Navigation Links
Quest for designer bacteria uncovers a 'Spy'
Date:2/14/2011

ANN ARBOR, Mich.---Scientists have discovered a molecular assistant called Spy that helps bacteria excel at producing proteins for medical and industrial purposes.

Bacteria are widely used to manufacture proteins used in medicine and industry, but the bugs often bungle the job. Many proteins fall apart and get cut up inside the bacteria before they can be harvested. Others collapse into useless tangles instead of folding properly, as they must in order to function normally.

A research team led by James Bardwell, who is a professor of molecular, cellular and developmental biology and of biological chemistry, as well as a Howard Hughes Medical Institute investigator, at the University of Michigan, developed a way to coerce bacteria into making large quantities of stable, functional proteins. Then, in exploring why these designer bacteria were so successful, the scientists discovered the molecular helper, Spy.

The research is scheduled for online publication Feb.13 in the journal Nature Structural & Molecular Biology.

In the first phase of the research, the team designed biosensors that directly link protein stability to the antibiotic resistance of bacteria. When a poorly folded, unstable protein is inserted into the middle of the biosensor in a bacterium, it disrupts the bug's resistance to antibiotics. When the protein is stabilized, resistance is restored.

The researchers inserted a particularly unstable protein into Escherichia coli (E. coli), which forced the bacteria to either adapt by improving protein stability or die when exposed to antibiotics. Through a "directed evolution" experiment, in which the scientists selected colonies with increasing antibiotic resistance---and increasing protein stabilitythe team generated designer bacteria that produced up to 700 times more of the previously unstable protein.

"It is exciting to realize that if even bacteria are asked in the right way, they can come up with good solutions to hard problems," said postdoctoral fellow Shu Quan, who spearheaded the work.

In looking to see why the designer bacteria were so much better at producing proteins, the scientists found that the efficient microbes were making much more of a small protein called Spy. Further study showed that the cradle-shaped Spy aids in protein refolding and protects unstable proteins from being cut up or sticking to other proteins.

"Our work may usher in an era of designer bacteria that have had their folding environment customized so that they can now efficiently fold normally unstable proteins," Bardwell said.


'/>"/>

Contact: Nancy Ross-Flanigan
rossflan@umich.edu
734-647-1853
University of Michigan
Source:Eurekalert  

Related medicine news :

1. University of Virginia Health System Medical Laboratories Selects Sunquest's Specimen Collection Solution
2. Sunquest Announces Major Software Updates and Enhancements
3. Anthem Blue Cross of California Agrees to Request From California Department of Insurance for Additional Time to Re-Review Rate Increase
4. GenomeQuest Hosts Seminar Focused On Web-based Searching for Patent Information Across Global Sequence Databases
5. Sunquest Introduces Integrated Clinical Environment Physician Portal Solution in US
6. Sunquest Completes System-Wide Implementation at Cleveland Clinic Hospital Laboratories
7. QuestNet and AFC: Raising the Bar of Asian Football
8. Chronic Fatigue Syndrome-Virus Link Questioned
9. Napa State Hospital Doctors Question DMH Judgment
10. Study Questions Value of Test for Peripheral Artery Disease
11. Testimony Before the House Committee on Foreign Affairs Regarding the Fiscal Year 2011 Budget Request
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Quest for designer bacteria uncovers a 'Spy'
(Date:4/25/2017)... ... 25, 2017 , ... The doctors at Wall Centre Dental are now expanding ... and require sedation to receive dental care. The doctors offer three treatment options to ... cleanings to oral surgery, at their dental office in Vancouver. Wall Centre Dental is ...
(Date:4/25/2017)... ... April 25, 2017 , ... Patients ... that achieves results in a fraction of the time as traditional braces – ... Significance Dental Specialists, now offers this revolutionary treatment with or without a referral. ...
(Date:4/25/2017)... ... April 25, 2017 , ... As Spring reignites vigilance ... research related to Zika virus during pregnancy, as well as other prenatal exposures ... science. , The Teratology Society is an international and multidisciplinary ...
(Date:4/25/2017)... (PRWEB) , ... April 25, 2017 , ... Buyers and ... recreational users to dispensaries and head shops –can’t help but be heartened by the ... the tell-tale cannabis odor aptly described as “skunk smell.” At last they can ...
(Date:4/25/2017)... Clara, CA (PRWEB) , ... April 25, 2017 ... ... announces the addition of predictive analytics to its patient care management module. Using ... compliance even before a patient has been initiated on continuous positive airway pressure ...
Breaking Medicine News(10 mins):
(Date:4/18/2017)... , April 18, 2017  Cardinal Health (NYSE: ... 1 fiscal 2017 earnings per share (EPS) guidance ... 2019.  This is in conjunction with this morning,s announcement ... Vein Thrombosis and Nutritional Insufficiency businesses. Cardinal ... continuing operations will be at the bottom of its ...
(Date:4/18/2017)... , April 18, 2017 Viverae ® , ... announce the integration of IBM ® Watson Campaign ... deliver targeted communications for a personalized experience. Through digital ... on their health in real time. The enhanced experience ... most to members, wherever they are in their journey ...
(Date:4/18/2017)... -- Astute Medical, Inc., developer of biomarkers for better healthcare, ... the 2017 National Kidney Foundation (NKF) Spring Clinical ... 22. Physicians will present data on two biomarkers, ... acute kidney injury (AKI) during the management of patients ... Elevated levels of TIMP-2 and IGFBP-7 have been shown ...
Breaking Medicine Technology: