Navigation Links
New buffer resists pH change, even as temperature drops
Date:1/14/2008

Researchers at the University of Illinois have found a simple solution to a problem that has plagued scientists for decades: the tendency of chemical buffers used to maintain the pH of laboratory samples to lose their efficacy as the samples are cooled. The research team, headed by chemistry professor Yi Lu, developed a method to formulate a buffer that maintains a desired pH at a range of low temperatures.

The study appears this month in Chemical Communications.

Scientists have known since the 1930s that the pH of chemical buffers that are used to maintain the pH of lab samples can change as those samples are cooled, with some buffers raising and others lowering pH in the cooling process.

Freezing is a standard method for extending the shelf life of biological specimens and pharmaceuticals, and biological samples are routinely cooled to slow chemical reactions in some experiments. Even tiny changes in the acidity or alkalinity of a sample can influence its properties, Lu said.

We like to freeze proteins, nucleic acids, pharmaceutical drugs and other biomolecules to keep them a long time and to study them more readily under very low temperatures using different spectroscopic techniques and X-ray crystallography, Lu said. But when the pH changes at low temperature, the sample integrity can change.

Graduate student Nathan Sieracki demonstrated this by repeatedly freezing and thawing oxacillin, a penicillin analog used to treat infections.

After one freeze-thaw 50 percent of the drug was dead in several of the buffers investigated, Sieracki said.

Sieracki was able to demonstrate that the loss of activity was due to changes in pH and not a result of the temperature changes.

To find a buffer that would maintain a stable pH at varying temperatures, Sieracki first evaluated the behavior of several commonly used buffers over a range of temperatures. He saw that some buffers became more alkaline a
'/>"/>

Contact: Diana Yates
diya@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2

Related medicine news :

1. Climate change, gender differences, health among EurekAlert! 10 Most Popular Stories in 2007
2. APHA Adopts New Policies on Food Safety, Global Climate Change, Multidrug-Resistant Organisms, Breastfeeding and Feminine Hygiene
3. Summer Temperatures Linger, Extend Threat of Parasites
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New buffer resists pH change, even as temperature drops
(Date:1/22/2015)... 22, 2015 Yesterday, Senator Holly ... and Kristin Olsen (R-Modesto) were honored by the ... advancing biotechnology, biomedical science, medical device and pharmaceutical ... received the “2014 Life Sciences Champion” award from ...
(Date:1/22/2015)... 22, 2015 Four years since the release of ... coincided with the marriage of Avasa & Matthew Love – the ... is scheduled for release through White Swan Records on February 24, ... is a sacred path available to all of us to walk ...
(Date:1/22/2015)... New York, NY (PRWEB) January 22, 2015 ... component of overall health care gains momentum worldwide, Rev. ... York-based HealthCare Chaplaincy Network (HCCN), will be the ... advance spiritual care in Israel’s public health system. , The ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Lower-Auto-Insurance.com has released a ... kit in a car for reducing the costs of an ... will also be able to get lower prices for their vehicle ... an important role in determining policy costs. Because of this, ...
(Date:12/26/2014)... December 26, 2014 The US represents the ... report. In 2013, GlobalData’s forecast estimates that sales of branded ... market due to the high incidence of the disease, high ... 5EU, Japan, and China. Increased sales of CRC therapies over ...
Breaking Medicine News(10 mins):Health News:California Biomedical Community Honors Senator Holly Mitchell, Assemblymembers Susan Bonilla & Kristin Olsen 2Health News:California Biomedical Community Honors Senator Holly Mitchell, Assemblymembers Susan Bonilla & Kristin Olsen 3Health News:THE ROAD by Avasa & Matthew Love -- A New Album 2Health News:THE ROAD by Avasa & Matthew Love -- A New Album 3Health News:THE ROAD by Avasa & Matthew Love -- A New Album 4Health News:HealthCare Chaplaincy CEO to Keynote Spiritual Care Summit in Israel 2Health News:HealthCare Chaplaincy CEO to Keynote Spiritual Care Summit in Israel 3Health News:Driving Safe And Other Factors That Make Auto Insurance Affordable! 2Health News:US Colorectal Cancer Drug Market Analysis and Forecast to 2023 Report Available at ReportsnReports.com 2Health News:US Colorectal Cancer Drug Market Analysis and Forecast to 2023 Report Available at ReportsnReports.com 3Health News:US Colorectal Cancer Drug Market Analysis and Forecast to 2023 Report Available at ReportsnReports.com 4
... News) -- Standing water is a breeding ground for mosquitoes ... virus. Yet many urban families don,t take simple steps such ... say. Last summer, researchers polled 242 urban residents to ... and the risks posed by standing water, a mosquito breeding ...
... People with Parkinson,s disease who go to a neurologist for their ... placed in a nursing home and less likely to break a ... to a study published in the August 10, 2011, online issue ... of Neurology (AAN). The study also found that women ...
... , FRIDAY, Aug. 12 (HealthDay News) -- More doctors ... better survival rates, a new study finds. Researchers ... less-invasive laparoscopic surgical techniques, such as adjustable gastric banding, ... by morbidly obese patients. , , ...
... Aug. 12 (HealthDay News) -- Although being physically active is ... pain and improve their ability to get around, a new ... more sedentary than previously thought. Researchers from Northwestern University ... women and 40 percent of men with knee osteoarthritis are ...
... 2011Increasing public awareness of the childhood obesity epidemic may ... mass index (BMI), a measure of obesity in children, ... in Childhood Obesity , a peer-reviewed journal published ... online at http://www.liebertpub.com/chi . The HEALTHY Study ...
... of The Hawn Foundation, will present a lecture for ... a conference jointly sponsored by the New York Academy ... will speak about the MindUP curriculum, the Foundation,s signature ... social and emotional learning program designed for pre-kindergarten through ...
Cached Medicine News:Health News:Get Rid of Standing Water to Protect From Mosquitoes 2Health News:Study suggests seeing a neurologist helps people with Parkinson's live longer 2Health News:Weight-Loss Surgery Gains More Practitioners 2Health News:Many Arthritis Patients May Not Be Exercising Enough 2Health News:Positive impact of growing public awareness of obesity epidemic 2
(Date:1/23/2015)... and Markets ( http://www.researchandmarkets.com/research/vzhr6h/video ) has announced the ... Size, Share, Growth, Trends and Forecast 2014 - 2020" ... the topic of fascination since the era of the cold ... the Soviet Russia. Earlier even a small glimpse of human ...
(Date:1/23/2015)... , Jan. 23, 2015 Research and Markets ... the "Urinary Catheters Market (Indwelling or Foley Catheters, ... Global Industry Analysis, Size, Share, Growth, Trends and Forecast ... , At present, the global market for ...
(Date:1/23/2015)... , Jan. 23, 2015  A new analysis of ... D 2015 enrollment data shows that 81 percent of ... access and extra discounts at certain pharmacies. The findings ... "Preferred pharmacy plans are now the foundation of Medicare ...
Breaking Medicine Technology:Video Telemedicine Market- Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Video Telemedicine Market- Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3Urinary Catheters Market (Indwelling or Foley Catheters, Intermittent Catheters and Male External or Condom Catheters) - Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Urinary Catheters Market (Indwelling or Foley Catheters, Intermittent Catheters and Male External or Condom Catheters) - Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3New Analysis: 81 Percent of Medicare Part D Seniors Choose "Preferred Pharmacy" Plans in 2015 2
... Dehaier Medical Systems Ltd. (Nasdaq: ... in the development, assembly, marketing and sale of medical ... that it will release financial results for the second ... Monday, August 15, 2011 before the start of trading ...
... Pharmaceuticals, Inc. (Nasdaq: MAPP ) today announced financial results ... net loss for the three months ended June 30, 2011 was ... 2010. The net income for the first six months of 2011 ... during the same period in 2010.  Net income for the first ...
Cached Medicine Technology:Dehaier Medical to Report Second Quarter 2011 Financial Results on August 15 2MAP Pharmaceuticals Reports Second Quarter of 2011 Financial Results 2MAP Pharmaceuticals Reports Second Quarter of 2011 Financial Results 3MAP Pharmaceuticals Reports Second Quarter of 2011 Financial Results 4MAP Pharmaceuticals Reports Second Quarter of 2011 Financial Results 5MAP Pharmaceuticals Reports Second Quarter of 2011 Financial Results 6
... single-solution staining, Hema-Quik for Wright ... stain solution for five to ... water for five to fifteen ... by dipping quickly in deionized ...
... for staining of peripheral blood smears ... stain formulations still in use today. ... of Giemsa stain, although eosinophillic granules ... granules and nuclear chromatin stain a ...
This stain is the McNeal formula and can be substituted for Wright Stain solution....
... This stain is a ... a longer staining time but ... It is used in the ... and bone marrows. The results ...
Medicine Products: