Navigation Links
Penn animal study identifies new DNA weapon against avian flu
Date:7/1/2008

PHILADELPHIA - Researchers at the University of Pennsylvania School of Medicine have identified a potential new way to vaccinate against avian flu. By delivering vaccine via DNA constructed to build antigens against flu, along with a minute electric pulse, researchers have immunized experimental animals against various strains of the virus. This approach could allow for the build up of vaccine reserves that could be easily and effectively dispensed in case of an epidemic. This study was published last week in PLoS ONE.

"This is the first study to show that a single DNA vaccine can induce protection against strains of pandemic flu in many animal models, including primates," says David B. Weiner, Ph.D., Professor of Pathology and Laboratory Medicine. "With this type of vaccine, we can generate a single construct of a pandemic flu vaccine that will give much broader protection."

Traditional vaccines expose a formulation of a specific strain of flu to the body so it can create immune responses against that specific strain. Conversely, a DNA vaccine becomes part of the cell, giving it the blueprint it needs to build antigens that can induce responses that target diverse strains of pandemic flu.

Avian flu is tricky. Not only is it deadly, but it mutates quickly, generating different strains that escape an immune response targeted against one single strain. Preparing effective vaccines for pandemic flu in advance with either live or killed viruses, which protect against only one or few cross-strains, is therefore very difficult. How to predict which strain of avian flu may appear at any time is difficult. "We are always behind in creating a vaccine that can effectively protect against that specific strain," notes Weiner.

Instead of injecting a live or killed virus, Penn researchers injected three different species of animal models with synthetic DNA vaccines that are not taken from the flu microbe, but trick the immune system into mounting a broad response against pandemic flu, including strains to which the immune system was never exposed. Antibodies induced by the vaccine rapidly reached protective levels in all three animal species.

"The synthetic DNA vaccines designed in this study customize the antigen to induce more broad immune responses against the pathogen," says Weiner.

Researchers found evidence of two types of immune responses T lymphocytes and antibodies -- in all three types of animal models. Two types of animal models (mice and ferrets) were protected from both disease and mortality when exposed to avian flu.

To ensure increased DNA delivery, the researchers administered the vaccine in combination with electroporation, a small, harmless electric charge that opens up cell pores facilitating increased entry of the DNA vaccine into cells.

If proven in humans, this research could lead the way to preparing against an outbreak of avian flu. Because these synthetic DNA vaccines are effective against multiple cross strains, vaccines could be created, stockpiled, prior to a pandemic, and thus be delivered quickly in the event of an outbreak, surmise the researchers.

This study has shown other advantages of DNA vaccines. On one hand, killed vaccines, which involve the injection of a dead portion of a virus, are relatively safe but usually effective at producing only a strong cellular immunity. Live vaccines, which involve the injection of a form of a live virus, can have increased manufacturing and some safety issues. Both of these vaccine strategies may have concerns in persons with certain allergies (egg for example) as current manufacturing methods rely on egg based production technologies. On the other hand, DNA vaccines preclude the need to create live tissue samples, which presents risk to those working with the virus.

"DNA vaccines have the benefits and avoid many conceptual negatives of other types of traditional vaccines," says Weiner.

This research also has implications for non-avian types of flu. Every year, scientists try to guess what strain of the year will be that creates the common flu. Sometimes their educated guess is wrong, which is why last year's influenza vaccine worked only 30 percent of the time. Designing traditional vaccines in combination with the DNA platform may be a partial solution to this dilemma, predicts Weiner.


'/>"/>

Contact: Karen Kreeger
karen.kreeger@uphs.upenn.edu
215-349-5658
University of Pennsylvania School of Medicine
Source:Eurekalert

Related medicine news :

1. Newly approved ocular safety methods reduce animal testing
2. Data Published in Plastic and Reconstructive Surgery Show Cohera Medicals Surgical Adhesive, TissuGlu(R), Prevents Seroma Formation in Animal Abdominoplasty Model
3. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow in Indianapolis is Participating
4. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow in Cincinnati is Participating
5. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow Locations in Texas Participating
6. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow Locations in New Jersey Participating
7. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow Locations in Minnesota Participating
8. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow Locations in Michigan Participating
9. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow Locations in Colorado Participating
10. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas; Camp Bow Wow Locations in California Participating
11. Camp Bow Wow(R) Launches Fundraising Effort to Help Cure Cancer in Dogs, via Morris Animal Foundations Canine Cancer Campaign - Dogs Get Bandanas
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/25/2017)... Marietta, GA (PRWEB) , ... April 25, 2017 ... ... implant and instrument solutions, announces the continuation of its strategic partnership with and ... surgeons in developing nations in the safe and effective management of complex spine ...
(Date:4/24/2017)... (PRWEB) , ... April 24, 2017 , ... The Santana ... during ATA 2017: Telehealth 2.0 — the American Telemedicine Association’s annual conference, on April ... Telehealth Project for using telemedicine to improve the lives of the poor and underserved ...
(Date:4/24/2017)... ... ... The bar for just about everything—apparel, eyewear, cars and even phone cases—is ... and film stardom.(1) Not to be left out is that most desired of commodities, ... gamut from being encrusted with jewels and precious metals to, at the very least, ...
(Date:4/24/2017)... ... 24, 2017 , ... Veteran Theresa James awoke to the sound of her ... In “Healing Tears,” James depicts every parent’s worst nightmare, when her three children were ... conversations and situations throughout my divorce,” James said. “After the death of my children, ...
(Date:4/24/2017)... ... April 24, 2017 , ... My T Chai, a South African ... now available for purchase on RevNutrition.com, a popular website specializing in sales of nutritional ... popularized in ancient India and Siam. It spread across Asia and Africa quickly, and ...
Breaking Medicine News(10 mins):
(Date:4/20/2017)... Research and Markets has announced the addition of ... By Service (Manufacturing, Research), By Country, (Brazil, Mexico, Colombia, Argentina, ... to their offering. ... The Latin American pharmaceutical contract manufacturing services market is anticipated ... drug registration cost in Latin American countries and continuous economic ...
(Date:4/19/2017)... -- Global Surgical Drainage Device Market: Overview ... excess liquid and air. The fluid to be drained ... Surgical drains are used in a wide variety of ... surgery, neurosurgery, plastic surgery etc. Common use of surgical ... fluid e.g. blood or pus. Surgical drains are available ...
(Date:4/19/2017)... LONDON , April 19, 2017 ... to stimulate an immune response in pets such ... vaccine products are of various types such as ... Vaccines, Toxoid Vaccines, DNA Vaccines and Recombinant Vaccines. ... such as virus or bacteria, which have been ...
Breaking Medicine Technology: