Navigation Links
Potent, puzzling and (now less) toxic: Team discovers how antifungal drug works
Date:4/15/2014

le is one of the most challenging to work with because it has a very complex structure, is poorly soluble and is sensitive to light, oxygen and acid," Burke said.

Amphotericin also interacts with membranes, which are notoriously difficult to study. Many labs, including Burke's, have tried to figure out the three-dimensional structure of amphotericin by crystallizing it, a standard technique. So far, all attempts have failed.

So the team turned to one of the most powerful tools for studying non-crystalline samples: solid-state nuclear magnetic resonance (NMR) spectroscopy, which measures how atomic nuclei respond to changes in a magnetic field.

"NMR is a great technique for detecting signals and interpreting the structural properties of molecules in solution," said Rienstra, who led the NMR work with graduate students Mary Clay and Tom Anderson. But few labs use it to track interactions between small molecules in membranes, he said. "We developed several new NMR experiments to study amphotericin in the presence of membranes."

Previous studies had found evidence that amphotericin opens up ion channels in membranes, perhaps making them more leaky to charged atoms that could disrupt a cell. Most scientists assumed that this was the drug's main mode of action.

But the evidence also suggested that amphotericin interacted with sterols, such as cholesterol in animal cells and ergosterol in yeast. Rienstra and Burke focused on amphotericin's influence on sterols, hypothesizing that this might be a key to its toxicity.

The initial NMR data supported this idea, indicating that very little of the drug less than 5 percent actually formed channels in membranes. Using NMR and other experimental tools, the Rienstra/Burke team found that most of the amphotericin aggregates on the exterior of membranes, extracting sterols out of membranes like a sponge. Cell death follows soon after.

"For over 50 years, the mechanis
'/>"/>

Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3

Related medicine news :

1. Global Stem Cells Market (Adult, Human Embryonic , Induced Pluripotent, Rat-Neural etc)- Industry Analysis, Size, Share, Growth, Trends and Forecast, 2012 - 2018
2. IBN discovers human neural stem cells with tumor targeting ability
3. Rutgers team discovers novel approach to stimulate immune cells
4. UZH research team discovers the origins of key immune cells
5. Yale team discovers how stress and depression can shrink the brain
6. Mayo, UCSF team discovers genomic variant that increases risk of brain tumors
7. Penn-Temple team discovers gatekeeper for maintaining health of cell energy source
8. Aldrich Materials Science discovers liquid-free preparation of metal organic frameworks
9. U-M sibling study discovers genetic region linked to control of key blood-clotting protein
10. Kwiksure Discovers Uses and Limitations of Vehicle GPS in Hong Kong
11. Its the way you tell em: Study discovers how the brain controls accents and impersonations
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Potent, puzzling and (now less) toxic: Team discovers how antifungal drug works
(Date:12/26/2014)... 26, 2014 Parker & Sons, ... best in heating, cooling and plumbing contractor services ... emergency services in 2014 with regard to air-conditioning ... Arizona is known for its incredibly hot summers. ... the intricacies and details of a wide variety ...
(Date:12/25/2014)... TX (PRWEB) December 26, 2014 The ... to 2023” focuses on the current treatment landscape, unmet ... cancer market. TS-1 is an anti-cancer drug which is ... orally and is also used for treating gastric cancer ... and otastat potassium. The drug was first approved in ...
(Date:12/25/2014)... 2014 The short film “Color of Honor” ... honor of a true 9/11 hero, Welles Remy Crowther. It ... the 24 hours of Nuremberg International Short Film Festival and ... Actress, screenwriter and director Luciana Lagana plays the ... with her husband, Gregory Graham - Graham plays ...
(Date:12/25/2014)... December 25, 2014 Recently, LunaDress.co.uk has updated its ... and related accessories. Now, all of the models at LunaDress.co.uk come ... a groom lifts the wedding veil can turn out to be ... charm. A wedding veil can always bring surprise to a wedding. ... on how to choose veils for a big day . ...
(Date:12/25/2014)... Dennis Thompson HealthDay Reporter ... could be closing in on a "fountain of youth" drug ... health of older adults, a new study suggests. Seniors ... a drug that targets a genetic signaling pathway linked to ... report. The experimental medication, a version of ...
Breaking Medicine News(10 mins):Health News:Parker & Sons Announces Record Demand For 24 Hour Emergency Services In 2014 With Regard To Air-Conditioning Systems 2Health News:Parker & Sons Announces Record Demand For 24 Hour Emergency Services In 2014 With Regard To Air-Conditioning Systems 3Health News:TS-1 Colorectal Cancer Market Analysis and Forecast to 2023 Report at RnRMarketReserach.com 2Health News:TS-1 Colorectal Cancer Market Analysis and Forecast to 2023 Report at RnRMarketReserach.com 3Health News:The True 9/11 Hero Story “Color of Honor” Premieres in Germany and Bangladesh: Luciana Lagana Plays the Mother of the Lead and Co-Produces with Blaise Dolcemaschio. 2Health News:The True 9/11 Hero Story “Color of Honor” Premieres in Germany and Bangladesh: Luciana Lagana Plays the Mother of the Lead and Co-Produces with Blaise Dolcemaschio. 3Health News:LunaDress Provides Professional Tips On Shopping Wedding And Prom Dresses 2Health News:Researchers Take 'First Baby Step' Toward Anti-Aging Drug 2Health News:Researchers Take 'First Baby Step' Toward Anti-Aging Drug 3
... Netherlands, May 8 LyondellBasell Industries,has completed ... of its management systems at its plants ... MonoPropyleneGlycol USP/EP,(MPG-USP/EP) is produced., HACCP is ... preventing,food safety hazards. The comprehensive nature of ...
... 8 PennWell Corporation is launching a,third dental ... -,7th, 2008. Registration is now open and available ... use,leading-edge technology to bring dental professionals together with,colleagues ... computer., "DDS Tech Fair is designed to ...
... Academies Keck FUTURES INITIATIVE announced today the recipients of ... research on aging and healthspan -- the period of ... The 15 projects chosen represent a wide range ... of the fifth annual FUTURES conference, "The Future of ...
... physicians across the country who lead small practices are ... FACP, president of the American College of Physicians (ACP) ... Dr. Dale emphasized that practices are medicines small businesses, ... Medicares flawed reimbursement rates and formulas. , Approximately 20 ...
... non-profit youth,development organization, has been awarded a $25,000 grant ... launched Healthy Communities Program,launched today in conjunction with District ... General Mills Foundation and the Mayor,s Commission,on Food and ... to focus on,helping children eat healthy and stay physically ...
... people discover mold on their bread, they immediately throw ... the tiny fungus. A University of Missouri scientist, along ... mechanism in the reproductive cycle of a certain species ... abnormalities by silencing unmatched genes during meiosis (sexual reproduction). ...
Cached Medicine News:Health News:Raising the Bar on MonoPropyleneGlycol USP/EP 2Health News:PennWell Announces DDS Tech Fair 2Health News:Keck Futures Initiative awards $1M for 15 research projects 2Health News:Keck Futures Initiative awards $1M for 15 research projects 3Health News:Keck Futures Initiative awards $1M for 15 research projects 4Health News:Keck Futures Initiative awards $1M for 15 research projects 5Health News:Keck Futures Initiative awards $1M for 15 research projects 6Health News:ACP says Medicare cuts will hurt physicians in small practices 2Health News:Brainfood Awarded $25,000 Grant from General Mills Foundation through DC Mayor Fenty's New Health Communities Program 2Health News:Bread mold may hold secret to eliminating disease-causing genes 2
(Date:12/24/2014)... , Dec. 24, 2014  Commonwealth Cornerstone Group (CCG) today ... Credits transaction on Tuesday to help fund the expansion and ... in Sharon . PHN ... Pennsylvania . It is the largest employer in ... than 150 people on staff. Currently, PHN Sharon ...
(Date:12/24/2014)... 2014  Conkwest, Inc., the Natural Killer Cell ... Killer (NK) cell-line platform, Neukoplast® (NK-92™) as an ... , NantWorks founder, physician scientist and biotechnology entrepreneur, ... approximately $48 million of the Company,s Class A ... will be named Co-Chairman of the Conkwest Board ...
(Date:12/24/2014)... 23, 2014 Echo Therapeutics, Inc. (NASDAQ: ... focused on skin permeation, continuous glucose monitoring and ... Hollander has been appointed Chief Executive Officer ... than 20 years of experience in the medical ... recently served as Vice President, Business Development, Otsuka ...
Breaking Medicine Technology:Commonwealth Cornerstone Group Announces $8 Million in Tax Credits to Expand and Consolidate Healthcare Services in Sharon 2Commonwealth Cornerstone Group Announces $8 Million in Tax Credits to Expand and Consolidate Healthcare Services in Sharon 3Conkwest Announces $50m In Strategic Investments And Appoints NantWorks Founder, Dr. Patrick Soon-Shiong As Co-Chairman Of The Board 2Conkwest Announces $50m In Strategic Investments And Appoints NantWorks Founder, Dr. Patrick Soon-Shiong As Co-Chairman Of The Board 3Conkwest Announces $50m In Strategic Investments And Appoints NantWorks Founder, Dr. Patrick Soon-Shiong As Co-Chairman Of The Board 4Conkwest Announces $50m In Strategic Investments And Appoints NantWorks Founder, Dr. Patrick Soon-Shiong As Co-Chairman Of The Board 5Echo Therapeutics Announces Scott W. Hollander as President & Chief Executive Officer 2Echo Therapeutics Announces Scott W. Hollander as President & Chief Executive Officer 3
... Technology Co., Ltd, one of the leading manufacturers of medical ... machine. Noble Laser,s IPL machine always enjoys a ... world choose this machine because of high configuration, stable quality ... free choosing. Please log in to www.noblelaser.com to ...
...  The Preserve Community Pharmacy Access NOW! (PCPAN) coalition today applauded ... the proposed merger between Express Scripts, Inc. (ESI) and ... In a letter to the Federal Trade Commission (FTC), ... 100,000 active and retired union mine workers. ...
Cached Medicine Technology:Beijing Noble Laser Technology Co., Ltd Offers a Great Deal on its IPL Machine 2Nationwide Coalition Applauds United Mine Workers For Voicing Concern Over Proposed ESI/Medco Merger 2
... national reference laboratory and a worldwide leader ... offers an extensive test menu of highly ... and anatomic pathology. Owned by the University ... than half of the nation's university teaching ...
... national reference laboratory and a worldwide leader ... offers an extensive test menu of highly ... and anatomic pathology. Owned by the University ... than half of the nation's university teaching ...
... is a national reference laboratory and a ... development. ARUP offers an extensive test menu ... in clinical and anatomic pathology. Owned by ... include more than half of the nation's ...
... a national reference laboratory and a worldwide ... ARUP offers an extensive test menu of ... clinical and anatomic pathology. Owned by the ... more than half of the nation's university ...
Medicine Products: