Navigation Links
New DNA repair pathway
Date:11/9/2010

UC Davis researchers have found a new pathway for repairing DNA damaged by oxygen radicals. The results are published this week in the journal Proceedings of the National Academy of Sciences.

"This new inducible pathway gives cells greater capacity to repair oxidative damage," said Peter Beal, professor of chemistry at UC Davis and senior author of the paper.

As part of its inflammatory response, the body's immune system produces oxygen radicals, or reactive oxygen species, to kill bacteria, parasites or tumors. But chronic inflammation, for example in the gut, has been linked to cancer, said co-author Professor Sheila David, also of the Department of Chemistry.

Oxygen radicals are strongly linked to cancer and aging and are also formed during metabolism and upon exposure to environmental toxins and radiation. Understanding more about how this damage can be repaired could lead to a better understanding of the causes of some cancers.

Oxygen radicals can react with the four bases that make up the "letters" of DNA -- A, C, G and T -- so that the "spelling" of genes gets changed. The accumulation of spelling errors (called mutations) can lead to cancer.

David's laboratory studies an enzyme called NEIL1 that detects and repairs these aberrant or damaged bases before changes in the genome become permanent.

Beal's group works on RNA editing. The first step in turning a gene into a protein is to make a copy of the DNA in RNA. This messenger RNA is then translated into the chain of amino acids that makes up a protein. In some cases, this RNA is "edited" between the transcription from DNA and the translation into protein.

At a conference last year, Beal -- who happens to be David's husband -- spotted NEIL1 among a list of genes that had just been discovered to be subject to RNA editing, and passed the news on to David.

On investigation, they found that NEIL1's messenger RNA is edited by an enzyme called ADAR1. In that editing, one of the chains of amino acids that make up NEIL1 changes from lysine to arginine, causing a slight, but noticeable, change in the structure of the protein.

Using a cell line derived from nerve cells, the team found no editing of NEIL1 RNA in resting cells. But when the cells were treated with interferon, which is produced during inflammation and to fight off viruses, the cells started making ADAR1 and editing NEIL1.

"The interferon-treated cells had two forms of the NEIL1 protein, one with lysine and one with arginine," Beal said.

NEIL1 can fix a number of different damaged DNA bases that form when normal DNA bases are attacked by oxygen radicals. Beal and David found that the two different forms of NEIL1 had different abilities to act upon the damaged DNA bases: the basic, lysine version had a broader range but lower activity, while the edited, arginine form had higher activity but was effective against a more limited range of targets. That might give the cell more flexibility in responding to DNA damage.

Beal and David believe that the whole system works something like this: Inflammation creates oxygen radicals, which damage DNA, which is repaired by NEIL1. Inflammation also generates interferon, which induces ADAR1, which then edits NEIL1 to produce the more active, specific form to cope with more severe types of DNA base damage.


'/>"/>

Contact: Andy Fell
ahfell@ucdavis.edu
530-752-4533
University of California - Davis
Source:Eurekalert

Related medicine news :

1. Scientists Discover Molecular Pathway for Organ Tissue Regeneration and Repair
2. $250,000 grant awarded for groundbreaking ligament and tendon repair research
3. $250,000 Grant Awarded for Groundbreaking Ligament and Tendon Repair Research
4. Vitamin B3 May Help Repair Brain After a Stroke
5. Clitoraid Announces New Round of Clitoral Repair Surgeries in U.S. for FGM Victims
6. EPA's New Lead-Based Paint (LBP) Renovation, Repair and Painting Rule (RRP) Causes Headaches for Contractors and Property Owners Alike
7. Repair of torn knee meniscus at the time of ACL reconstruction is safe and effective for children
8. Minimally invasive sports hernia repair may get athletes back in the game faster, study says
9. Newly identified proteins critical to FA pathway DNA repair function
10. Stirling Dental Laboratory, Experts in Emergency Denture Repair Service, Gives Back to Community
11. Cedars-Sinai study examines regional use of minimally invasive repair of aneurysms
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/24/2016)... (PRWEB) , ... May 24, 2016 , ... ... to family wealth and to housing, with more advantaged communities providing richer opportunities. ... five types of interventions: (a) school improvement policies; (b) school choice policies; (c) ...
(Date:5/24/2016)... ... May 24, 2016 , ... Regenerative Medicine is being transformed by ongoing ... therapy technology, protocols and patient results as have been achieved with Okyanos Cell ... accessible standard of care for patients worldwide. , As the Medical Advisory Chairman at ...
(Date:5/24/2016)... ... , ... Backed by decades of experience, Metroloji Okulu works ... best suited solution to meet regulatory requirements. Their professional staff also performs on-site ... , Metroloji Okulu specializes in MadgeTech’s line of medical and pharmaceutical data ...
(Date:5/24/2016)... ... May 24, 2016 , ... ... Jonathan (Jon) Otterstatter to its board of directors. Otterstatter is co-founder, ... in the development of technological innovations that lead to broad-based healthcare solutions. , ...
(Date:5/24/2016)... ... , ... WaterAid launched the #perioddrama campaign to mark Menstrual Hygiene Day on May ... the world who do not have access to a toilet, even when they’re on their ... of #perioddrama. The (sometimes hilarious) results help shine a light on the awkwardness that women ...
Breaking Medicine News(10 mins):
(Date:5/24/2016)... LAWRENCE, Mass. , May 24, 2016  NxStage ... medical technology company focused on advancing renal care, today ... Officer, plans to participate in the following schedule of ... will be made available at http://ir.nxstage.com/ . ... Jefferies Healthcare Conference NY, NY           Friday, ...
(Date:5/24/2016)... HONG KONG , May 24, 2016 /PRNewswire/ ... primer stent de doble terapia del mundo, introduce ... fístula arteriovenosa. OrbusNeich, una compañía global ... cambian las vidas, ha expandido su cartera incluyendo ... catéteres balón JADE™ y Scoreflex™ PTA son los ...
(Date:5/24/2016)... Een app die artsen over ... patiënten kunnen behandelen, hun kennis kunnen delen en van ... nieuwe en revolutionaire MDLinking App, ontwikkeld door een internationale ... Hans Flu en oncologisch chirurg dr. Gijs van ... wordt op dinsdag 24 mei officieel gepresenteerd op het ...
Breaking Medicine Technology: