Navigation Links
New biochip technology uses tiny whirlpools to corral microbes

WEST LAFAYETTE, Ind. - Researchers have demonstrated a new technology that combines a laser and electric fields to create tiny centrifuge-like whirlpools to separate particles and microbes by size, a potential lab-on-a-chip system for medicine and research.

The theory behind the technology, called rapid electrokinetic patterning - or REP - has been described in technical papers published between 2008 and 2011. Now the researchers have used the method for the first time to collect microscopic bacteria and fungi, said Steven T. Wereley, a Purdue University professor of mechanical engineering.

The technology could bring innovative sensors and analytical devices for lab-on-a-chip applications, or miniature instruments that perform measurements normally requiring large laboratory equipment. REP is a potential new tool for applications including medical diagnostics; testing food, water and contaminated soil; isolating DNA for gene sequencing; crime-scene forensics; and pharmaceutical manufacturing.

"The new results demonstrate that REP can be used to sort biological particles but also that the technique is a powerful tool for development of a high-performance on-chip bioassay system," Wereley said.

A research paper about the technology was featured on the cover of the Dec. 7 issue of Lab on a Chip magazine, and the work is highlighted as a news item in the Jan. 13 issue of Nature Photonics, posted online Dec. 27. Mechanical engineering doctoral student Jae-Sung Kwon, working extensively with Sandeep Ravindranath, a doctoral student in agricultural and biological engineering, was lead author of the Lab on a Chip paper.

The technology works by using a highly focused infrared laser to heat a fluid in a microchannel containing particles or bacteria. An electric field is applied, combining with the laser's heating action to circulate the fluid in a "microfluidic vortex," whirling mini-maelstroms one-tenth the width of a human hair that work like a centrifuge to isolate specific types of particles based on size.

Particles of different sizes can be isolated by changing the electrical frequency, and the vortex moves wherever the laser is pointed, representing a method for positioning specific types of particles for detection and analysis.

The Lab on a Chip paper was written by Kwon; Ravindranath; Aloke Kumar, a researcher at the Oak Ridge National Laboratory; Joseph Irudayaraj, a Purdue professor of agricultural and biological engineering and deputy director of the Bindley Bioscience Center; and Wereley.

Much of the research has been based at the Birck Nanotechnology Center at Purdue's Discovery Park, in collaboration with Irudayaraj's group in the Bindley Bioscience Center.

The researchers used REP to collect three types of microorganisms: a bacterium called Shewanella oneidensis MR-1; Saccharomyces cerevisiae, a single-cell spherical fungus; and Staphylococcus aureus, a spherical bacterium. The new findings demonstrate the tool's ability to perform size-based separation of microorganisms, Wereley said.

"By properly choosing the electrical frequency we can separate blood components, such as platelets," Wereley said. "Say you want to collect Shewanella bacteria, so you use a certain electrical frequency and collect them. Then the next day you want to collect platelets from blood. That's going to be a different frequency. We foresee the ability to dynamically select what you will collect, which you could not do that with conventional tools."

The overall research field is called "optoelectrical microfluidics." More research is needed before the technology is ready for commercialization.

"It won't be on the market in a year," Wereley said. "We are still in the research end of this. We are sort of at the stage of looking for the killer app for this technology."

REP may be used as a tool for nanomanufacturing because it shows promise for the assembly of suspended particles, called colloids. The ability to construct objects with colloids makes it possible to create structures with particular mechanical and thermal characteristics to manufacture electronic devices and tiny mechanical parts.

Purdue researchers are pursuing the technology for pharmaceutical manufacturing, Wereley said, because a number of drugs are manufactured from solid particles suspended in liquid. The particles have to be collected and separated from the liquid. This process is now done using filters and centrifuges.

REP also might be used to diagnose the presence of viruses, as well, although it has not yet been used to separate viruses from a sample, Wereley said.

Unlike conventional tools, REP requires only tiny samples, making it potentially practical for medical diagnostics and laboratory analysis.


Contact: Emil Venere
Purdue University

Related medicine news :

1. Biochip-based device for cell analysis
2. More changes in health care needed to fulfill promise of health information technology
3. Stem Cell Technology May Help Rejuvenate Immune Cells
4. Application Development Firm Anahata Technologies Pty Ltd Announces JavaFX 2.0 as Technology of Choice for Enterprise Applications
5. Technology Founders Commit to Combating U.S. Obesity
6. Atlanta Surgeon for LASIK Eye Surgery Publishes Study on Website About Car Technology to Monitor Drowsiness
7. InnoVision and The Danse Team Up to Tell the Story of Cutting Edge Technology Through 3D Animation
8. New technology may enable earlier cancer diagnosis
9. New technology allows scientists to capture and preserve cancer cells circulating in the bloodstream
10. GLOBODOX (Enterprise Document Management Software) Launches Contest for Legal Experts to Define Technology Challenges Expected in 2013
11. New Report on Water Treatment Equipment Market - by Technology, Application & Geography - Forecasting to 2017 will be Published by MarketsandMarkets
Post Your Comments:
Related Image:
New biochip technology uses tiny whirlpools to corral microbes
(Date:11/25/2015)... ... 25, 2015 , ... On November 10, 2015, Bohrer Brady, LLC filed a ... behalf of a home health care worker who provided companionship services for the elderly, ... workers employed by Humana, Inc., Humana at Home, Inc., and SeniorBridge Family Companies (CT), ...
(Date:11/25/2015)... ... November 25, 2015 , ... On November 25, 2015, ... for the Narconon network, announced the release of a new cutting edge recovery program ... organization has been working with drug- and alcohol-addicted individuals with the purpose to free ...
(Date:11/25/2015)... ... , ... SCOTTSDALE, AZ) - Today, Dr. Todd C. ... and non-surgical treatments, announced the expansion of his private practice capabilities with the ... Highly trained and nationally recognized for his natural approach, Dr. Todd Hobgood serves ...
(Date:11/25/2015)... ... November 25, 2015 , ... Today, Mothers Against Drunk ... roadways has dropped below 10,000 for the first time since 2011. In 2014, there ... According to data released by the National Highway Traffic Safety Administration (NHTSA), 32,675 people ...
(Date:11/25/2015)... ... November 25, 2015 , ... ... philanthropic seniors, is resulting in a way for homeless people to have a ... have launched a new initiative whereby they are repurposing plastic bags into sleeping ...
Breaking Medicine News(10 mins):
(Date:11/25/2015)... WASHINGTON , Nov. 25, 2015  The ... Obstetricians and Gynecologists (ACOG), and the March of ... bipartisan Protecting Our Infants Act of 2015 ... the number of newborns born exposed to drugs, ... Since the bill,s introduction, all three organizations have ...
(Date:11/25/2015)... AAIPharma Services Corp./Cambridge Major Laboratories, Inc. ... $15.8  Million to expand its laboratories and global ... The expansion will provide additional office space and ... of the pharmaceutical and biotechnology markets. ... up to 40,000 square feet of expanded development ...
(Date:11/25/2015)...  ARKRAY USA , Inc., a ... demonstrating the accuracy of its blood glucose meter systems. ... Diabetes and Cardiovascular Disease in Los Angeles ... ® 01 meter and the Assure ® ... to accurately measure glucose levels in blood is essential ...
Breaking Medicine Technology: