Navigation Links
New technique permits development of enzyme tool kit

An Arizona State University graduate student, Jinglin Fu, in collaboration with Biodesign Institute researchers Neal Woodbury and Stephen Albert Johnston, has pioneered a technique that improves on scientists' ability to harness and modulate enzyme activity.

The new approach, reported in the Journal of the American Chemical Society (published recently online), could have wide applicability for designing a range of industrial catalysts, health care diagnostics and therapies centered on understanding the control of enzymatic activity.

Enzymes, key catalysts that speed up the reactions inside every cell, are critical for life. As Neal Woodbury, chief scientist the Biodesign Institute at Arizona State University notes, "all the processes that happen inside of your body, essentially without exception, are run by enzymes." Enzymes are also a prized tool in biomedical research, aiding the development of diagnostic tests and therapeutics for a range of human diseases.

But studying the role of enzymes can be tricky. One approach has been to use a specialized platform known as a microarraywhere glass slides are deposited with 10,000 protein fragments, called peptides, that are screened for their ability to react with specific enzymes and alter their activity. "On the microarray, you can screen thousands of molecules at the same time," Fu says, allowing the simultaneous monitoring of the peptide-enzyme binding and the change in enzyme activity at each spot on the array.

But there is a problem with this approach, that has so far hampered enzyme research. "When you try to monitor the chemical reaction that the enzyme catalyzes in the microarray, the molecule generated by the enzyme reaction quickly diffuses away, causing serious cross-contamination between spots on the array," Fu explains. To solve this problem, Fu applied polyvinyl alcohol (PVA)a thick, viscous and clear polymer to the microarray slide to limit the diffusion of molecules and hold the reactions in place, preventing contamination.

In the current study, Fu's team was able to observe the effects that peptides had on the activity of three broad classes of enzymes. In some cases, peptides blocked the activity of an enzyme but in others, peptides acted to alter the whole structure of the enzymeoften in unanticipated waysallowing it to function differently.

"What Jinglin has invented," Woodbury stresses, "is a way of finding a peptide that will allow us to both put an enzyme in a particular place and modulate its activity. It allows us to begin to group different enzymes according to function." In addition to possible biomedical applications, the enzyme tool kit made possible through the group's research could be applied to modulating enzymes for a variety of industrial purposes, for new detergents or pharmaceuticals. Further, the strategy is not limited to peptides. It can theoretically be applied to virtually any small molecule suitable for an array, making the technique extremely versatile.


Contact: Joseph Caspermeyer
Arizona State University

Related medicine news :

1. New Study Demonstrates Novel Use of Metabolic Imaging to Locate Sperm in Infertile Men -- Non-Invasive Imaging Procedure May Replace Invasive Techniques such as Testicula
2. Survey finds many surgeons suffer injuries from minimally invasive techniques
3. Promising new neuroimaging techniques for early detection of Alzheimers disease
4. Sanford Heart Hospital Physicians Again Recognized Internationally for Innovative Techniques
5. Hair Cloning Not Yet Recommended by Researchers Who Developed the Technique
6. New Study: Innovative Technique Allows Male Cancer Survivors Who are Sterile from Treatment to Father Children
7. New measurement technique will help in fight against cancer
8. Shoulder Repair Technique Borrows From Cadavers
9. Researchers Use New Techniques to Assess PTSD
10. Controversial kidney transplant technique could provide lifeline for very ill patients
11. The New Liposuction Technique Means No Surgery or Suction
Post Your Comments:
(Date:6/25/2016)... , ... June 25, 2016 , ... ... and non-athletes recover from injury. Recently, he has implemented orthobiologic procedures as a ... area —Johnson is one of the first doctors to perform the treatment. Orthobiologics ...
(Date:6/25/2016)... ... 2016 , ... Conventional wisdom preaches the benefits of moderation, whether it’s a ... the bar too high can result in disappointment, perhaps even self-loathing. However, those who ... , Research from reveals that behind the tendency to set ...
(Date:6/24/2016)... Michigan (PRWEB) , ... June 24, 2016 , ... Those ... deal with these feelings, many turn to unhealthy avenues, such as drug or alcohol ... of Marne, Michigan, has released tools for healthy coping following a traumatic event. , ...
(Date:6/24/2016)... ... , ... Marcy was in a crisis. Her son James, eight, was out of control. Prone ... physically. , “When something upset him, he couldn’t control his emotions,” remembers Marcy. “If ... at my other children and say he was going to kill them. If we ...
(Date:6/24/2016)... , ... June 24, 2016 , ... Global law firm ... 2016 Legal Elite. The attorneys chosen by their peers for this recognition are considered ... Seven Greenberg Traurig Shareholders received special honors as members of this year’s Legal Elite ...
Breaking Medicine News(10 mins):
(Date:6/26/2016)... -- VMS Rehab Systems, Inc. ( ) reported today ... to build a strong and stable market for trading ... the OTC Markets-pink current trading platform. Explains ... seeing an anomaly in market trading activities that may ... Company, but shareholders and market players as well. I ...
(Date:6/24/2016)... FRANCISCO, Calif. , June 24, 2016 /PRNewswire/ ... ), a biopharmaceutical company developing novel therapeutics for ... unmet needs, today announced the closing of its ... of common stock, at the public offering price ... in the offering were offered by GBT. GBT ...
(Date:6/24/2016)... 24, 2016 The Academy of Managed Care ... that would allow biopharmaceutical companies to more easily ... make formulary and coverage decisions, a move that addresses ... medicines. The recommendations address restrictions in the ... the drug label, a prohibition that hinders decision makers ...
Breaking Medicine Technology: