Researchers at Washington University School of Medicine in St. Louis have learned why changes in a single gene, ROP18, contribute substantially to dangerous forms of the parasite Toxoplasma gondii. The answer has likely moved science a step closer to new ways to beat Toxoplasma and many other parasites.
In a study published in Cell Host & Microbe, scientists show that the ROP18 protein disables host cell proteins that would otherwise pop a protective bubble the parasite makes for itself. The parasite puts the bubble on like a spacesuit by forming a membrane around itself when it enters host cells. This protects it from the hostile environment inside the cell, which would otherwise kill it.
"If we can find therapies that block ROP18 and other parasite proteins like it, that could give the host the upper hand in the battle against infection," says first author Sarah Fentress, a graduate student in the laboratory of L. David Sibley, PhD, professor of molecular microbiology.
Infection with Toxoplasma, or toxoplasmosis, is most familiar to the general public from the recommendation that pregnant women avoid changing cat litter. Cats are commonly infected with the parasite, as are some livestock and wildlife.
"The exact role of ROP18 and related proteins in human disease remains to be studied," says Sibley. "But mice are natural hosts of Toxoplasma, so studies in laboratory mice are relevant to the spread of infection."
Epidemiologists estimate that as many as one in every four humans is infected with Toxoplasma. Infections typically cause serious disease only in patients with weakened immune systems. In some rare cases, though, infection in patients with healthy immune systems leads to serious eye or central nervous system disease, or congenital defects or death in the fetuses of pregnant women.
In the new study, Fentress showed that the ROP18 protein binds to a class
|Contact: Michael C. Purdy|
Washington University School of Medicine