Navigation Links
New research finds major activation themes in denture-stomatitis
Date:7/15/2010

Barcelona, Spain Today during the 88th General Session & Exhibition of the International Association for Dental Research, in Barcelona, Spain, S. Offenbacher will present an abstract titled "Mucosal Gene Expression and Salivary Proteomic Analysis of Candidiasis-Associated Denture-Stomatitis."

The goal of the study was to compare whole-transcriptome, mucosal gene expression in Candida albicans (a parasitic fungus that can infect the mouth) associated chronic denture stomatitis to that of healthy oral mucosa and perform proteomic analyses of potential salivary biomarkers. Denture stomatitis is a condition in which the mucosa underneath a denture becomes inflamed and sometimes painful.

In this study, oral palatal biopsies were obtained from 17 healthy and 15 C. albicans-infected stomatitis subjects for whole-transcriptome analyses using Affymetrix arrays. The presence of C. albicans was confirmed by cytology and cultivable methods and the clinical severity of the stomatitis and denture fit evaluated by the Newton and Kapur Classifications.

A false discovery rate (FDR) of <0.05 was chosen and expression pathways associated with stomatitis were identified using Ingenuity Pathway Analysis.

Saliva was analyzed by two-dimensional liquid chromatography and tandem mass spectrometry for proteomic identification of salivary proteins associated with oral Candidiasis.

Microarray analysis of mRNA expression revealed 3034 genes-in-play differentially expressed in C. albicans stomatitis. 235 genes were upregulated >2-fold including key cytokines [IL1F6, IL1B], chemokines [CXCL1, CCL10, IL8] as well as markers of epithelial suppression and neutrophil recruitment/ activation. Seventy-one genes were down-regulated >2-fold including epithelial adhesion molecules and keratins. Five of the 6 most significant gene ontology pathways involve inflammation and activation of the immune response with CD28 and CTLA signaling of Tcells. There was strong up-regulation of TLR2, CD14, MYD88, IKKA and NFKB as the dominant toll-like receptor signaling pathway. Six extracellular protein genes up-regulated in stomatitis were confirmed within the saliva using proteomic methods.

Neutrophil recruitment activation, epithelial suppression, TLR2 pathway up-regulation, T cell activation and bone resorption appear as major activation themes in stomatitis.


'/>"/>

Contact: Ingrid L. Thomas
ithomas@iadr.org
International & American Association for Dental Research
Source:Eurekalert

Related medicine news :

1. UC San Diego researchers find cause of metabolic disease -- and possible cure
2. Researchers cut years from drug development with nanoscopic bead technology
3. New research explores relief for xerostomia cancer patients
4. 5 awarded ASTRO research grants
5. Lu receives grant from Research to Prevent Blindness organization
6. NIH expands food allergy research program
7. VCU first Virginia institution to join national network of academic research centers
8. Researcher targets gene regulators on link between arsenic, cancer
9. Researchers study relationship of oral cancers and periodontal disease
10. Report proposes new research agenda on pregnancy intentions of HIV-positive women
11. Alzheimers Research Spotlights Protein Tangles in Brain
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/24/2017)... Estates, CA (PRWEB) , ... January 24, 2017 , ... ... at studies, work, athletics or even in relationships. Course offered by Dr. Carol ... is necessary due to limited seating and the many hands-on experiences. , ...
(Date:1/24/2017)... PA (PRWEB) , ... January 24, 2017 , ... His ... but the co-founder of the organization End Distracted Driving , Joel Feldman, has ... , Feldman's story was featured in one of the top newspapers in circulation in ...
(Date:1/24/2017)... (PRWEB) , ... January 24, 2017 , ... The University ... has taken great care to design a program that provides teachers with the specific ... domestically. This online teaching certificate is ideal for post-baccalaureate students and HigherEducation.com is working ...
(Date:1/24/2017)... (PRWEB) , ... January 24, 2017 , ... American Family ... Bedford, expanding access to high-quality health care for residents in the area. The Bedford ... location opened during the past four years by Dave Adams and Dr. Kristina Orio. ...
(Date:1/24/2017)... ... January 24, 2017 , ... “Choosing Wisdom-Solomon’s ... finances, friendship, marriage, leadership, gossip, prostitution, adultery, anger, and common sense. , ... Judith Coats and Dr. David Coats. In September of 1983, they flew to ...
Breaking Medicine News(10 mins):
(Date:1/24/2017)... The Society for Non OR Intervention and Anesthesia ( ... Safety ( PPAHS ) are pleased to announce their ... for patients undergoing procedures outside of the Operating Room. ... Michael Wong JD, Executive Director of PPAHS have each ... Boards. SONORIA is an integrated group of ...
(Date:1/24/2017)... Md. , and GAITHERSBURG, Md. , ... XON ), a leader in the engineering and industrialization ... of the planet, today announced that it has entered into ... GNVC ), a clinical-stage company and pioneer in ... Intrexon intends to integrate and expand upon GenVec,s expertise in ...
(Date:1/24/2017)... , January 24, 2017 www.Financialbuzz.com ... growing industries in the United States ... in 2016. In addition, Proposition 64, the California Cannabis Legalization ... 42.87% against on November 8, 2016. This outcome means that ... use and to grow a certain amount of cannabis for ...
Breaking Medicine Technology: