Navigation Links
Neuronal survival and axonal regrowth obtained in vitro
Date:7/24/2009

While repair of the central nervous system has long been considered impossible, French researchers from Inserm, the CNRS and the UPMC have just developed a strategy that could promote neuronal regeneration after injury. The in vitro studies have just been published in the journal PLoS ONE.

Repair of the central nervous system and restoration of voluntary motor activity through axonal re-growth has long been considered impossible in mammals. Over the last decade, numerous attempts proved disappointing overall. The Inserm team led by Alain Privat has recently shown that an essential component interfering with regeneration was due to the activity of astrocytes, feeder cells that surround neurons.

Normally, the primary role of astrocytes is to supply the nutrients necessary for neuronal function. In the event of spinal injury or lesion, astrocytes synthesize two particular proteins (glial fibrillary acidic protein (GFAP) and vimentin), which isolate the damaged neuron to prevent interference with the operation of the central nervous system.

While the protection is initially useful, in the long run it induces formation of impermeable cicatricial tissue around the neuron, thus constituting impenetrable scarring hostile to axonal regeneration and hence to propagation of nervous impulses. In the event of severe injury, the scarring engenders motor paralysis.

On the basis of the initial findings, the researchers pursued a strategy aimed at developing a therapeutic instrument to block formation of cicatricial tissue. In order to do so, they used gene therapy based on use of interfering RNA. The short RNA sequences, which were made to measure, were inserted into the cytoplasm of cultured astrocytes using a viral therapeutic vector. Once in the cell, the RNA activates mechanisms which block the synthesis of the two proteins secreted by astrocytes and responsible for cicatrix formation. Using that technique, the researchers succeeded in controlling the reaction of astrocytes and when the latter were cultured with neurons, they promoted neuronal survival and triggered axonal growth.

The promising results are now to be validated by in vivo studies. The next stage of the work, currently ongoing, applies the same method to the mouse. The approach may be used in the future in patients having undergone spinal injury.


'/>"/>

Contact: Alain Privat
privat@univ-montp2.fr
INSERM (Institut national de la sant et de la recherche mdicale)
Source:Eurekalert

Related medicine news :

1. St. Jude Scientists Discover a New Mechanism Controlling Neuronal Migration
2. Novel Photoconductive Cellular Depolarization Technology Reveals Insight Into Neuronal Growth, Guidance, and Regeneration
3. Neuronal regulators offer potential targets for cancer
4. Abbott Researchers Target Neuronal Nicotinic Receptors for Treatment of Pain and Cognition
5. Low level of neuronal receptor linked to mild cognitive impairment and Alzheimers disease
6. Nexavar in Combination with Chemotherapy Shown to Extend Progression-Free Survival in Patients with Advanced Breast Cancer
7. Genetic Variation Associated With Survival Advantage in African Americans With HIV
8. Study finds survival rates from gastrointestinal tumors improving among African-Americans
9. First Annual Congressional Womens Softball Game to Benefit the Young Survival Coalition
10. CPR Survival Rates for Older People Unchanged
11. LSUHSC research identifies enzyme that makes survival molecule for key vision cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/11/2016)... ... , ... The Journal of Pain Research has seen a significant ... data taken from the Scopus database (Elsevier B.V.) and is a measure of a ... journal over a three year period and also the importance of the journals where ...
(Date:2/11/2016)... Petersburg, FL (PRWEB) , ... February 11, 2016 ... ... providers and advocates will discuss how to improve care by making data on ... pediatric heart disease. The Summit on Transparency and Public Reporting of Pediatric and ...
(Date:2/11/2016)... ... February 11, 2016 , ... The Commission for Case Manager ... of Commissioners. Individuals interested in volunteer board service are encouraged to apply. The ... settings and across allied health to contribute to its mission and vision. The ...
(Date:2/11/2016)... ... February 11, 2016 , ... Colorado spine surgeon, Donald Corenman, ... 334 spine surgeons to know in 2016 . The list consists of spine surgeons ... surgery. , Dr. Corenman understands the importance of clinical excellence; he has been ...
(Date:2/11/2016)... DC (PRWEB) , ... February 11, 2016 , ... The ... is based on evaluation of DataPoint’s team dedication and commitment to the ... companies comprising the annual list. The panel’s goal is to recognize and promote technology ...
Breaking Medicine News(10 mins):
(Date:2/11/2016)... YORK , Feb. 11, 2016 ... - Companion Diagnostics in Personalized Medicine and Cancer Therapy. ... - High-Growth Diagnostic Testing Markets. - Key ... Testing. - Molecular Diagnostics in Genetic Testing. ... Molecular Diagnostics Markets. - Over-the-Counter Diagnostic Products World ...
(Date:2/11/2016)... 11, 2016 The primary goal of this ... patterns on the usage of liquid biopsy. Key information ... - Timeframe of liquid biopsy adoption amidst future ... Evs—by organization type - Sample inflow to conduct liquid ... stool, serum, and so on. - Correlation analysis of ...
(Date:2/11/2016)... 2016 Stem cells are primitive cells found ... and the capacity to differentiate into mature cell types ... the first mouse embryonic stem cells were derived from ... that the first culturing of embryonic stem cells from ... produced until 2006 As a result of these discoveries, ...
Breaking Medicine Technology: