Navigation Links
New technique boosts protein NMR imaging speeds
Date:2/9/2009

Solid-state nuclear magnetic resonance, or SSNMR, is a valuable tool to image and analyze the chemical makeup of proteins and other biomolecules. But the imaging process is time-consuming and requires large amounts of costly isotope-labeled sample for study.

Yoshitaka Ishii, associate professor of chemistry at the University of Illinois at Chicago, believes he has found a quicker and more efficient approach to using SSNMR called paramagnetic relaxation-assisted condensed data collection, or PACC. Details of the approach are reported in the March issue of Nature Methods (online Feb. 8.)

Ishii and his associates found a way of increasing sensitivity of SSNMR by doping samples at varying concentrations with the paramagnetic copper-acid solution Cu-EDTA, a chemical used in many industrial applications. That made the study samples more active transponders, providing strong signals and detailed spectral information with minimal downtime.

"With SSNMR, we collect the signal responses but then have to wait for the SSNMR system to recycle, which takes up to three seconds," said Ishii. "You have to do this hundreds of times. And during most of that time, you're basically doing nothing. By our approach, we've reduced that waiting period by up to 20 times."

Ishii said the slow process of gathering spectral signal information has been the "de facto standard for over 20 years." He found it shocking how much time is spent just waiting for results using traditional SSNMR.

The chemists also boosted the SSNMR efficiency by using a spinning speed of 40 kilohertz, instead of the usual 10 kHz, and doing a fast recycling of low radio frequency field power sequences, which minimizes the amount of irradiation heat surrounding the study sample.

"The radio frequency irradiation process typically increases temperature, but we worked it so we could get a signal without strong irradiation, which could fry out a protein," said Ishii.

Ishii and his group studied various types of molecules using this new approach, including the amyloid fibrils often associated with Alzheimer's disease, larger globular proteins and cytoskeleton proteins. The new approach worked well with each type.

The doping solution they added to enhance the sensitivity of samples did not change the chemical structure of proteins studied. Ishii said the approach also enabled his group to get useful spectral signals using much smaller samples.

"We often need samples as large as 10 microliters, but with this approach we can use as little as one microliter or less," he said. "With protein structure work, preparing samples is a major bottleneck, which limits our ability to analyze it. This approach opens up the possibility for more difficult structure determination work."

Ishii hopes the PACC approach may be enhanced to achieve even greater SSNMR sensitivity, but notes the technique, as presently tested, should allow study of molecular structural features that are currently difficult to obtain using other laboratory methods.


'/>"/>

Contact: Paul Francuch
francuch@uic.edu
312-996-3457
University of Illinois at Chicago
Source:Eurekalert

Related medicine news :

1. Surgical technique in Spain enables extirpation of benign tumors of the pancreas
2. ISU researchers develop technique for quick detection of Salmonella
3. 2nd Annual AboutFACE 2009 at The Encore at Wynn Las Vegas Features Leading Instructors and Best Techniques For Practice Success
4. Latest Facelifting Techniques Include Fat Repositioning to Deliver More Youthful, Natural Results
5. From outer space to the eye clinic: New cataract early detection technique
6. Breathing life into injured lungs: World-first technique will expand lung donor organ pool
7. Rochester Shoulder Surgeon Blends Innovative Techniques with Personalized Patient Care
8. Light shines for potential early cancer diagnosis technique
9. New technique enables faster genetic diagnosis for hereditary diseases
10. New bone implant technology using techniques normally used to make catalytic converters
11. Washington, DC Plastic Surgeon Pioneers New Minimal-Scarring Technique
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... ... June 27, 2016 , ... Quality metrics are proliferating ... many ways they remain in the eye of the beholder, according to experts who ... of The American Journal of Managed Care. For the full issue, click here ...
(Date:6/26/2016)... ... June 26, 2016 , ... PawPaws brand pet supplements ... was developed to enhance the health of felines. The formula is all-natural and is ... herbs in the PawPaws Cat Kidney Support Supplement Soft Chews are Astragalus ...
(Date:6/25/2016)... D.C. (PRWEB) , ... June 25, 2016 , ... ... discuss health policy issues and applications at AcademyHealth’s Annual Research Meeting June 26-28, ... their work on several important health care topics including advance care planning, healthcare ...
(Date:6/25/2016)... ... 2016 , ... "With 30 hand-drawn hand gesture animations, FCPX users can easily ... of Pixel Film Studios. , ProHand Cartoon’s package transforms over 1,300 hand-drawn pictures ... . Simply select a ProHand generator and drag it above media or text in ...
(Date:6/25/2016)... Oklahoma City, Oklahoma (PRWEB) , ... June 25, ... ... to helping both athletes and non-athletes recover from injury. Recently, he has implemented ... for the Oklahoma City area —Johnson is one of the first doctors to ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... , June 24, 2016   Bay ... Rehabilitation Network,s Dean Center for Tick Borne ... Medicine and Rehabilitation, MIT Hacking Medicine, University of ... Innovation, today announced the five finalists of ... Lyme disease.  More than 100 scientists, clinicians, researchers, ...
(Date:6/24/2016)... , June 24, 2016 ... the "Structural Electronics 2015-2025: Applications, Technologies, Forecasts" ... In-Mold Electronics, Smart Skin, Structural ... Structural electronics involves electronic and/or ... protective structures, replacing dumb structures such as vehicle ...
(Date:6/24/2016)... , June 24, 2016 Dehaier Medical Systems ... "Company"), which develops, markets and sells medical devices and ... , signed a strategic cooperation agreement with Hongyuan Supply ... Supply Chain") on June 20, 2016, to develop Dehaier,s ... strategic cooperation agreement, Dehaier will leverage Hongyuan Supply Chain,s ...
Breaking Medicine Technology: