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New tool for cell research may help unravel secrets of disease
Date:1/13/2011

e in vivo, or live cell, environment.

To do this, they rely upon the use of gold nanorods, which are only 25 by 73 nanometers in size (a well-packed bundle of 1000 nanorods has the same diameter as a human hair). In live cells, these non-toxic nanorods scatter light differently depending upon their orientation. Using a technique called differential interference contrast microscopy, or DIC, Fang's team can capture both the orientation and the position of the gold nanorods in addition to the optical image of the cell and, thus, reveal a particle's 5D (3 spatial coordinates and 2 orientation angles) movement within living cells.

"DIC imagining of this gold nanorod helps give us high angular resolution," says Fang.

"This new technique opens doors to understanding the working mechanism of living nanomachines by revealing their complex internal motions," said Fang. "Studying rotational motions at the nanometer scale inside a living cell is just something that has never been done before." He added that understanding this rotational motion is important in the fight against diseases, such as Alzheimer's, because it can help scientists better understand how neurons are impacted by the environment.


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Contact: Steve Karsjen
karsjen@ameslab.gov
515-294-5643
DOE/Ames Laboratory
Source:Eurekalert  

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