Navigation Links
New microchip technology performs 1,000 chemical reactions at once
Date:8/3/2009

Flasks, beakers and hot plates may soon be a thing of the past in chemistry labs. Instead of handling a few experiments on a bench top, scientists may simply pop a microchip into a computer and instantly run thousands of chemical reactions, with results literally shrinking the lab down to the size of a thumbnail.

Toward that end, UCLA researchers have developed technology to perform more than a thousand chemical reactions at once on a stamp-size, PC-controlled microchip, which could accelerate the identification of potential drug candidates for treating diseases like cancer.

Their study appears in the Aug. 21 edition of the journal Lab on a Chip and is currently available online.

A team of UCLA chemists, biologists and engineers collaborated on the technology, which is based on microfluidics the utilization of miniaturized devices to automatically handle and channel tiny amounts of liquids and chemicals invisible to the eye. The chemical reactions were performed using in situ click chemistry, a technique often used to identify potential drug molecules that bind tightly to protein enzymes to either activate or inhibit an effect in a cell, and were analyzed using mass spectrometry.

While traditionally only a few chemical reactions could be produced on a chip, the research team pioneered a way to instigate multiple reactions, thus offering a new method to quickly screen which drug molecules may work most effectively with a targeted protein enzyme. In this study, scientists produced a chip capable of conducting 1,024 reactions simultaneously, which, in a test system, ably identified potent inhibitors to the enzyme bovine carbonic anhydrase.

A thousand cycles of complex processes, including controlled sampling and mixing of a library of reagents and sequential microchannel rinsing, all took place on the microchip device and were completed in just a few hours. At the moment, the UCLA team is restricted to analyzing the reaction results off-line, but in the future, they intend to automate this aspect of the work as well.

"The precious enzyme molecules required for a single in situ click reaction in a traditional lab now can be split into hundreds of duplicates for performing hundreds of reactions in parallel, thus revolutionizing the laboratory process, reducing reagent consumption and accelerating the process for identifying potential drug candidates," said study author Hsian-Rong Tseng, a researcher at UCLA's Crump Institute for Molecular Imaging, an associate professor molecular and medical pharmacology at the David Geffen School of Medicine at UCLA, and a member of the California NanoSystems Institute at UCLA.

Kym F. Faull, director of the Pasarow Mass Spectrometry Lab at UCLA, helped the team with several challenges, including reducing the amount of chemicals needed for reactions on the chip, enhancing test sensitivity and speeding up reaction analysis.

"The system allows researchers to not only test compounds quicker but uses only tiny amounts of materials, which greatly reduces lab time and costs," said Faull, a professor of psychiatry and biobehavioral sciences at the Geffen School of Medicine.

Next steps for the team include exploring the use of this microchip technology for other screening reactions in which chemicals and material samples are in limited supply for example, with a class of protein enzymes called kinases, which play critical roles in the malignant transformation of cancer.

According to the researchers, the technology may open up many areas for biological and medicinal study.


'/>"/>

Contact: Rachel Champeau
rchampeau@mednet.ucla.edu
310-794-2270
University of California - Los Angeles
Source:Eurekalert  

Related medicine news :

1. Microchip-based device can detect rare tumor cells in bloodstream
2. Microchip Spots Stray Tumor Cells in the Bloodstream
3. Ezra Van Gelder Joins Microchip Biotechnologies as Executive Vice President R&D
4. Quantum light beams good for fast technology
5. New Smart Technology Makes Patient Data Collection Easier, More Efficient
6. Align Technology Executive to Present at Upcoming Financial Conferences
7. Pioneer Surgical Technology Hires Vice President of New Business Development
8. Purdue researchers develop technology to detect cancer by scanning surface veins
9. Creative Technology Services Enhances its Global Competitiveness with Additional Regulatory Certifications
10. University of Wisconsin Hospitals and Clinics Complete System-Wide Conversion to Masimo SET Pulse Oximetry Technology
11. Patent Reform Legislation Threatens Americas Leadership in Medical Technology Innovation
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New microchip technology performs 1,000 chemical reactions at once
(Date:10/13/2017)... TX (PRWEB) , ... October 13, 2017 , ... ... planning agency serving communities in the greater Dallas, Miami, and Raleigh regions, is ... boy fighting to overcome a rare and deadly chromosome abnormality. , After struggling ...
(Date:10/13/2017)... ... 13, 2017 , ... As health professionals work to improve their approach to ... is doing more than filling out a survey; in many cases health professionals and ... in health care and research on the importance of active engagement with patients and ...
(Date:10/13/2017)... Ky. (PRWEB) , ... October 13, 2017 , ... The ... MPH to become its next President and Chief Executive Officer, succeeding Dr. James C. ... CEO Elect beginning July 1, 2018 until Dr. Puffer’s retirement at the end of ...
(Date:10/13/2017)... Vegas, Nevada (PRWEB) , ... October 13, 2017 , ... ... 7® Hemp CBD Oil utilizing Purzorb™ technology. Applying the Purzorb™process to full spectrum CBD ... dose required and providing a CBD form that can be easily incorporated into liquid ...
(Date:10/13/2017)... , ... October 13, 2017 , ... Lori R. Somekh, ... member of ElderCounsel, a national organization of elder law and special needs planning attorneys. ... and rules. It also provides a forum to network with elder law attorneys nationwide,” ...
Breaking Medicine News(10 mins):
(Date:9/28/2017)... Cohen Veterans Bioscience and Early Signal Foundation announce a ... sensors for real-time monitoring of patients with trauma-related and ... focused on disruptive health solutions for rare disorders and ... record and integrate behavioral, cognitive, physiological and contextual data. ... ...
(Date:9/25/2017)... Sept. 25, 2017  EpiVax, Inc., a leader ... and immune-engineering today announced the launch of EpiVax ... of personalized therapeutic cancer vaccines. EpiVax has provided ... access to enabling technologies to the new precision ... lead EpiVax Oncology as Chief Executive Officer. Gad ...
(Date:9/19/2017)... 19, 2017 HistoSonics, Inc., a venture-backed medical device company developing a non-invasive, robotically ... announced three leadership team developments today:   ... ... Tom ... Veteran medical device executive Josh Stopek , PhD, who ...
Breaking Medicine Technology: