Navigation Links
Penn researchers show nuclear stiffness keeps stem cells and cancer cells in place
Date:2/25/2014

th lead author Takamasa Harada, a graduate student in his lab, conducted the studies with fellow lab members Joe Swift, Jerome Irianto, Jae-Won Shin, Kyle Spinler, Avathamsa Athirasala, Dave Dingal and Irena Ivanovska, as well as undergraduate student Rocky Diegmiller.

The study's experiments were conducted on immortalized human cancer cells as well as human-donor-derived mesenchymal stem cells, which are in wide use in clinical trials for tissue repair. The researchers either inhibited or overexpressed lamin-A in the cells, then placed both kinds on top of a thin sheet with very small pores. By adding blood serum to a chamber on the bottom of the porous sheet, the researchers encouraged the cells to push, pull and squeeze their nuclei through the pores.

Looking under a microscope at the cells that made it though the sheet revealed very few of the cells where lamin-A had been overexpressed. There was also a dearth of cells where lamin-A was strongly repressed. The cells that were most successful in migrating through the sheet's pores were the ones with lamin-A only slightly less than normal.

"The decreased migration with very low lamin-A levels was especially surprising," Harada said, "and so we measured the physical stiffness of the various nuclei, confirming that cell nuclei were systematically softer with low levels of lamin-A."

"While cells with stiffer nuclei are clearly unable to push or pull their nuclei through the pores," he said, "all of the softer nuclei could be moved through more easily, which presented a paradox."

To resolve the paradoxical decrease in cell number with very soft nuclei, the researchers saw that the cells on the bottom of the porous sheet turned on a marker for cell death that was otherwise absent from cells on top and the lower the lamin-A the greater the number of dying cells. As a cell squeezes its nucleus through a small pore, cell death is triggered and more so when lamin-A is low. '/>"/>

Contact: Evan Lerner
elerner@upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert  

Page: 1 2 3

Related medicine news :

1. CWRU researchers find byproducts of bacteria-causing gum disease incite oral cancer growth
2. Researchers make the invisible visible
3. Researchers look for culprit behind oral health problems in HIV-positive patients
4. Clemson researchers develop sticky nanoparticles to fight heart disease
5. NUS researchers make new discovery of protein as a promising target for treatment of ATC
6. Scripps researchers recommend mobile compression device to prevent DVT after joint surgery
7. No such thing as porn addiction, researchers say
8. LA BioMed researchers report on promising new therapy for devastating genetic disorder
9. NIH-funded researchers use antibody treatment to protect humanized mice from HIV
10. Researchers blend orthopedics, engineering to better repair torn rotator cuffs
11. Researchers discover new hormone receptors to target when treating breast cancer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Penn researchers show nuclear stiffness keeps stem cells and cancer cells in place
(Date:8/22/2014)... Aug. 22, 2014 (HealthDay News) -- Many Americans know little ... the possibility of an outbreak in the United States, a ... they are worried that there will be a major Ebola ... they or an immediate family member will get sick with ... latest Harvard School of Public Health poll. However, those ...
(Date:8/22/2014)... York (PRWEB) August 22, 2014 ... morcellators ( http://www.injurybeacon.com/power-morcellator/ ) in uterine surgeries continues ... Massachusetts insurance companies have decided to limit coverage ... the Worcester Telegram, Blue Cross-Blue Shield of Massachusetts ... surgeries that involve morcellation as of September 1, ...
(Date:8/22/2014)... Millennium Treatment Group, a drug ... recently launched a new website. Patients, parents, loved ... now find Millennium Treatment Group at http://www.millenniumtreatmentgroup.com ... at the beginning of July. Everything anyone would ... found on the website. There’s an About Us ...
(Date:8/22/2014)... Vegas, NV (PRWEB) August 22, 2014 ... by Kevin Richardson to restore his own vision from legally ... Shane Michaels, prompting an investigative review. , “There is ... glasses or contacts for everyday function, and most people just ... resort to corrective eye surgery that can be pretty risky ...
(Date:8/22/2014)... Beat Eczema , Susan Clark’s best-selling ... methods to completely cure the eczema symptoms they have ... Michaels, prompting an investigative review. , “Although eczema ... can still be extremely debilitating and life-altering for those ... and pain that it causes on the skin can ...
Breaking Medicine News(10 mins):Health News:Many Americans Harbor Unfounded Fears About Ebola Outbreak: Survey 2Health News:As Power Morcellator Controversy Grows, Bernstein Liebhard LLP Comments on Massachusetts Insurers’ Plans to Limit Coverage of Uterine Morcellation 2Health News:As Power Morcellator Controversy Grows, Bernstein Liebhard LLP Comments on Massachusetts Insurers’ Plans to Limit Coverage of Uterine Morcellation 3Health News:As Power Morcellator Controversy Grows, Bernstein Liebhard LLP Comments on Massachusetts Insurers’ Plans to Limit Coverage of Uterine Morcellation 4Health News:Millennium Treatment Group Has a New Website 2Health News:Natural Clear Vision: Review Exposes Kevin Richardson’s Program for Restoring Vision Naturally 2Health News:Beat Eczema: Review Exposes Susan Clark’s Guide to Curing Eczema Naturally 2
... night, Oklahoma Governor Brad Henry vetoed Oklahoma House Bill ... business to conduct research that would kill a human ... risk of death or injury. The Oklahoma House ... Senate failed to follow suit.The bill would have banned ...
... Washington G-2 Reports releases top-10 trends to watch ... Demand for outpatient diagnostic imaging has never ... increase over the next three years. In fact, ... increase by double digits across all modalities (CT, ...
... can help physicians objectively assess a patient,s breast density ... changes that may suggest cancer, according to a study ... of Maryland School of Medicine, Baltimore, MD. ... estimates mammographic breast density. Results obtained by the computer ...
... (VQ) as an alternative to CT for the diagnosis ... patient exposure, according to a study performed at Albert ... NY. "VQ scanning is a nuclear test which shows ... is a pulmonary embolism, the air can enter normally, ...
... breeding techniques, shed light on human genome , , THURSDAY, April ... researchers in 25 countries has succeeded in sequencing the entire ... implications for understanding the biology of this bovine and related ... said the authors of two reports in the April 24 ...
... USA applauds President Obama,s national call for volunteerism and urges ... their local communities. President Obama recently signed a new ... learn about volunteer and service opportunities. "We need your service ... not going to tell you what your role should be. ...
Cached Medicine News:Health News:Demand for Outpatient Diagnostic Imaging Forecast to Increase by Double Digits Across All Modalities Through 2012 2Health News:New computer algorithm helps physicians determine and monitor breast density 2Health News:Scientists From 25 Countries Sequence Cow Genome 2Health News:Scientists From 25 Countries Sequence Cow Genome 3Health News:YWCA USA Applauds President Obama's National Call for Volunteer Service 2
(Date:8/22/2014)... , Aug. 22, 2014 /CNW/ - On Aug. ... , the Canadian Medical Association (CMA) voted on ... any plant material, including medicinal cannabis. Bedrocan Cannabis Corp., ... supportive of the CMA motion. The CMA ... particular hazards associated with smoking any plant material, including ...
(Date:8/22/2014)... Aug. 22, 2014 Research and Markets ... Research Products: Opportunities, Tools & Technologies" report to ... stem cell research products or you wish to expand ... lucrative this market is. The authors know you want ... effectively to scientists, to generate improved revenues and take ...
(Date:8/22/2014)... Mich. , Aug. 22, 2014  Dr. ... Who with the Diamond Lifetime Achievement Award in the ... as CEO of Cardiovascular Clinical Associates. Dr. ... in managing all major aspects of the business; overseeing ... well as primary coronary intervention. In addition, he is ...
Breaking Medicine Technology:Bedrocan Canada Statement Regarding Canadian Medical Association Motion on Smoking Marijuana 2Global Stem Cell Research Products: Opportunities, Tools & Technologies - 2014 Report 2Global Stem Cell Research Products: Opportunities, Tools & Technologies - 2014 Report 3Dr. Mark Rasak, MD is recognized by Continental Who's Who with the Diamond Lifetime Achievement Award 2
... CARLSBAD, Calif., Oct. 18 Excaliard Pharmaceuticals, Inc. ... as Chairman of its Board of Directors. Mr. ... years of experience in successfully developing and commercializing ... field.  Excaliard Pharmaceuticals, Inc. is a biotechnology company ...
... Bend Research Inc. ( www.bendres.com ), a ... announced today it has extended its drug discovery ... Company ( www.bms.com ) (NYSE: BMY ... Under the terms of the 3-year agreement, Bristol-Myers ...
Cached Medicine Technology:Excaliard Pharmaceuticals Appoints Thomas Wiggans to Chairman of the Board 2Bend Research Announces Extension of Drug-Discovery Enablement and Development Services Agreement With Bristol-Myers Squibb 2
... national reference laboratory and a worldwide leader ... offers an extensive test menu of highly ... and anatomic pathology. Owned by the University ... than half of the nation's university teaching ...
... Laboratories is a national reference laboratory and ... and development. ARUP offers an extensive test ... tests in clinical and anatomic pathology. Owned ... clients include more than half of the ...
... is a national reference laboratory and a ... development. ARUP offers an extensive test menu ... in clinical and anatomic pathology. Owned by ... include more than half of the nation's ...
... national reference laboratory and a worldwide leader ... offers an extensive test menu of highly ... and anatomic pathology. Owned by the University ... than half of the nation's university teaching ...
Medicine Products: