Navigation Links
New test for safer biomedical research results
Date:7/30/2009

In cancer research, as in most other biomedical sciences, they are playing a key role: living cells, kept in sterile plastic containers with red culture media populating incubators in laboratories around the world. But do researchers always know what is really living in their culture dishes? Under the microscope, different cell lines are almost impossible to distinguish from each other. When these important research objects stop growing without apparent reason is it because of the manipulations by the scientists or because of an invisible viral or bacterial infection?

Contaminations with other cell lines or pathogenic agents are a common and well-known problem. Often they are the reason why cell experiments fail to produce useable or reproducible results. Even worse, laboratory staff can get infected with dangerous pathogens from a cell culture.

To make those important cell culture experiments safer, DKFZ researchers Dr. Markus Schmitt and Dr. Michael Pawlita have developed a test which is able to identify 37 different cell contaminations in a single run. The researchers have tested the system in over 700 samples from different research labs and have now published their results.

The method called "Multiplex cell Contamination Test" (McCT) detects not only wide-spread viruses but also a number of mycoplasmas, which are considered the major contaminators of cell cultures. In addition, the test checks the cells for their origin. Thus, if dog genetic material is found in what are supposed to be monkey cells, then a contamination of the cell culture is obvious. The test also includes detection of commonly used standard cell lines. Contamination with the fast-growing cancer cell line HeLa, for example, is a dreaded source of false results.

Pawlita and Schmitt found contaminations in a high percentage of cell samples. Twenty-two percent of tested cultures were contaminated with one of the various types of the parasitic bacterium called mycoplasma. "What we noticed about the results," says Markus Schmitt, "was that contaminations were frequent in some laboratories, while others sent in cultures that were constantly clean. Thus, care in laboratory work seems to play an important role."

The test is highly specific and needs no more than ten copies of foreign DNA in the cell sample to be positive. This is a sensitivity which is comparable to or even higher than those of previously available commercial mycoplasma tests. McCT results are reproducible to 99.6 percent. The method is based on multiplication of specific DNA sequences by polymerase chain reaction and subsequent detection of the multiplied DNA regions. A special advantage of the new test is that it can be carried out on a high-throughput basis. The DKFZ researchers can manage up to 1,000 tests per week.

Schmitt und Pawlita offer the service to external scientists and research institutes via the Steinbeis Transfer Center "Multiplexion", a DKFZ spin-off. If you are interested, please visit www.multiplexion.com for more information about the conditions.


'/>"/>

Contact: Dr. Sibylle Kohlstdt
s.kohlstaedt@dkfz.de
Helmholtz Association of German Research Centres
Source:Eurekalert

Related medicine news :

1. Study Finds Weight-Loss Surgery Safer Than Thought
2. Smokeless tobacco safer than smoking
3. Seven Tips for Safer Home Swimming Pools and Hot Tubs
4. New Anesthesia May Be Safer for Critically Ill
5. Mass. General team develops potentially safer general anesthetic
6. Making the difficult task of transporting a child with special needs safer
7. Prostate Drug Appears Safer Than Thought
8. Consumers are Looking to Boiron Arnicare(R) as a Safer Option as FDA Enforces Stricter Labeling for Many Pain Relievers
9. Mussel-Based Glue May Make Surgery Safer
10. Oh Baby, Interventional Radiology Makes Childbirth Safer
11. New information points to safer methadone use for treatment of pain and addiction
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/13/2017)... New York, NY (PRWEB) , ... October 13, 2017 , ... ... most influential people in business to advocate for action towards gender equality at their ... 18,000 views from around the globe, and reached a social audience of over 3 ...
(Date:10/13/2017)... ... October 13, 2017 , ... Yisrayl Hawkins, Pastor and ... explains one of the most popular and least understood books in the Holy Scriptures, ... puzzling descriptions that have baffled scholars for centuries. Many have tossed it off as ...
(Date:10/13/2017)... ... October 13, 2017 , ... Coveros, a leader in ... awarded a contract by the Center for Medicare and Medicaid Services (CMS). The ... enterprise use of Agile methodologies in a consistent and high value manner across ...
(Date:10/13/2017)... ... ... “America On The Brink”: the Christian history of the United States and ... of published author, William Nowers. Captain Nowers and his wife, Millie, have six ... years in the Navy. Following his career as a naval aviator and carrier ...
(Date:10/12/2017)... (PRWEB) , ... October 12, 2017 , ... ... are now treating sleep apnea using cutting-edge Oventus O2Vent technology. As ... serious sleep disorder characterized by frequent cessation in breathing. Oral appliances can offer ...
Breaking Medicine News(10 mins):
(Date:9/22/2017)... AVACEN Medical (AVACEN) announced that its CE-Marked ... those with the widespread pain associated with fibromyalgia in ... Essex, England commented, "I had ... no sleep at all, tremendous pain, with every movement ... [the AVACEN 100] enough, how this has and is ...
(Date:9/18/2017)... , Sept. 18, 2017 EpiVax, ... of bioinformatics and immune engineering, today announced ... influenza A (H7N9) vaccine. ... to seasonal influenza and presents a challenge ... prior exposure to be effective. Using state-of-the-art ...
(Date:9/12/2017)... SAN FRANCISCO , Sept. 12, 2017 /PRNewswire/ ... Lifecycle Management Solutions (VLMS), is pleased to announce ... as a member of its Board of Directors ... 2017. ValGenesis VLMS enables life science companies to ... eliminate the use of paper in this process. ...
Breaking Medicine Technology: