Navigation Links
Neuronal survival and axonal regrowth obtained in vitro
Date:7/24/2009

While repair of the central nervous system has long been considered impossible, French researchers from Inserm, the CNRS and the UPMC have just developed a strategy that could promote neuronal regeneration after injury. The in vitro studies have just been published in the journal PLoS ONE.

Repair of the central nervous system and restoration of voluntary motor activity through axonal re-growth has long been considered impossible in mammals. Over the last decade, numerous attempts proved disappointing overall. The Inserm team led by Alain Privat has recently shown that an essential component interfering with regeneration was due to the activity of astrocytes, feeder cells that surround neurons.

Normally, the primary role of astrocytes is to supply the nutrients necessary for neuronal function. In the event of spinal injury or lesion, astrocytes synthesize two particular proteins (glial fibrillary acidic protein (GFAP) and vimentin), which isolate the damaged neuron to prevent interference with the operation of the central nervous system.

While the protection is initially useful, in the long run it induces formation of impermeable cicatricial tissue around the neuron, thus constituting impenetrable scarring hostile to axonal regeneration and hence to propagation of nervous impulses. In the event of severe injury, the scarring engenders motor paralysis.

On the basis of the initial findings, the researchers pursued a strategy aimed at developing a therapeutic instrument to block formation of cicatricial tissue. In order to do so, they used gene therapy based on use of interfering RNA. The short RNA sequences, which were made to measure, were inserted into the cytoplasm of cultured astrocytes using a viral therapeutic vector. Once in the cell, the RNA activates mechanisms which block the synthesis of the two proteins secreted by astrocytes and responsible for cicatrix formation. Using that technique, the researchers succeeded in controlling the reaction of astrocytes and when the latter were cultured with neurons, they promoted neuronal survival and triggered axonal growth.

The promising results are now to be validated by in vivo studies. The next stage of the work, currently ongoing, applies the same method to the mouse. The approach may be used in the future in patients having undergone spinal injury.


'/>"/>

Contact: Alain Privat
privat@univ-montp2.fr
INSERM (Institut national de la sant et de la recherche mdicale)
Source:Eurekalert

Related medicine news :

1. St. Jude Scientists Discover a New Mechanism Controlling Neuronal Migration
2. Novel Photoconductive Cellular Depolarization Technology Reveals Insight Into Neuronal Growth, Guidance, and Regeneration
3. Neuronal regulators offer potential targets for cancer
4. Abbott Researchers Target Neuronal Nicotinic Receptors for Treatment of Pain and Cognition
5. Low level of neuronal receptor linked to mild cognitive impairment and Alzheimers disease
6. Nexavar in Combination with Chemotherapy Shown to Extend Progression-Free Survival in Patients with Advanced Breast Cancer
7. Genetic Variation Associated With Survival Advantage in African Americans With HIV
8. Study finds survival rates from gastrointestinal tumors improving among African-Americans
9. First Annual Congressional Womens Softball Game to Benefit the Young Survival Coalition
10. CPR Survival Rates for Older People Unchanged
11. LSUHSC research identifies enzyme that makes survival molecule for key vision cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... ... June 27, 2016 , ... "FCPX editors can now ... of Final Cut Pro X," said Christina Austin - CEO of Pixel Film Studios. ... Final Cut Pro X users can now reveal the media of their split ...
(Date:6/27/2016)... ... June 27, 2016 , ... A revolution is underway. ... ambulance transport experience for the millions of people who require these medical transport ... taxi industry through the use of technology. Now, SmartEMS has put forth an ...
(Date:6/26/2016)... ... , ... On June 10-11, 2016, A Forever Recovery, a holistic treatment center ... Breakfast Table in Battle Creek, MI, where the rehabilitation facility is located. This annual ... the world’s leading providers of cereal and other breakfast foods. Its residents often refer ...
(Date:6/26/2016)... (PRWEB) , ... June 26, 2016 , ... Brent Kasmer, a legally blind and certified ... be personalized through a fitness app. The fitness app plans to fix the two major ... only offer a one size fits all type program , They don’t eliminate ...
(Date:6/25/2016)... ... June 25, 2016 , ... Austin residents seeking Mohs surgery services, can now ... and to Dr. Russell Peckham for medical and surgical dermatology. , Dr. Dorsey brings ... The selective fellowship in Mohs Micrographic Surgery completed by Dr. Dorsey was under the ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... 24, 2016 Research and Markets has ... Companion Diagnostic Tests" report to their offering. ... Diagnostics The World Market for Companion Diagnostics ... diagnostics. Market analysis in the report includes the following: ... Vitro Diagnostic Kits) by Region (N. America, EU, ROW), 2015-2020 ...
(Date:6/24/2016)... , June 24, 2016 ... appointment of Dr. Edward Futcher to ... Director, effective June 23, 2016.Dr. Futcher was also ... and Governance Committees.  As a non-executive member of ... expertise and strategic counsel to VolitionRx in connection ...
(Date:6/23/2016)... 2016 Research and Markets has announced ... Forecast to 2022" report to their offering. ... the patients with kidney failure, it replaces the function of ... patient,s blood and thus the treatment helps to keep the ... balance. Increasing number of ESRD patients & ...
Breaking Medicine Technology: