Navigation Links
Recently discovered virus associated with pediatric respiratory tract infection in Germany
Date:11/20/2007

November 20, 2007Using a rapid, sensitive, and inexpensive diagnostic tool called MassTag PCR, scientists at Columbia University Mailman School of Public Healths Center for Infection and Immunity implicated a new human rhinovirus as the cause of severe pediatric respiratory tract infections in Europe. Their findings are published in the December 15 issue of the Journal of Infectious Diseases (currently available online).

The research team used MassTag PCR to investigate 97 samples, collected over a three-year period, from children with hospital-admitted, acute respiratory illness wherein no pathogen was identified through routine laboratory testing. Human rhinoviruses were the most frequent viruses detected in the sample set representing 75% of the identified viruses.

Human rhinoviruses are frequent causes of respiratory illness worldwide. Although they are most commonly associated with self-limited upper respiratory tract disease, lower respiratory tract infections related to HRV are being increasingly reported in infants, elderly persons, and immunocompromised patients. HRVs are also implicated in exacerbations of asthma, chronic bronchitis, and acute bronchiolitis.

Acute respiratory infection is a significant cause of morbidity and mortality in children worldwide. Accurate identification of causative agents is critical to case management and to prioritization in vaccine development, stated W. Ian Lipkin, MD, professor of Epidemiology, Neurology, and Pathology at Columbia University, director of the Center for Infection and Immunity at the Mailman School of Public Health, and senior author of the paper.

In up to 50% of cases of severe respiratory disease, a causative agent is not identified, despite the application of PCR assays as well as classical diagnostic methods including culture, antigen tests, and serology. Broad-range molecular systems pioneered by this team including MassTag PCR, GreeneChips and high throughput metagenomic sequencing, enable pathogen discovery, surveillance and medical diagnostics. Recent application of these technologies led to diagnosis of viral hemorrhagic fevers in Africa, a new virus causing transplant deaths, and detection of Israel Acute Paralysis Virus in honey bees with Colony Collapse Disorder.

To detect pathogens, MassTag PCR uses small molecular tags to detect up to 30 different pathogens simultaneously in one test. Genetic material from a throat swab or other sample is extracted and then mixed with PCR primersshort pieces of DNA that recognize specific nucleic acid sequences within the genomes of the target viruses or bacteria. If a throat swab contains pathogens with nucleic acid sequences that match those of the primers, then the primers will copy the target DNA several million times. Likewise the molecular tags, different in mass for each of the primers, are also amplified making them easily detectable by mass spectrometry, a technology that identifies molecules based on their masses.

The results of the study confirm our earlier findings in New York, namely, that these viruses represent a clinically significant but previously unappreciated species within the entero-/rhinoviruses, one of the longest known and most intensely studied virus groups, stated Thomas Briese, PhD, associate professor of clinical Epidemiology, who coordinated the study. We urgently need drugs and vaccines to address the challenges they pose to child health.

In an editorial commentary in the December 15 issue of the Journal of Infectious Diseases, Anne Moscona, MD, departments of Pediatrics and of Microbiology and Immunology, Weill Medical College of Cornell University, states that the work of Dr. Lipkins team with MassTag PCR, provides a paradigm for new detection strategies for early recognition and containment of a wide range of respiratory pathogens.


'/>"/>

Contact: Randee Sacks Levine
rs363@columbia.edu
212-305-8044
Columbia University's Mailman School of Public Health
Source:Eurekalert

Related medicine news :

1. First biomarker discovered that predicts prostate cancer outcome
2. New prion protein discovered by Canadian scientists may offer insight into mad cow disease
3. New genetic lineage of Ebola virus discovered in great apes
4. Ear infection superbug discovered to be resistant to all pediatric antibiotics
5. New peritoneal dialysis diagnostic discovered
6. New peptide communication factor enabling bacteria to talk to each other discovered
7. Key to MRSA Virulence in Community Discovered
8. Virus Could Help Drive Obesity
9. Scientists detect presence of marburg virus in african fruit bats
10. Norwalk virus: Cruise ship illness challenging and costly to hospitals, too
11. Human Papilloma Virus vaccines may decrease chances of oral cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... ... October 13, 2017 , ... Coveros, a ... has been awarded a contract by the Center for Medicare and Medicaid Services ... accelerate the enterprise use of Agile methodologies in a consistent and high value ...
(Date:10/12/2017)... ... October 12, 2017 , ... First Healthcare Compliance ... management, will showcase a range of technology and learning solutions at the 68th ... and Expo to be held October 14–18, 2017 at the Mandalay Bay Resort ...
(Date:10/12/2017)... ... October 12, 2017 , ... The company has developed ... consumer and regulatory authorities worldwide. From Children’s to Adults 50+, every formula has ... highest standard. , These products are also: Gluten Free, Non-GMO, Vegan, Soy ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... leader in post-acute health care, have expanded their existing home health joint venture ... , AccentCare has been operating a joint venture home health company with Asante, ...
(Date:10/12/2017)... ... ... HMP , a leader in healthcare events and education, today announced that ... ‘Best B-to-B Healthcare Website.’ Winners were announced during the Eddie & Ozzie Awards luncheon ... editorial and design excellence across a range of sectors. This year’s program included the ...
Breaking Medicine News(10 mins):
(Date:10/10/2017)... , Oct. 10, 2017   West Pharmaceutical ... innovative solutions for injectable drug administration, today shared the ... ID Adapter for improving the intradermal administration of polio ... Skin Vaccination Summit in May 2017 by Dr. ... Polio Department, World Health Organization (WHO), and recently published ...
(Date:10/4/2017)... 2017 OBP Medical , a ... devices, today announced regulatory approval from ... Agência Nacional de Vigilância Sanitária (ANVISA)) to market ... retractor with integrated LED light source and smoke ... exposure of a tissue pocket or cavity during ...
(Date:10/2/2017)... 2017 The Rebound mobile app is poised to ... the tide of prescription drug addiction. The app empowers users ... and stepping down their dosage in a safe, controlled manner ... 2017; the first 100,000 people to sign up will enjoy ... ...
Breaking Medicine Technology: