Navigation Links
Novel technique reveals both gene number and protein expression simultaneously

COLUMBUS, Ohio -- Researchers have discovered a method for simultaneously visualizing gene number and protein expression in individual cells. The fluorescence microscopy technique could permit a detailed analysis of the relationship between gene status and expression of the corresponding protein in cells and tissues, and bring a clearer understanding of cancer and other complex diseases, according to researchers who led the study.

The new technique is called the fluorescent in situ gene protein assay. It combines traditional fluorescent in situ hybridization (FISH) with the in situ proximity ligation assay, which is capable of resolving individual protein molecules.

"To my knowledge, this is the first technique that allows us to concurrently address gene activity and corresponding protein expression in the same cells," says co-principal investigator Dr. Arnab Chakravarti, chair and professor of radiation oncology and co-director of the Brain Tumor Program at the Ohio State University Comprehensive Cancer Center Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC - James).

"The ability to resolve gene and protein-expression changes across a tumor could help us understand what drives tumor behavior overall," Chakravarti says

The new assay is described in the August issue of the journal Neuro-Oncology. For this study, principal investigator Dr. Markus Bredel, an associate professor at the University of Alabama Birmingham, and an adjunct associate professor radiation oncology at the OSUCCC James, along with Chakravarti and their collaborators first assayed fixed human glioblastoma tumor cells, then paraffin-embedded human glioblastoma tissue. In both cases, the researchers assayed for overexpression of a mutant form of the epidermal growth factor receptor gene, EGFRvIII, and for levels of its truncated protein in glioblastoma.

"This method has potential to perform a detailed analysis of the relationship between cancer gene status and corresponding protein expression in cells and tissues," Bredel says. "We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving cancer gene and protein patterns simultaneously on a cell-by-cell basis, which is particularly important in heterogeneous diseases such cancers."

The implications of the assay include the following:

  • It is particular relevant to cancer research due to the role of epigenetic and posttranscriptional regulation.
  • The ability to correlate gene and protein information in the same cells might increase the reliability of biomarker screens.
  • It might aid in therapeutic decision making when screening for only the gene or the protein yields indeterminate results.
  • It can be applied to gene-transfection studies that use 'knock-in' models to study the effects of gene number on protein expression.

"We believe this assay is broadly applicable to disease-oriented and cell- and molecular-biology research," Chakravarti says.

Contact: Darrell E. Ward
Ohio State University Medical Center

Related medicine news :

1. NIH grants to Childrens Hospital will advance novel stem cell treatments for blood disorders
2. A novel in vitro model for light-induced wound healing
3. Novel program translates behavioral and social science research into treatments to reduce obesity
4. Reovirus may be a novel approach to prostate cancer treatment
5. Novel stroke treatment passes safety stage of UCI-led clinical trial
6. Novel medical home program for pediatric patients, families cuts ER visits in half
7. MessageSolution First in the Market to Offer All-in-One, Integrated Cloud-Based Archiving for Email, File Systems and SharePoint at Novell BrainShare 2010
8. Novel Parkinson's Treatment Strategy Involves Cell Transplantation
9. Novel Method Eyed for Normalizing Blood Sugar
10. Novel soy germ-based dietary supplement, SE5-OH containing natural S-Equol, examined for safety and influence on hormones in pre- and post-menopausal women
11. Novel nanoparticles prevent radiation damage
Post Your Comments:
(Date:10/13/2017)... (PRWEB) , ... October 13, 2017 , ... ... agency serving communities in the greater Dallas, Miami, and Raleigh regions, is organizing ... fighting to overcome a rare and deadly chromosome abnormality. , After struggling since ...
(Date:10/13/2017)... ... October 13, 2017 , ... The ... to promote standards of excellence for the field of eating disorders, announces the ... – 25, 2018 in Orlando, Florida at the Omni Resort at ChampionsGate. ...
(Date:10/13/2017)... ... October 13, 2017 , ... Many families have long-term insurance that covers ... companies have a waiver for care if the client has a cognitive impairment diagnosis. ... pays for care, is often waived, so the benefits from their insurance start immediately,” ...
(Date:10/13/2017)... ... October 13, 2017 , ... Talented ... the lowdown on sciatica in a new episode of "Success Files," which is ... events and innovation and investigates each subject in-depth with passion and integrity. , ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... sleep apnea using cutting-edge Oventus O2Vent technology. As many as 18 ... characterized by frequent cessation in breathing. Oral appliances can offer significant relief to ...
Breaking Medicine News(10 mins):
(Date:10/4/2017)...  According to the Centers for Disease Control and Prevention (CDC), ... Urgent Care is helping communities across Massachusetts , ... offering no-cost* flu shots through the end of the month. ... regulations. ... a flu shot is by the end of October, according to the ...
(Date:10/2/2017)... 2017 Halo Labs announces the European launch of their ... HORIZON at MIBio 2017 in Cambridge, U.K ... matter in biopharmaceutical samples with unprecedented speed and sensitivity while using ... Backgrounded Membrane Imaging. ... subvisible particle analysis system ...
(Date:9/27/2017)... and NEW YORK , Sept. 27, 2017 /PRNewswire/ ... health and big data solutions, today announced that its MyDario product is ... your local TV listings for when The Dr. Oz Show airs in ... The nine-time ... month. ...
Breaking Medicine Technology: