Navigation Links
New method of tissue banking makes gene analysis more practical for lung cancer
Date:7/15/2010

Analyzing the genes expressed by cancer cells allows for a better understanding of that patient's specific disease and in turn, a more personalized approach to treatment. But obtaining the RNA from a tumor in the lungs in order to conduct the genetic analysis is a challenging prospect. Currently, lung cancer researchers are limited to using RNA extracted from early-stage tumors removed during surgery. The small quantities of tissue extracted during routine diagnostic biopsies have not been useful to researchers, due to their small size and the variety of ways they have been processed.

Since oftentimes surgery is not an option in advanced lung cancer, genetic analysis of the tumor is critical, there is a need to obtain good quality RNA samples from tumor tissue taken via biopsy, no matter how the biopsy procedure is conducted.

In a study in the July edition of the Journal of Thoracic Oncology, Malcolm H. Lawson describes a process to successfully obtain and to store high quality RNA from lung tumor biopsies. Lawson and fellow researchers at the Cancer Research UK Cambridge Research Institute and Papworth Hospital, both in the United Kingdom, received consent from patients to take extra biopsies for research purposes during the diagnostic procedure. Biopsies were obtained using the three most frequently used techniques -- endobronchial biopsy forceps, transbronchial needle aspirate - or CT-guided needle biopsy. Acceptable RNA for gene expression analysis was extracted from 72 percent of lung cancer biopsies.

Lawson and team immediately froze some of the biopsies in liquid nitrogen, while the others were first treated with an RNA preservative (RNAlater) before freezing. Use of the RNA preservative resulted in higher quality, more intact RNA from biopsies taken via all methods.

Storage in the RNA preservative doubled the number of biopsies that met the minimum yield and quality criteria for analysis compared with immediate freezing (10 of 16 biopsies vs. 5 of 16). Using the RNA preservative, 70 percent of biopsies taken by needle aspiration were suitable for analysis compared with 40 percent frozen, and 50 percent of the RNA preservative endobronchial biopsies were acceptable compared with 17 percent of the frozen ones. A full 100 percent of the CT-guided needle biopsies met the criteria when treated with RNA preservative.

"This is a novel model for tissue banking lung cancer diagnostic specimens that greatly increases the number and type of tumors available for gene expression studies," Dr. Lawson said. "If used widely, it makes personalized medicine for lung cancer patients a more practical proposition."


'/>"/>

Contact: Megan Richter
mrichter@spectrumscience.com
202-587-2556
International Association for the Study of Lung Cancer
Source:Eurekalert

Related medicine news :

1. New UC Davis study finds early Alzheimers identification method
2. Principal Protected Index Investing Provides Innovative Method to Significantly Reduce Stock Market Loss and Risk
3. New Method Proposed for Gauging Hospital Infection Rates
4. Delivery Method May Determine the Bacteria Babies Acquire
5. Violence, not overdose, the likely method of suicide in veterans with substance use disorders
6. Gene-Based Detection Method Might Spot HIV Earlier
7. New method manipulates particles for sensors, crime scene testing
8. New treatment method safe, effective for advanced melanoma patients
9. Color-coded tracking method helps scientists analyze outcomes of newly transplanted tissue
10. OHM Cheers End of the "Build it Bigger" Directive: Metropolitan Communities Look to New Methods to Evaluate, Rehabilitate Aging Sewer Systems
11. PMSI Advocacy Efforts Prompt CMS to Improve MSA Review Methodology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/29/2017)... CT (PRWEB) , ... March 29, 2017 , ... HealthCareMandA.com ... —on Thursday, April 20, 2017, at 1:00 PM ET. A recording of the webinar ... the Interactive Webinar Series. , Home health and hospice companies are still popular targets ...
(Date:3/29/2017)... ... March 29, 2017 , ... Immunotherapy has emerged as one ... cancers and is touted to be the next revolution in our fight against this ... in the form of immune checkpoint inhibitors such as PD-1 and PD-L1 inhibitors. , ...
(Date:3/29/2017)... PA (PRWEB) , ... March ... ... eastern suburbs and South Hills of Pittsburgh now have easier access to ... Pennsylvania only by Allegheny Health Network (AHN). Orthopaedic surgeons at Forbes Hospital ...
(Date:3/29/2017)... ... ... The Wharton School of the University of Pennsylvania is pleased to announce that ... gift to establish the Ken Moelis and Julie Taffet Moelis Advance Access Program ... for highly-qualified Penn undergraduates whose academic and career interests expand traditional notions of business ...
(Date:3/29/2017)... ... March 29, 2017 , ... VisualSP has helped over 1.5 million SharePoint ... option for on-premises installation of its Help System for SharePoint was at the farm ... into the entire tenant. , The company recently released a modified version of ...
Breaking Medicine News(10 mins):
(Date:3/29/2017)... , Mar. 29, 2017 Research and Markets has ... Policy, Patients Treated at Proton Therapy Centers & Forecast" report to ... The South ... its current market size in 2016. The Untapped Proton Therapy Market for ... Billion USD in 2016. Proton Therapy plays an important ...
(Date:3/29/2017)... , Mar 29, 2017  Providence Medical ... announced the addition of two industry veterans to ... has joined the company to lead Global Marketing, ... leadership.  Together, Mr. Lynch and Mr. Scott have ... Mr. Lynch joins the company with ...
(Date:3/29/2017)...  Zynex (OTCQB: ZYXI), an innovative medical technology company specializing ... for pain management, stroke rehabilitation, cardiac monitoring and neurological ... 2016 full-year investor webcast on Monday, April 3, 2017 at 9:00 a.m. ... expects to file its 2016 full year financial results ... ...
Breaking Medicine Technology: