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New buffer resists pH change, even as temperature drops
Date:1/14/2008

t lower temperatures while others grew more acidic.

These observations led to an obvious methodology: Why dont we just mix them together" Sieracki said.

Little by little, he varied the proportions of the combined buffers until he found a formula that exhibited minimal pH changes at a variety of temperatures. Instead of registering changes of 2 or more pH units while cooling, which was typical of some standard buffers, the new formula changed less than 0.2 pH units during cooling, he said.

Were canceling out 100-fold changes in proton concentration and bringing them down within an order of magnitude, Sieracki said.

The creation of a temperature-independent pH (TIP) buffer could have broad implications for new and previously published research, Lu said.

Were not in the business of looking at the literature and correcting other mistakes, he said. But some of the conclusions from previous studies could be on shaky ground if a buffer was used that changed pH dramatically at low temperatures.

The new buffer is immediately useful for biological research, and Sieracki said he is confident that a similar buffer could be made for use in many fields, such as biochemistry, biophysics, chemical biology and biomedical research.


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Contact: Diana Yates
diya@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

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