Navigation Links
Neuronal survival and axonal regrowth obtained in vitro
Date:7/24/2009

While repair of the central nervous system has long been considered impossible, French researchers from Inserm, the CNRS and the UPMC have just developed a strategy that could promote neuronal regeneration after injury. The in vitro studies have just been published in the journal PLoS ONE.

Repair of the central nervous system and restoration of voluntary motor activity through axonal re-growth has long been considered impossible in mammals. Over the last decade, numerous attempts proved disappointing overall. The Inserm team led by Alain Privat has recently shown that an essential component interfering with regeneration was due to the activity of astrocytes, feeder cells that surround neurons.

Normally, the primary role of astrocytes is to supply the nutrients necessary for neuronal function. In the event of spinal injury or lesion, astrocytes synthesize two particular proteins (glial fibrillary acidic protein (GFAP) and vimentin), which isolate the damaged neuron to prevent interference with the operation of the central nervous system.

While the protection is initially useful, in the long run it induces formation of impermeable cicatricial tissue around the neuron, thus constituting impenetrable scarring hostile to axonal regeneration and hence to propagation of nervous impulses. In the event of severe injury, the scarring engenders motor paralysis.

On the basis of the initial findings, the researchers pursued a strategy aimed at developing a therapeutic instrument to block formation of cicatricial tissue. In order to do so, they used gene therapy based on use of interfering RNA. The short RNA sequences, which were made to measure, were inserted into the cytoplasm of cultured astrocytes using a viral therapeutic vector. Once in the cell, the RNA activates mechanisms which block the synthesis of the two proteins secreted by astrocytes and responsible for cicatrix formation. Using that technique, the researchers succeeded in controlling the reaction of astrocytes and when the latter were cultured with neurons, they promoted neuronal survival and triggered axonal growth.

The promising results are now to be validated by in vivo studies. The next stage of the work, currently ongoing, applies the same method to the mouse. The approach may be used in the future in patients having undergone spinal injury.


'/>"/>

Contact: Alain Privat
privat@univ-montp2.fr
INSERM (Institut national de la sant et de la recherche mdicale)
Source:Eurekalert

Related medicine news :

1. St. Jude Scientists Discover a New Mechanism Controlling Neuronal Migration
2. Novel Photoconductive Cellular Depolarization Technology Reveals Insight Into Neuronal Growth, Guidance, and Regeneration
3. Neuronal regulators offer potential targets for cancer
4. Abbott Researchers Target Neuronal Nicotinic Receptors for Treatment of Pain and Cognition
5. Low level of neuronal receptor linked to mild cognitive impairment and Alzheimers disease
6. Nexavar in Combination with Chemotherapy Shown to Extend Progression-Free Survival in Patients with Advanced Breast Cancer
7. Genetic Variation Associated With Survival Advantage in African Americans With HIV
8. Study finds survival rates from gastrointestinal tumors improving among African-Americans
9. First Annual Congressional Womens Softball Game to Benefit the Young Survival Coalition
10. CPR Survival Rates for Older People Unchanged
11. LSUHSC research identifies enzyme that makes survival molecule for key vision cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/27/2017)... ... February 27, 2017 , ... Much attention has been paid ... who become dependent on opioid painkillers has fallen short. From 1999 until 2010, fatal ... 237% increase in fatal overdoses in male populations.(1) , The proportion of women using ...
(Date:2/27/2017)... ... ... Elisa Guajardo Carothers is not your typical author. She went from working as a movie ... writes about God, when she isn’t swimming as a performing mermaid. , Her book isn’t ... Satan),” she offers a comedic look at the dysfunctions of God’s family, before Lucifer was ...
(Date:2/27/2017)... ... February 27, 2017 , ... This webinar , ... as a tool to characterize particle size distributions in the field of geoscience ... and novel scientific findings. It describes methods of optimized and standardized sample collection, ...
(Date:2/27/2017)... (PRWEB) , ... February 27, 2017 , ... POUGHKEEPSIE, N. ... ) has disclosed that despite scientific studies, the Center for Disease Control ( CDC ... Lyme disease . Kenneth B. Liegner, M.D. has compiled into a single volume ...
(Date:2/27/2017)... ... 2017 , ... Sunshine Coast Health Centre (SCHC), one of ... https://www.sunshinecoasthealthcentre.ca/ , is proud to announce the addition of a registered massage ... commitment to innovation in drug rehab and alcohol treatment strategies beyond a "punishment" ...
Breaking Medicine News(10 mins):
(Date:2/24/2017)... Following receiving CE Marking in ... s AMAR is a major milestone for the country where the ... ound care market in Israel ... for inclusion in the National Health Basket , Israel ... E-QURE Corp. (OTCQB: EQUR), a leader in medical devices for the treatment ...
(Date:2/24/2017)... and Markets has announced the addition of the "Dry eye Drugs ... ... Drugs Price Analysis and Strategies - 2016, provides drug pricing data and ... following questions: What are the key drugs ... in the Global Dry eye market? What are the ...
(Date:2/24/2017)... , Feb. 23, 2017 The ... of the PhenoTest BC Kit, performed on the ... identify organisms that cause bloodstream infections and provide ... to respond to (antibiotic sensitivity). The test also ... provide this important information, which can guide antibiotic ...
Breaking Medicine Technology: