Navigation Links
Penn researchers demonstrate efficacy of non-Hodgkin's lymphoma vaccine
Date:10/18/2011

PHILADELPHIA An experimental vaccine developed by researchers at the University of Pennsylvania's schools of Medicine and Veterinary Medicine is the first veterinary cancer vaccine of its kind that shows an increase in survival time for dogs with spontaneous non-Hodgkin's lymphoma. The work shows for the first time the feasibility and therapeutic efficacy of this alternative cell-based vaccine, which could be employed in the treatment of a number of different cancer types.

The research was conducted by Nicola Mason, assistant professor of medicine at Penn Vet; Robert H. Vonderheide, associate professor of hematology and oncology at the Perelman School of Medicine; and Karin U. Sorenmo, associate professor of oncology at Penn Vet. Erika Krick, Beth Overley and Thomas P. Gregor of Penn Vet and Christina M. Coughlin of the School of Medicine also contributed to the research.

Their work was published in the open access journal PLOS ONE.

The team recruited dogs that were brought to Penn's Matthew J. Ryan Veterinary Hospital with newly diagnosed non-Hodgkin's lymphoma to receive the experimental vaccine following standard induction chemotherapy and confirmation of clinical remission. The goal of the study was to determine whether the vaccine would prevent or prolong time to a relapse, a common scenario in both humans and dogs with NHL.

"We vaccinated dogs, which were in clinical remission following chemotherapy, three times," Mason said. "We then tracked them over several years to see if the vaccine would prevent relapse and would prolong overall survival.

"We found that, although the vaccinated dogs still relapsed with clinical disease when they were treated with rescue chemotherapy, they had significantly increased overall survival times when compared to an unvaccinated control group. Some of these dogs are still alive and cancer free more than three years later.

"The results with these dogs indicate that our immunotherapy and rescue chemotherapy appear to act synergistically to prevent a second relapse a phenomenon that has been previously recognized in human patients treated with other types of immunotherapy," she said.

Previous cell based vaccines have utilized genetically engineering dendritic cells which are part of the immune system to stimulate immune responses against cancers. Similar to using weakened viruses in traditional vaccines, scientists load these cells with tumor proteins and inject the cells back into the patient's body. Such cell-based vaccines are already being used to treat prostate cancer in humans, but engineering these cells is expensive and time consuming. Furthermore, patients must also endure long, leukapheresis sessions in which the necessary dendritic cells are harvested from their blood.

The Penn team hypothesized that another kind of immune cell, B-cells, could work just as well under the right conditions. Unlike dendritic cells, many B-cells can be grown from a small blood sample, removing the requirement for leukapheresis.

Mason's team made the vaccine by culturing B-cells from the blood taken from the dogs with NHL. These cells were then loaded with RNA that had been isolated from the patient's own tumor.

The results were impressive.

"Though vaccinated and unvaccinated dogs relapsed with clinical disease at the same time, 40 percent of vaccinated dogs that relapsed experienced long-term survival after a second round of chemotherapy; only 7 percent of unvaccinated dogs that relapsed and were treated with the same rescue chemotherapy protocol survived long term," Mason said. "Furthermore, when the vaccinated long-term survivors did eventually die, they showed no evidence of lymphoma on full necropsy."

While the molecular mechanisms responsible for these observed synergistic effects are currently unknown, Mason believes that the vaccine-primed immune system may be boosted by the effects of rescue chemotherapy leading to long term second remissions.

Though the increases in long-term survival are already unprecedented and the proof-of-concept for B-cell-based cancer vaccines represents a step forward in cell-based vaccine development, future research could have even more exciting results.

"These dogs just received three doses of vaccine, three weeks apart. If we kept boosting the immune system in this way by vaccination, perhaps the dogs would not relapse in the first place" Mason said.

Work is now underway to streamline B-cell vaccine generation and initiate further clinical trials aimed at optimizing this novel cell-based approach.


'/>"/>

Contact: Evan Lerner
elerner@upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert

Related medicine news :

1. Miriam Hospital researchers pilot new acute hepatitis C screening strategy for HIV-infected patients
2. Researchers discover why steroid treatment for COPD is ineffective
3. Researchers find possible link between bacterium and colon cancer
4. Researchers engineer a new way to inhibit allergic reactions without side effects
5. National Jewish Health researchers awarded $13 million to evaluate treatments for toxic gases
6. Researchers discover hidden genetic influence on cancer
7. Toronto researchers find first physical evidence bilingualism delays onset of Alzheimers symptoms
8. Researchers track number of doctors disciplined and why
9. UCLA researchers develop new way to screen for brain cancer stem cell killers
10. Researchers find race disparity in post-hospital arrival homicide deaths at trauma centers
11. Researchers Assess What Works Best to Prevent PTSD
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/16/2017)... ... January 16, 2017 , ... Date aired: January 9, ... , Sharon Kleyne, America’s leading water educator, researcher and advocate welcomed health activist, ... The Sharon Kleyne Hour Power of Water, Global Climate Change and Your Health ...
(Date:1/16/2017)... ... January 16, 2017 , ... In 1985, ... and high-level training standards to an international multidisciplinary group of healthcare treatment providers ... a way to further its mission at the grassroots level, iaedp launched MemberSHARE, ...
(Date:1/16/2017)... ... ... San Francisco dentist, Dr. Ben Amini , the founder at CitiDent, ... capable of taking digital impressions of teeth and gums. Conventional bite impressions have lagged ... , in terms of speed, efficiency and patient comfort. Increasingly, digital impressions are being ...
(Date:1/16/2017)... ... January 16, 2017 , ... NexTec Group has been selected ... 100 organizations honored for their accomplishments in the field of midmarket financial software. , ... and recognition, and innovation. Selection is not based on revenue and those firms chosen ...
(Date:1/15/2017)... ... January 15, 2017 , ... Going above and ... that strives to better communities around the world by offering the Gensuite team ... the opportunity for team members to become involved in a cause that is ...
Breaking Medicine News(10 mins):
(Date:1/17/2017)... Jan. 17, 2017 Tempus, a technology ... and University Hospitals Seidman Cancer Center have joined ... genomic data, advanced molecular analysis and clinical decision ... and healthcare providers. As part ... and transcriptomic sequencing and analysis for ovarian and ...
(Date:1/17/2017)... Md. , Jan. 17, 2017  CSSi, the ... for the clinical research industry, will exhibit at the ... Operations Executives (SCOPE 2017), taking place from Tuesday, January ... Regency Miami in Miami, FL. ... newest proprietary technology platform, SmartStudy™ EMR, which provides a ...
(Date:1/17/2017)... , Jan. 17, 2017  Bayer today announced the ... its Pharmaceuticals business in the Americas, comprising the ... and Latin America . In this role, ... of the Board of Management of Bayer AG and President ... and highly respected leader in the pharmaceutical industry and I,m ...
Breaking Medicine Technology: