Navigation Links
New accurate diagnostic test for swine H1N1 influenza using RT-PCR technology
Date:6/9/2009

New Rochelle, NY, June 9, 2009A new, easy-to-perform method for detecting both seasonal influenza A virus and the emerging H1N1 swine-derived influenza A virus in human clinical samples offers a fast, sensitive, and cost-effective diagnostic test that runs on standard laboratory equipment. This timely and broadly applicable molecular technique is described in an article published online ahead of print in Vector-Borne and Zoonotic Diseases, a peer-reviewed journal published by Mary Ann Liebert, Inc. (www.liebertpub.com). The article is available free online at www.liebertpub.com/vbz

The recent emergence and global spread of a new swine flu virus highlights the urgent need for a reliable diagnostic test that can discriminate the H1N1 influenza virus from other strains and can be readily implemented in clinical testing laboratories. The molecular strategy described in the article in Vector-Borne and Zoonotic Diseases is based on proven and widely used Real-Time, Polymerase Chain Reaction (RT-PCR) technology. The authors of the report entitled "A Simple Method for Molecular Detection of Swine-Origin and Human-Origin Influenza A Virus" describe the development of a new molecular probe that improves on the existing PCR assay used to diagnose seasonal influenza and enables detection of both the seasonal and H1N1 influenza A viruses in the same patient sample using a simple test protocol. Laetitia Ninove and colleagues from Universit de la Mditerrane and Institut de Recherche pour le Dveloppement (Marseille, France), Hpitaux de Marseille, CEH Oxford (UK), and EHESP School of Public Health (Rennes, France) provide data to support the sensitivity and effectiveness of the SYBR Green RT-PCR one-step assay used for screening clinical samples to detect the presence of influenza A virus. In positive samples this is followed by the addition of two probes that are able to discriminate between the seasonal and swine H1N1 viruses to yield a definitive diagnosis.

Early, accurate identification of infected individuals will expedite appropriate antiviral therapy and enhance control and containment efforts. Furthermore, this new molecular test specifically amplifies and characterizes the viral genetic material, enabling rapid detection of new viral strains as they evolve. Using these genetic sequence data and making minor alterations to the PCR primers used in the assay, the test could be easily modified to detect newly emerging viral variants, including avian influenza strains.

"Early recognition of new influenza strains is vitally important for implementing effective control measures to limit spread. This cost-effective, comprehensive, and rapid test is a highly significant contribution to diagnostics that will greatly enhance our capacity to deal with future influenza outbreaks," says Stephen Higgs BSc, PhD, FRES, Editor-in-Chief of Vector-Borne and Zoonotic Diseases, and Associate Professor, Department of Pathology, Center for Biodefense & Emerging Infectious Diseases, Sealy Center for Vaccine Development and WHO Collaborating Center for Tropical Diseases, University of Texas Medical Branch, Galveston.


'/>"/>

Contact: Vicki Cohn
vcohn@liebertpub.com
914-740-2156
Mary Ann Liebert, Inc./Genetic Engineering News
Source:Eurekalert

Related medicine news :

1. MedSolutions: Accurate Diagnosis Is Critical to Reducing Healthcare Costs
2. Craneware Supports Hospitals Compliance and Upfront Collections with Accurate Estimates
3. Thumbs Up at ARVO for Diaton Tonometer - New Through Eyelid Glaucoma Test Clinical Study Presented, Results as Accurate as Gold Standard
4. Haverford Wellness Center Launches Web Site Devoted to Accurate Diagnosis and Proper Treatment to Cure Lyme Disease
5. CSI: Mayo Clinic Researchers Find Television Shows Inaccurately Portray Violent Crime
6. Media Urged to Correct Inaccurate References to Swine Flu
7. New MR sequence helps radiologists more accurately evaluate abnormalities of the uterus and ovaries
8. New womens imaging technique allows for a more accurate diagnosis of breast cancer
9. Non-invasive test accurately identifies gynecologic malignancies
10. RapidArc Radiotherapy Technology Enabling Fast, Accurate Treatments for Pennsylvania Cancer Patients
11. First noninvasive technique to accurately predict mutations in human brain tumors
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... ... 25, 2016 , ... As a lifelong Southern Californian, Dr. Omkar Marathe earned ... the David Geffen School of Medicine at UCLA. He trained in Internal Medicine at ... fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program where he had the opportunity ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... A recent ... that most people are unfamiliar with. The article goes on to state that individuals ... also many of these less common operations such as calf and cheek reduction. The ...
(Date:6/24/2016)... ... June 24, 2016 , ... The Pulmonary Hypertension Association ... it will receive two significant new grants to support its work to advance ... 25th anniversary by recognizing patients, medical professionals and scientists for their work in ...
(Date:6/24/2016)... ... June 24, 2016 , ... Topical BioMedics, Inc, makers of Topricin and MyPainAway Pain ... for a minimum wage raise to $12 an hour by 2020 and then adjusting it ... the lost value of the minimum wage, assure the wage floor does not erode again, ...
(Date:6/24/2016)... , ... June 24, 2016 , ... ... The Grove Investment Group (TGIG), has initiated cultivation and processing operations at its ... Vegas and Pahrump, Nevada. , Puradigm is the manufacturer of a complete system ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... Research and Markets has announced the addition of ... report to their offering. ... kidney failure, it replaces the function of kidneys by removing ... thus the treatment helps to keep the patient body,s electrolytes ... Increasing number of ESRD patients & substantial healthcare expenditure ...
(Date:6/23/2016)... Research and Markets has announced the addition of ... Forecast to 2022" report to their offering. ... date financial data derived from varied research sources to present ... impact on the market during the next five years, including ... sub markets, regional and country level analysis. The report provides ...
(Date:6/23/2016)... and INDIANAPOLIS , June 23, 2016 ... Lilly Diabetes Tomorrow,s Leaders Scholarship is any indication, the ... announced today online at www.diabetesscholars.org by the ... diabetes stand in the way of academic and community ... Foundation,s scholarship program since 2012, and continues to advocate ...
Breaking Medicine Technology: