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New accurate diagnostic test for swine H1N1 influenza using RT-PCR technology
Date:6/9/2009

New Rochelle, NY, June 9, 2009A new, easy-to-perform method for detecting both seasonal influenza A virus and the emerging H1N1 swine-derived influenza A virus in human clinical samples offers a fast, sensitive, and cost-effective diagnostic test that runs on standard laboratory equipment. This timely and broadly applicable molecular technique is described in an article published online ahead of print in Vector-Borne and Zoonotic Diseases, a peer-reviewed journal published by Mary Ann Liebert, Inc. (www.liebertpub.com). The article is available free online at www.liebertpub.com/vbz

The recent emergence and global spread of a new swine flu virus highlights the urgent need for a reliable diagnostic test that can discriminate the H1N1 influenza virus from other strains and can be readily implemented in clinical testing laboratories. The molecular strategy described in the article in Vector-Borne and Zoonotic Diseases is based on proven and widely used Real-Time, Polymerase Chain Reaction (RT-PCR) technology. The authors of the report entitled "A Simple Method for Molecular Detection of Swine-Origin and Human-Origin Influenza A Virus" describe the development of a new molecular probe that improves on the existing PCR assay used to diagnose seasonal influenza and enables detection of both the seasonal and H1N1 influenza A viruses in the same patient sample using a simple test protocol. Laetitia Ninove and colleagues from Universit de la Mditerrane and Institut de Recherche pour le Dveloppement (Marseille, France), Hpitaux de Marseille, CEH Oxford (UK), and EHESP School of Public Health (Rennes, France) provide data to support the sensitivity and effectiveness of the SYBR Green RT-PCR one-step assay used for screening clinical samples to detect the presence of influenza A virus. In positive samples this is followed by the addition of two probes that are able to
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Contact: Vicki Cohn
vcohn@liebertpub.com
914-740-2156
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert

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