Navigation Links
New technique catalogs lymphoma-linked genetic variations

As anyone familiar with the X-Men knows, mutants can be either very good or very bad or somewhere in between. The same appears true within cancer cells, which may harbor hundreds of mutations that set them apart from other cells in the body; the scientific challenge has been to figure out which mutations are culprits and which are innocent bystanders. Now, researchers at Johns Hopkins Medicine have devised a novel approach to sorting them out: they generated random mutations in a gene associated with lymphoma, tested the proteins produced by the genes to see how they performed, and generated a catalog of mutants with cancer-causing potential.

"Our goal was to correlate various mutations with potential to promote lymphoma," says Joel Pomerantz, Ph.D., an associate professor in the Johns Hopkins School of Medicine's Institute for Cell Engineering. For the study, to be reported in a January 2013 issue of Molecular and Cellular Biology, Pomerantz and his research team focused on the protein CARD11. CARD11 plays a key role in signaling the presence of infection, which leads infection-fighting white blood cells to grow and divide. Certain mutations can turn CARD11 permanently "on," causing out-of-control cell division that results in cancers called lymphomas, which strike about 75,000 Americans each year.

To find out which genetic mutations would increase CARD11's activity, Pomerantz and his team made copies of the CARD11 gene in a way that made random mutations likely. They then used the faulty copies to make mutant proteins, and tested the ability of those proteins to trigger the signaling reaction that is CARD11's specialty. This let the researchers figure out which mutations increased the protein's activity, and by how much information that can be compared to emerging data about CARD11 mutations found in human lymphomas. "We found that several of the overactive mutations we'd identified have already been found in patients," Pomerantz says.

Noting that CARD11 is part of the NF-κB signaling pathway, a target of some cancer therapies, Pomerantz says the new cataloging technique could lead to more personalized treatment. "We imagine eventually being able to correlate response to a particular therapy with a particular mutation," he says. For now, Pomerantz and his team are delving deeper into what gives the bad CARD11 mutants their special powers, looking for mechanisms to explain how certain changes increase the protein's activity.


Contact: Shawna Williams
Johns Hopkins Medicine

Related medicine news :

1. New MRI technique may predict progress of dementias
2. Detecting malaria early to save lives: New optical technique promises rapid and accurate diagnosis
3. New technique may help severely damaged nerves regrow and restore function
4. New Techniques May Improve Infant Heart Surgery
5. New surgical technique for removing inoperable tumors of the abdomen
6. New technique predictably generates complex, wavy shapes
7. Screening for breast cancer without X-rays: Lasers and sound merge in promising diagnostic technique
8. A marker in the lining of the lungs could be useful diagnostic technique for lung cancer screening
9. New technique could reduce number of animals needed to test chemical safety
10. Technique spots disease using immune cell DNA
11. Noninvasive imaging technique may help kids with heart transplants
Post Your Comments:
(Date:10/13/2015)... Boulder, Colorado (PRWEB) , ... October 13, 2015 ... ... launched the first high-tech foam roller with 11 hours of vibrating power. Tested ... to reduce recovery time and increase athletic performance. , Mark's background in sporting ...
(Date:10/13/2015)... , ... October 13, 2015 , ... The American Institutes ... college campus health staff caring for students who have experienced sexual assault and other ... the White House during the Violence Against Women Act's 21st anniversary. ...
(Date:10/13/2015)... M.A. (PRWEB) , ... October 13, 2015 , ... Sir ... service to the local Boston chapter of Ronald McDonald House Charities® (RMHC®). This ... that has supported Sir Grout of Greater Boston since its inception. , “We believe ...
(Date:10/13/2015)... ... ... "My friend's son suffers from eczema, and he had a horrible habit ... Platteville, Colo. "I came up with this kit as a way to prevent children ... child from rubbing or scratching his or her face. This protects sensitive skin from ...
(Date:10/13/2015)... , ... October 13, 2015 , ... ... anesthesia and pain management services, today announced its partnership with WPC Healthcare ... data from disparate systems and organizes the data into an aggregated data repository ...
Breaking Medicine News(10 mins):
(Date:10/13/2015)... Oct. 13, 2015 Attracting mid- to large-sized medical ... to growing Baltimore into a major ... by the Maryland Technology Enterprise Institute (Mtech) . ... pieces in place to support innovation hubs and a thriving ... Mtech Baltimore. "This study is important because we believe ...
(Date:10/13/2015)... , Oct. 13, 2015  Graduate students ... care and medical research, will soon have the ... patient care – the drug discovery and development ... ) has collaborated with 10 leaders from academic ... "Making Medicines: The Process of Drug Development."  Lilly ...
(Date:10/13/2015)... SAN FRANCISCO , October 13, 2015 ... expected to reach USD 26.8 billion by 2022, according ... Anesthesia and respiratory devices, owing to their capability to ... expected to witness a substantial growth. --> ... reach USD 26.8 billion by 2022, according to a ...
Breaking Medicine Technology: