Navigation Links
New microscopy tracks molecules in live tissue at video rate

CAMBRIDGE, Mass. -- A novel type of biomedical imaging, made possible by new advances in microscopy from scientists at Harvard University, is so fast and sensitive it can capture "video" of blood cells squeezing through capillaries.

Researchers led by Harvard's Brian G. Saar, Christian W. Freudiger, and X. Sunney Xie describe the work this week in the journal Science.

The new technique, based on stimulated Raman scattering (SRS), makes a complementary partner to MRI, widely used to capture static images of organs, tumors, and other large structures. For the first time, SRS microscopy makes possible label-free chemical movies, with streaming footage at the subcellular level, catching video of proteins, lipids, and water within cells.

"When we started this project 11 years ago, we never imagined we'd have an amazing result like this," says Xie, professor of chemistry and chemical biology at Harvard. "It took MRI more than 30 years to reach patients, but we're already looking forward with great anticipation to applications of SRS microscopy in hospitals. It's now clear that stimulated Raman scattering will play an important role in the future of biological imaging and medical diagnostics."

Xie says SRS microscopy could aid, and speed, surgery to remove tumors and other lesions. Surgeons must now send excised samples for histological analysis -- a process that takes about 20 minutes -- while a patient waits on the operating table. SRS microscopy provides equivalent insights through real-time scanning.

Xie's team has already used SRS microscopy to track migration of medications in skin, shedding new light on the absorption of topical drugs. In conjunction with endoscopy, the technique can also view three-dimensional sections of tissue, layer by layer.

"Previous SRS microscopy captured only about one image per minute, far too slow for use in live animals or humans," Xie says. "We were able to speed the collection of data by more than three orders of magnitude, attaining video-rate imaging."

Because SRS microscopy works by detecting the intrinsic vibrations in chemical bonds between atoms, it doesn't require intrusive fluorescent labeling. An optical technique, it compliments MRI, whose depth of penetration is better suited to imaging organs and other large objects deep within the body.

The current work greatly improves detection of signals -- backscattered by tissues in the body -- by rearranging photodetectors to surround a small aperture through which a beam of light is directed at the tissue being examined. Using this approach, the scientists were able to collect and analyze almost 30 percent of the laser light directed at a biological sample, a more than 30-fold increase over previous SRS microscopy.

Scientists currently use a variety of techniques to view biomolecules, but most have significant limitations that are sidestepped by SRS microscopy. Labeling with green fluorescent protein (GFP) provides sharp images, but the bulky protein can perturb delicate biological pathways, overwhelming smaller biomolecules. Conventional infrared (IR) microscopy has low spatial resolution and requires desiccated samples, while spontaneous Raman microscopy requires high laser power and long integration times, limiting use in live specimens. Coherent anti-Stokes Raman scattering (CARS) microscopy, pioneered by Xie's own group, lacks the contrast to image most molecules beyond lipids.


Contact: Steve Bradt
Harvard University

Related medicine news :

1. Hayes, Inc. Technology Prognosis Team Tracks First-of-a-Kind Technologies
2. Fit People Release More Fat-Burning Molecules During Exercise
3. Exploring the Davids and Goliaths of therapeutic molecules
4. New Cancer-Fighting Strategy Focuses On Signaling Molecules
5. Tiny molecules may tell big story about cardiovascular disease risk
6. Nerve Tissue Cancer More Often Fatal for Non-White Kids
7. Stanford researchers first to turn normal cells into 3-D cancers in tissue culture dishes
8. Scientists develop method to keep surgically-removed prostate tissue alive and working for week
9. IU researchers: Chemotherapy alters brain tissue in breast cancer patients
10. USGI medical tissue anchors show durability beyond 1 year
11. Natural lung material is promising scaffold for engineering lung tissue using embryonic stem cells
Post Your Comments:
Related Image:
New microscopy tracks molecules in live tissue at video rate
(Date:10/13/2017)... Vegas, Nevada (PRWEB) , ... October 13, 2017 , ... ... 7® Hemp CBD Oil utilizing Purzorb™ technology. Applying the Purzorb™process to full spectrum CBD ... dose required and providing a CBD form that can be easily incorporated into liquid ...
(Date:10/13/2017)... ... October 13, 2017 , ... The Visiting Nurse Association (VNA) of Somerset ... of specialty vendors and unique items from across the nation, this holiday-themed event will ... services offered by the VNA. The boutique will be open Saturday, November 4 ...
(Date:10/13/2017)... Fairfax, VA (PRWEB) , ... October 13, 2017 ... ... provider of DevOps and Agile Software Development, has been awarded a contract by ... (EATS) Blanket Purchase Agreement (BPA) aims to accelerate the enterprise use of Agile ...
(Date:10/12/2017)... (PRWEB) , ... October 12, 2017 , ... Planet Fitness, ... the U.S., announced today its plans to open a flagship location in Covington, LA ... the former Rooms To Go store next to Office Depot in the Holiday Square ...
(Date:10/12/2017)... Maryland (PRWEB) , ... October 12, 2017 , ... ... magnetic drug delivery system that we intend to develop to enable prevention of ... can lead to severe hearing loss, especially in pediatric patients. For cisplatin, hearing ...
Breaking Medicine News(10 mins):
(Date:10/10/2017)...  NDS received FDA 510(k) clearance in May 2017 for its ... specifically designed for endoscopy environments. An innovative secondary monitor solution, ZeroWire ... to support the improvement of patient outcomes, procedural efficiency, and the ... ... ...
(Date:10/5/2017)... 5, 2017  In response to the nationwide ... Maxillofacial Surgeons (AAOMS) released prescribing recommendations that urge ... used as a first-line therapy to manage a ... Recognizing the value and importance ... "Opioid Prescribing: Acute and Postoperative Pain Management" stresses ...
(Date:10/4/2017)... OBP Medical , a leading ... today announced regulatory approval from Brazil,s ... Nacional de Vigilância Sanitária (ANVISA)) to market ... with integrated LED light source and smoke evacuation ... of a tissue pocket or cavity during surgical ...
Breaking Medicine Technology: