Navigation Links
PET scanning probes reveal different cell function within the immune system
Date:5/18/2010

A commonly used probe for Positron Emission Tomography (PET) scanning and a new probe developed by researchers at UCLA reveal different functions in diverse cells of the immune system, providing a non-invasive and much clearer picture of an immune response in action.

The probes, the commonly used FDG that measures cellular glucose metabolism, and FAC, developed at UCLA and which measures the activity of a distinct biochemical pathway, work better when used in combination than either does alone. In addition to revealing the extent and cellular composition of an immune response, the probes also may be useful in evaluating therapies that target different cellular components of the immune system, said Dr. Owen Witte, a professor of microbiology, immunology and molecular genetics, a Howard Hughes Medical Institute investigator and senior author of the study.

"We demonstrated with this study that each probe targets different cells in the immune system with a high degree of specificity," said Witte, director of the UCLA Broad Stem Cell Research Center and a Jonsson Cancer Center researcher. "When cells are activated to do their job as an immune cell, the FDG probe is good at recognizing the subset of activated macrophages, while the FAC probe is good at recognizing the activated lymphocytes, as well as the macrophages. When tested sequentially, the combined information from the scans using the two probes gives you a better status of immune response."

The study, with lead author Evan Nair-Gill, a student in the campus' Medical Scientist Training Program, was conducted on mice bearing virally-induced sarcomas. The article appears today in the early online edition of the Journal of Clinical Investigation. Testing the probes in humans is the next step.

The scans provide clues to how the immune system works, for example, in response to cancer or auto-immune diseases such as rheumatoid arthritis, inflammatory bowel disease and multiple sclerosis, Witte said. They also could be used to see how therapies, such as vaccines and monoclonal antibodies meant to stimulate an immune response, are functioning within the body of a patient.

"This could give us another way to measure the efficacy of certain drugs," Witte said. "With some drugs, you could measure a change in the immune response within a week."

If the drugs are working, Witte said, doctors could stay the course. If they're not working or not working well enough, the therapy could be discontinued, sparing the patient a months-long exposure to an ineffective drug.

The next step will be testing the two probes in humans with a range of diseases, including cancer and auto-immune disorders, to confirm the work.

Witte and his colleagues licensed the FAC probe to Sofie Biosciences, which is owned in part by Witte and other UCLA faculty members. Researchers created the small molecule by slightly altering the molecular structure of one of the most commonly used chemotherapy drugs, gemcitabine. They then added a radiolabel so the cells that take in the probe can be seen during PET scanning.

The probe measures the activity of a fundamental cell biochemical pathway called the DNA salvage pathway, which acts as a recycling mechanism that helps with DNA replication and repair. All cells use this biochemical pathway to different degrees. But in lymphocytes and macrophages that are proliferating during an immune response, the pathway is activated to very high levels. Because of that, the probe accumulates at high levels in those cells, Witte said.


'/>"/>

Contact: Kim Irwin
kirwin@mednet.ucla.edu
310-206-2805
University of California - Los Angeles
Source:Eurekalert

Related medicine news :

1. Symposium focuses on patient safety in CT scanning
2. New form of endoscopic scanning improves detection of precancerous condition
3. Scanning for skin cancer: Infrared system looks for deadly melanoma
4. New e-Forms Management Tools from Access Bridge the Gap Between MEDITECH Magic and Scanning and Archiving for Hospitals
5. SecureWorks Extends PCI Approved Scanning Vendor Solutions
6. Lab that probes genetic clues to disease is poised for major expansion
7. Fluorescent probes light up cancerous tumors
8. Epione Reveals a ‘Bright' New Solution for Dull, Sun Damaged Skin
9. Independent Study Reveals Family Bonds made Stronger on Vacations where Animals are Involved
10. Statistics Reveal Women Choose Engagement Rings Before Men
11. Mayo Clinic study reveals neighborhood asthma risks
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/26/2017)... ... July 26, 2017 , ... The Amnesty Rally 2017 will be ... Park at 3313 Bernstein Park Drive in Monroe (adjacent to the Louisiana Purchase Gardens ... help reduce gun violence throughout Monroe. Citizens can turn in unregistered, illegally-held and imitation ...
(Date:7/26/2017)... ... 26, 2017 , ... BioPlus Specialty Pharmacy (BioPlus), ... of the HCV Treatment Path app (version 4.4.0) to keep health care providers ... All of the latest HCV medications are included in this app, including Vosevi™ ...
(Date:7/25/2017)... (PRWEB) , ... July 25, 2017 , ... “Now Hiring!” ... St. Louis, Missouri. The company has achieved record-breaking sales for over 30 consecutive ... the next 12 months. , The upcoming year offers excitement and growth at HDIS, ...
(Date:7/25/2017)... Florida (PRWEB) , ... July 25, 2017 , ... ... Jennifer Haller, to the sales department joining as Regional Account Manager for the ... with contracted customers in the liquidation of their obsolete medical assets. , Jennifer ...
(Date:7/25/2017)... ... July 25, 2017 , ... SureVest Insurance Group, a locally ... across North Carolina, is launching a charity event in conjunction with the Triangle ... ongoing fight against bladder cancer. , The Bladder Cancer Advocacy Network (BCAN) operates ...
Breaking Medicine News(10 mins):
(Date:7/12/2017)...  Eli Lilly and Company (NYSE: LLY ) ... resolve pending patent litigation in the U.S. District Court for ... the Cialis ® (tadalafil) unit dose patent. This patent ... part of the agreement, Cialis exclusivity is now expected to ... "The unit dose patent for Cialis is valid and infringed ...
(Date:7/11/2017)... , July 11, 2017  Sysmex America, Inc., ... diagnostic testing equipment as well as middleware information ... way to make quality assurance easier and more ... is well known for the innovation that it ... Monitor elevates quality assurance processes to a new ...
(Date:7/10/2017)... , July 10, 2017 The Institute for ... methods, is the recipient of a VITROCELL® inhalation exposure ... International Science Consortium. The device, which is designed to ... expose human lung cells to airborne test materials in ... use the VITROCELL® system for testing combustible tobacco products, ...
Breaking Medicine Technology: