes much of the guesswork out of a needle stick and more importantly, helps ...
(Date:8/16/2017)... ... August 16, 2017 , ... Paul Vitenas, MD, FACS , is honored ... The annual list identifies the nation’s top physicians, in a variety of specialties. This ... the top of Castle Connolly’s coveted ranking. , Castle Connolly is the nation’s trusted ...
(Date:8/16/2017)... ... , ... A global leader in the treatment of clubfoot, CURE International ... states—bringing the country one step closer to eliminating clubfoot as a lifelong disability by ... track to enroll 10,000 children in the clubfoot treatment program in this year alone. ...
(Date:8/16/2017)... , ... August 16, 2017 , ... ... states: “Drying the endoscope after every reprocessing cycle, both between patient procedures and ... and nosocomial infections. Drying is as important to the prevention of disease transmission ...
Breaking Medicine News(10 mins):
(Date:8/1/2017)... CerSci Therapeutics , a non-opioid drug development firm based ... notice from the National Institute on Drug Abuse (NIDA) ... has been awarded a Direct-to-Phase II Small Business Innovation ... an additional $1,000,000 to follow in 2018. These funds ... lead non-opioid drug candidate CT-044 to the Food and ...
(Date:7/28/2017)... 2017 EnvoyHealth, a Diplomat company, has partnered with ... CleverCap LITE, a technology designed to improve patient medication ... health technology solutions and services that help track and ... CleverCap LITE offers medication monitoring and control for ... Records date and time ...
(Date:7/26/2017)... , July 26, 2017 Sancilio Pharmaceuticals Company, ... our clinical trial evaluating Altemia TM , an oral ... and Sickle Cell Disease (SCD). The SCOT Trial, is ... efficacy and safety of Altemia TM in pediatric ... under US IND 125274. ...
Breaking Medicine Technology: