Navigation Links
New test for safer biomedical research results
Date:7/30/2009

In cancer research, as in most other biomedical sciences, they are playing a key role: living cells, kept in sterile plastic containers with red culture media populating incubators in laboratories around the world. But do researchers always know what is really living in their culture dishes? Under the microscope, different cell lines are almost impossible to distinguish from each other. When these important research objects stop growing without apparent reason is it because of the manipulations by the scientists or because of an invisible viral or bacterial infection?

Contaminations with other cell lines or pathogenic agents are a common and well-known problem. Often they are the reason why cell experiments fail to produce useable or reproducible results. Even worse, laboratory staff can get infected with dangerous pathogens from a cell culture.

To make those important cell culture experiments safer, DKFZ researchers Dr. Markus Schmitt and Dr. Michael Pawlita have developed a test which is able to identify 37 different cell contaminations in a single run. The researchers have tested the system in over 700 samples from different research labs and have now published their results.

The method called "Multiplex cell Contamination Test" (McCT) detects not only wide-spread viruses but also a number of mycoplasmas, which are considered the major contaminators of cell cultures. In addition, the test checks the cells for their origin. Thus, if dog genetic material is found in what are supposed to be monkey cells, then a contamination of the cell culture is obvious. The test also includes detection of commonly used standard cell lines. Contamination with the fast-growing cancer cell line HeLa, for example, is a dreaded source of false results.

Pawlita and Schmitt found contaminations in a high percentage of cell samples. Twenty-two percent of tested cultures were contaminated with one of the various types of the parasitic bacterium called mycoplasma. "What we noticed about the results," says Markus Schmitt, "was that contaminations were frequent in some laboratories, while others sent in cultures that were constantly clean. Thus, care in laboratory work seems to play an important role."

The test is highly specific and needs no more than ten copies of foreign DNA in the cell sample to be positive. This is a sensitivity which is comparable to or even higher than those of previously available commercial mycoplasma tests. McCT results are reproducible to 99.6 percent. The method is based on multiplication of specific DNA sequences by polymerase chain reaction and subsequent detection of the multiplied DNA regions. A special advantage of the new test is that it can be carried out on a high-throughput basis. The DKFZ researchers can manage up to 1,000 tests per week.

Schmitt und Pawlita offer the service to external scientists and research institutes via the Steinbeis Transfer Center "Multiplexion", a DKFZ spin-off. If you are interested, please visit www.multiplexion.com for more information about the conditions.


'/>"/>

Contact: Dr. Sibylle Kohlstdt
s.kohlstaedt@dkfz.de
Helmholtz Association of German Research Centres
Source:Eurekalert

Related medicine news :

1. Study Finds Weight-Loss Surgery Safer Than Thought
2. Smokeless tobacco safer than smoking
3. Seven Tips for Safer Home Swimming Pools and Hot Tubs
4. New Anesthesia May Be Safer for Critically Ill
5. Mass. General team develops potentially safer general anesthetic
6. Making the difficult task of transporting a child with special needs safer
7. Prostate Drug Appears Safer Than Thought
8. Consumers are Looking to Boiron Arnicare(R) as a Safer Option as FDA Enforces Stricter Labeling for Many Pain Relievers
9. Mussel-Based Glue May Make Surgery Safer
10. Oh Baby, Interventional Radiology Makes Childbirth Safer
11. New information points to safer methadone use for treatment of pain and addiction
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/24/2017)... ... 24, 2017 , ... Yisrayl Hawkins, Pastor and Overseer at The House of ... generation. Yisrayl makes an astounding statement when he says that the entire Bible ... Bible details the current times so plainly that anyone should be able to see ...
(Date:2/24/2017)... ... February 24, 2017 , ... Rosica Communications, a national ... , media relations, social media, content marketing and SEO, is now offering direct, ... an intuitive marketing automation platform. , Rosica will now offer the platform to ...
(Date:2/23/2017)... ... February 23, 2017 , ... On February 22, 2017 ... letter to withdraw previous guidance issued by the Obama Administration requiring schools ... in May 2016 by the Obama Administration came in response to a growing ...
(Date:2/23/2017)... ... February 23, 2017 , ... The American Cleft Palate-Craniofacial Association (ACPA) is ... KLS is a longtime supporter of the event. , "We are pleased that ... said Dr. Bob Havlik, 2017 ACPA President. "KLS Martin has a long track record ...
(Date:2/23/2017)... ... February 23, 2017 , ... EBSCO Information Services ... Institute for Health and Care Excellence (NICE) framework. The agreement allows purchasers ... (NHS) to search, order and purchase medical and healthcare-related content through GOBI ...
Breaking Medicine News(10 mins):
(Date:2/24/2017)... -- Research and Markets has announced the addition of ... Forecast to 2025" report to their offering. ... The Global Wireless Health Market ... the next decade to reach approximately $330.5 billion by 2025. ... all the given segments on global as well as regional levels ...
(Date:2/24/2017)... Physician General Dr. Rachel Levine ... Jennifer Smith commended South Central EMS today for ... naloxone, a life-saving overdose reversal drug. The recognition event ... and overdose survivor who was saved due to the ... significant part of fighting the opioid epidemic is making ...
(Date:2/24/2017)... 23, 2017 Report analyzes the worldwide markets for ... analytics for the US, Canada , ... Asia-Pacific , Latin America , ... for the period 2015 through 2022. Also, a six-year historic ... are derived from primary and secondary research. Company profiles are ...
Breaking Medicine Technology: