Navigation Links
New testing method hints at garlic's cancer-fighting potential
Date:3/1/2010

COLUMBUS, Ohio Researchers have designed a urine test that can simultaneously measure the extent of a potential carcinogenic process and a marker of garlic consumption in humans.

In a small pilot study, the test suggested that the more garlic people consumed, the lower the levels of the potential carcinogenic process were.

The research is all about body processes associated with nitrogen-containing compounds, scientists say. These processes include nitrosation, or the conversion of some substances found in foods or contaminated water into carcinogens.

"What we were after was developing a method where we could measure in urine two different compounds, one related to the risk for cancer, and the other, which indicates the extent of consumption of garlic," said Earl Harrison, Dean's Distinguished Professor of Human Nutrition at Ohio State, an investigator in Ohio State University's Comprehensive Cancer Center, and senior author of the study.

"Our results showed that those were inversely related to one another meaning that the more we had the marker for garlic consumption, the less there was of the marker for the risk of cancer."

Ultimately, the scientists hope to find that a nutritional intervention could be a way to stop the process that develops these carcinogens. This process is most commonly initiated by exposure to substances called nitrates from certain processed meats or high-heat food preparation practices, or to water contaminated by industry or agricultural runoff.

About 20 percent of nitrates that are consumed convert to nitrites. A cascade of events can convert these compounds into what are called nitrosamines, and many, but not all, nitrosamines are linked to cancer.

Vegetables also contain nitrates, but previous research has suggested that the vitamin C in vegetables lowers the risk that those nitrates will convert to something toxic. Researchers suspected that nutrients in garlic could have similar antioxidant effects as vitamin C.

The study is published in a recent issue of the journal Analytical Biochemistry.

The research began with the small human study based at Penn State University. Researchers there fed participants a weeklong diet lacking any nitrates or garlic. They then gave the participants a dose of sodium nitrate in a formulation that would not become toxic, but which would show a marker in the urine of the potentially toxic process.

Groups were then treated with capsules containing varying levels of garlic: 1, 3 or 5 grams of fresh garlic, or 3 grams of an aged garlic extract. A separate group received 500 milligrams of ascorbic acid, or vitamin C. Both the nitrate formula and treatments were given for seven days. Urine samples were collected from all of the participants every other day for seven days.

That research team then turned to Harrison and colleagues, who explored the methods required to precisely quantify biomarkers in urine for both the garlic consumption and the presence of nitrosoproline, the indicator that nitrosation has occurred.

Harrison's group developed the urine test using a method called gas chromatography-mass spectrometry.

Gas chromatography separates components of a mixture to detect specific substances, and has been used previously to quantify nitrosoproline. The addition of mass spectrometry to the analysis allowed for determination of the chemical structures of molecules in the sample in this case, the presence of a specific compound that is released in urine after garlic is eaten.

When the test was used on the urine samples from the pilot garlic study, it showed that the participants who had taken garlic had lower concentrations of the marker for nitrosation than did those who took no garlic. Though the differences were slight, the consumption of 5 grams of garlic per day was associated with the lowest level of the marker for potential carcinogens. A single garlic clove typically can weigh between 1 and 5 grams.

Vitamin C had a similar effect in lowering the marker for nitrosation.

Harrison, also an investigator in the Center for Advanced Functional Foods Research and Entrepreneurship at the Ohio Agricultural Research and Development Center, noted that previous research has suggested that garlic and other plants with sulfur-containing compounds offer a variety of potential health benefits. Many questions remain about exactly what those benefits are and precisely how garlic works as a nutritional intervention.

"The precise mechanism by which garlic and other compounds affect nitrosation is under extensive investigation, but is not clear at this time," he said.

"What this research does suggest, however, is that garlic may play some role in inhibiting formation of these nitrogen-based toxic substances. This was very small pilot study, so it's also possible that the more garlic you have, the better it would be.

"So if you like garlic and you like garlic-containing foods, go out and have as much as you want. There's no indication it's going to hurt you, and it may well help you."


'/>"/>

Contact: Earl Harrison
harrison.304@osu.edu
614-292-8189
Ohio State University
Source:Eurekalert

Related medicine news :

1. Study says COPD testing is not measuring up
2. Spirometry Testing for COPD Underused
3. Medical Services International Inc. Completes Testing for European Union (EU) Application
4. Medical Services International Received Its First Order for Testing By Regulatory Agencies in the United States
5. Lab-on-chip testing for bird flu developed by Singapore scientists
6. Physicians Wellness Network Announces Lab Testing Services for Consumers built on Microsoft HealthVault
7. PSA Testing Still Valuable for Prostate Cancer
8. Schwarzenegger Signs Bill to Remove Written Informed Consent for HIV Testing; "Most Important Change in Public HIV/AIDS Policy in Years," Says AHF
9. Latinos/Hispanics Across the Nation Recognize National Latino AIDS Awareness Day and Call to Action Leaders in Promoting HIV Testing, Prevention and Access to Care
10. Random drug testing not reliable in keeping teen athletes from using
11. Hepatitis C Testing Recommended for Anyone with a Tattoo
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/26/2017)... ... , ... Amir Qureshi, MD is the first physician in Arkansas to implant ... The Nuvectra™ Algovita SCS System has been FDA approved as a treatment option for ... to introduce the most powerful SCS system and the only stretchable lead on the ...
(Date:5/26/2017)... ... May 26, 2017 , ... A new analysis of community health data ... are located in the Midwest. With the average cost of healthcare rising and the ... with both the quality and affordability of where they live. An annual 2017 report ...
(Date:5/26/2017)... ... May 26, 2017 , ... via seating is proud to ... task chair specifically designed for clinical areas. Genie Copper Mesh is a crossover ... Cupron® to provide customers with a game changing chair that is affordably priced,” ...
(Date:5/26/2017)... ... ... After raising nearly $30,000 on Kickstarter , about three-times its original campaign ... crowdfunding price on Indiegogo . , “Along with creating an anti-stress gadget to ... fidget toy to the market that was made of superior quality and wouldn’t break ...
(Date:5/26/2017)... (PRWEB) , ... May 26, 2017 , ... Silver Birch ... community, which is located on more than four acres of land at 5620 Sohl ... , The 103,000 square-foot building includes 125 studio and one-bedroom apartments. Each of ...
Breaking Medicine News(10 mins):
(Date:5/6/2017)... , May 5, 2017   Provista , a proven ... than 200,000 customers, today announced Jim Cunniff as ... of executive and business experience to Provista, including most recently ... in California . He assumed his new ... is a great fit for Provista," says Jody Hatcher ...
(Date:5/4/2017)... Tenn. , May 4, 2017  A ... Infection Control, Ultraviolet-C light as a ... Tru-D SmartUVC,s ability to reduce bioburden on anesthesia ... bioburden reduction on high-touch, complex medical equipment surfaces ... surgical infections. "This study further validates ...
(Date:5/4/2017)... May 4, 2017  A new tight-tolerance microextrusion ... other highly-engineered materials, is being launched by Natvar, ... been developed in recent years to service a ... surgical applications. More expensive materials such as glass ... tubing due to their ability to consistently hold ...
Breaking Medicine Technology: