Navigation Links
New vaccine approach prevents/reverses diabetes in lab study at Children's Hospital of Pittsburgh
Date:5/28/2008

Microspheres carrying targeted nucleic acid molecules fabricated in the laboratory have been shown to prevent and even reverse new-onset cases of type 1 diabetes in animal models. The results of these studies were reported by diabetes researchers at the John G. Rangos Sr. Research Center at Childrens Hospital of Pittsburgh of UPMC and Baxter Healthcare Corporation.

In a research study at Childrens Hospital, the scientists injected the microspheres under the skin near the pancreas of mice with autoimmune diabetes. The microspheres were then captured by white blood cells known as dendritic cells which released the nucleic acid molecules within the dendritic cells. The released molecules reprogrammed these cells, and then migrated to the pancreas. There, they turned off the immune system attack on insulin-producing beta cells. Within weeks, the diabetic mice were producing insulin again with reduced blood glucose levels.

Results of the microsphere study are published in the June issue of Diabetes, the journal of the American Diabetes Association.

In type 1 diabetes, T cells from the immune system travel to the pancreas and destroy beta cells, which produce insulin. The scientists led by Massimo Trucco, MD, and Nick Giannoukakis, PhD found that the microspheres reprogram dendritic cells to block the signaling mechanism that sends T cells to destroy beta cells. The microsphere research builds on previous research by Drs. Giannoukakis and Trucco in which they used dendritic cells delivered to the pancreas in another method to turn off the immune systems attack on insulin-producing beta cells, thereby allowing the cells of the pancreas to recover and begin producing insulin again.

Drs. Trucco and Giannoukakis anticipate that the latest research involving microspheres represents a significant improvement over their previous approach to extract (through a process known as leukapheresis) and reprogram the dendritic cells.

The microspheres prevented the onset of type 1 diabetes and, most importantly, exhibited a capacity to reverse hyperglycemia, suggesting a potential to reverse type 1 diabetes in new-onset patients, said Dr. Trucco, chief of the Division of Immunogenetics at Childrens. This novel microsphere approach represents for the first time a vaccine with the potential to suppress and reverse diabetes. This finding holds true promise for clinical testing in people with type 1 diabetes.

Currently, Drs. Trucco and Giannoukakis are conducting a clinical trial of their leukapheresis-based dendritic cell approach in humans at Childrens. This Phase 1 clinical trial has been approved by the U.S. Food and Drug Administration (FDA).

Our ultimate goal is to offer this dendritic cell vaccine or microsphere-based therapy to children at risk for or newly diagnosed with type 1 diabetes. We want to make the procedure as safe and comfortable as possible, Dr. Giannoukakis said.

The trial began late last year and enrollment is ongoing. The study, which plans to enroll a total of 15 adults over age 18 with type 1 diabetes, is expected to conclude later this year.

If the leukapheresis-based approach continues to show exceptional safety, the researchers hope to launch a national clinical trial that will assess the effectiveness of the dendritic cells in pediatric patients to prevent diabetes or reverse the disease right after it is clinically confirmed. At a later date, it is anticipated that Baxter Healthcare will collaborate with Drs. Trucco and Giannoukakis in a clinical trial utilizing the unique microsphere-based approach.

Leukapheresis is a process that allows for the collection of dendritic cell precursors from the patients in the study, which takes two to four hours. After the precursors are collected, they are treated in the lab with specific growth factors that turn them into dendritic cells. The growth factors are also combined with short DNA sequences that specifically block the expression of molecules that are found at the surface of dendritic cells known as CD40, CD80 and CD86. Once these reprogrammed dendritic cells are tested in the lab, they are injected back into the patient. They then orchestrate an anti-diabetic effect by suppressing the activity of T-cells which are responsible for the impairment and destruction of the pancreatic insulin-producing cells.

Using microspheres will be much less invasive for the patient and much more efficient for clinicians. We wouldnt need to harvest a patients dendritic cells, and it would eliminate the need to genetically reprogram the dendritic cells in a sterile, off-site facility. Instead, the patient would receive the microsphere injection with a small needle in a clinic setting in a matter of minutes, Dr. Giannoukakis said.


'/>"/>

Contact: Marc Lukasiak
marc.lukasiak@chp.edu
412-692-7919
Children's Hospital of Pittsburgh
Source:Eurekalert

Related medicine news :

1. Jenny McCarthy & Jim Carrey Host the Historic Green Our Vaccines March and Rally in Washington DC on June 4, 2008
2. Study finds unique HIV vaccine formula elicits strong immune responses
3. AVANT Immunotherapeutics Announces Hart-Scott Rodino Clearance to License Cancer Vaccine to Pfizer
4. NIAID to advance B-cell approach to HIV vaccines
5. Vaccine triggers immune response, prevents Alzheimers
6. Prostate Cancer Vaccine Looks Promising in Early Trial
7. AIDS Action Urges Everyone to Help End the AIDS Epidemic by Being Part of the Search for an HIV Vaccine
8. World AIDS Vaccine Day 2008
9. Chemotherapy might help cancer vaccines work
10. CDC Recommends Shingles Vaccine for Those Over 60
11. Dutch Authorize Manufacture of Vaccinogens New Cancer Vaccine; License Clears Path to European Production, Final FDA Trial
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/29/2016)... (PRWEB) , ... April 29, 2016 , ... Regenerative ... Best Places to Work employee satisfaction survey, earning them second place for Tampa’s Best ... with 25-49 employees. , “This is a great accomplishment for our team,” says ...
(Date:4/29/2016)... Village, CA (PRWEB) , ... April 29, 2016 ... ... Philadelphia Pet Expo to promote their animal line of probiotics, Petbiotics ™, ... see the large turnout of animal rescue groups networking for their non-profit organizations. ...
(Date:4/29/2016)... ... April 29, 2016 , ... ... detailing the company’s use of Microsoft Dynamics GP for one their long-term care ... solution that integrated Dynamics GP with key clinical management systems, and delivered a ...
(Date:4/29/2016)... ... 2016 , ... Yisrayl Hawkins, Pastor and Overseer at The House of Yahweh ... AD, saying it is time to set the record straight. Traditionally, BC and AD ... cannot possibly be true and offers explanation. , “To start with, the Name of ...
(Date:4/28/2016)... , ... April 29, 2016 , ... Monthly subscription boxes ... on the market. They recently reviewed the ZEN BOX , the aromatherapy subscription ... two single essential oils (like lavender or frankincense) and one Zen Blend (like immune ...
Breaking Medicine News(10 mins):
(Date:4/27/2016)... , April 27, 2016 ... initiative to save newborns   ... for women & newborns in collaboration with Breast Milk Foundation ... the first Pasteurized Human Milk Bank, ,Amaara, in Delhi-NCR today. ... nutritional food source for infants and should be available to ...
(Date:4/27/2016)... NEW YORK , April 27, 2016 /PRNewswire/ ... OASM ), a developer of a new ... today announced positive overall survival results for Paclical/Apealea ... total of 789 patients with epithelial ovarian cancer. ... treatment groups: Paclical/Apealea in combination with carboplatin versus ...
(Date:4/26/2016)... Mich. , April 26, 2016 Diplomat ... promotion of Jennifer Hagerman , Pharm D., to ... her growing role at Diplomat, Hagerman will continue to ... the company that delivers custom education and training to ... the specialty pharmacy industry. Diplomat University also houses the ...
Breaking Medicine Technology: