Navigation Links
New options for ease and accuracy in extraction of rare cells or separating blood
Date:10/26/2012

At the Sixteenth International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS) to be held Oct. 28-Nov. 1, in Okinawa, Japan, University of Cincinnati researchers will present four papers, including one detailing improvements in rare cell isolation and one detailing improvements, in terms of cost and time, of common blood tests.

Ian Papautsky, associate professor in UC's School of Electronic and Computing Systems (SECS), part of the College of Engineering and Applied Science, and a UC team are leading these research efforts.

In a paper titled "Continuous Rare Cell Extraction Using Self-Releasing Vortex in an Inertial Microfluidic Device" by Papautsky and co-authors Xiao Wang, UC doctoral student, and Jian Zhou, research associate, a new concept for separation of rare cells, such as prostate cancer cells or circulating tumor cells, using microfluidics, is detailed.

"Last year we showed we can selectively isolate prostate cancer cells, but only by running small sample volumes one at a time. Now we show that we can do this continuously," Papautsky said. "This is exciting because it allows for an entire blood draw to be processed, in continuous matter, in a shorter period of time."

These blood draws can be used to identify tumor cells for diagnostic or prognostic purposes. "Our approach is based purely on size. It doesn't rely on antibodies, which is important because not all cancer cells express antigens. So, if the cancer cells are, let's say, larger than 20 microns, we'll extract them," he explained.

The most common approach for looking for these circulating tumor cells is via a system that uses a selection using antibodies to detect antigens. "We could also use our device to prepare samples for systems that use antibody-based selection." This combined approach could potentially help reduce occurrence of false positives while significantly increasing the accuracy of the antibody-based tests.

Another area in which this device could be useful is in working with cell cultures. "If you have a mixture of multiple cells where some cells are small and other cells are big, we could separate these cell populations very easily," Papautsky explained. "Anytime you need to separate based on size, we can do it using inertial microfluidics."

The advantage of inertial microfluidics in cell separation is that it can be done easily and without cumbersome equipment. This research is leading to an entirely new generation of testing capabilities which particularly lend themselves to direct use in the field and in physicians' offices in just about any country and any economic setting.

In another paper, titled "Sorting of Blood in Spiral Microchannels" Papautsky and doctoral student Nivedita Nivedita demonstrate continuous sorting of blood utilizing inertial microfluidics via a simple passive microfluidic device. Papautsky's lab has been developing the concept of using inertia to manipulate cells and particles during the last few years. "It's truly different and innovative because these microfluidic devices are really low cost while offering very high throughput," said Papautsky.

The device is, essentially, a clear, plastic, flexible square that is relatively small in size, at about a half an inch across, but big in concept. "With this particular device we can take a drop of blood, put it in the input port in the center, and separate," Papautsky explained. The device contains four outlet ports which separate the blood into different streams, allowing the collection of outputs containing dilute plasma, red blood cells and white blood cells.

"There are a lot of clinical diagnostic tests that are based on blood," he said. One of the most common tests that are done in a hospital is the complete blood count (CBC). Through this test, a wide range of conditions like anemia, malaria or leukemia are diagnosed. "In all of these diagnostic tests, blood must be separated into its components, and that's what this device does," Papautsky explained. "So, instead of using a big centrifuge to do it, we can do it with this little device." Using the microfluidic device allows for a diagnosis in less time in a much easier fashion.

This quick, low-cost way of running a diagnostic test could potentially be used in a resource-limited setting. "One of the issues that I hear from my colleagues who work in these areas that do tests is that they have equipment," he said, "but don't always have personnel or stable power to operate them. So in places like India, Africa or Central America, our devices could be useful."


'/>"/>
Contact: M.B. Reilly
reillymb@ucmail.uc.edu
513-556-1824
University of Cincinnati
Source:Eurekalert  

Related medicine news :

1. Version 2.0 of Award-Winning Proloquo2Go App for iPhone, iPad & iPod touch Speeds Up Communication, Adds New Voices and Customization Options
2. Mesothelioma Victims Center Now Urges US Navy Veterans Or Individuals With Mesothelioma To Call Them For The Names Of The Best Mesothelioma Attorneys & Treatment Options
3. Menu labeling requirements lead to healthier options at chain restaurants
4. Options Increasing for Coping With Kids Food Allergies
5. Existing drugs offer new treatment options for high-risk childhood leukemia subtype
6. Many options, good outcomes, for early-stage follicular lymphoma
7. Little evidence supports autism treatment options in adolescents
8. Revised geographic adjustments could improve accuracy of Medicare payments, will not solve access, quality problems
9. Unprecedented accuracy in locating brain electrical activity with new device
10. Accuracy of narrow band imaging with colonoscopy allows for distal non-cancerous polyps to be left in place
11. New drug prevents spread of human prostate cancer cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New options for ease and accuracy in extraction of rare cells or separating blood
(Date:1/20/2017)... (PRWEB) , ... January 20, 2017 , ... “Code Word: Chocolate Biscuit”: a biographical account ... Word: Chocolate Biscuit” is the creation of published author, Marlyn Ivey, born in Lynn Haven, ... he went to school and at 19 years of age, he joined the Navy and ...
(Date:1/20/2017)... ... January 20, 2017 , ... ... Oscillating Positive Expiratory Pressure (OPEP) device, was featured in a study indicating superior ... MEd, RRT-ACCS, FAARC, “Analysis of Three Oscillating Positive Expiratory Pressure Devices During ...
(Date:1/20/2017)... ... ... to Christmas:” a beautiful and enchanting tale that teaches children the true meaning of Christmas. ... in Oklahoma City, and a devoted woman of faith. , “Becoming a parent changes ... back of my mind for years, but actually doing it might have been a while ...
(Date:1/20/2017)... (PRWEB) , ... January 20, 2017 , ... “The Land ... brings attention to the issue of world hunger, and shares the simple and achievable ... Brubaker, devoted husband and member of the Fairview Missionary Church in Angola, Indiana where ...
(Date:1/19/2017)... ... January 20, 2017 , ... Today, the Centers for Medicare & Medicaid ... CMS’s Alternative Payment Models (APMs) in 2017. Clinicians who participate in APMs are paid ... important part of the Administration’s effort to build a system that delivers better care ...
Breaking Medicine News(10 mins):
(Date:1/20/2017)... Wells Specialty Pharmacy announces the acquisitions and merger ... Winter Park, Florida and Pharm-EZ Medical, ... have been consolidated into the 3796 Howell Branch Road Facility. ... that Chad Tomlinson , former Vice President of Operations, ... Mr. Tomlinson is a Graduate of Florida State University and ...
(Date:1/19/2017)... 2017 Report Details What can ... are going to grow at the fastest rates? This ... data, trends, opportunities and prospects. Our 190-page report ... lucrative areas in the industry and the future market ... across the all the major categories of the ophthalmic ...
(Date:1/19/2017)... This report on the opioid induced constipation ... the global market. Large number of chronic pain sufferers ... is a major side effect of consumption of opioid ... therapy has been prescribed to treat opioid induced constipation. ... and growing awareness about the therapy are the major ...
Breaking Medicine Technology: