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Neuron connections seen in 3-D
Date:1/22/2010

the free movement of the vesicles, keeping them in their place until the electric impulse arrives, as well as determining the ease with which they will fuse with the membrane.

Sub-zero images

The technique upon which these discoveries are based, electron cryotomography, makes it possible to obtain three-dimensional images of the inside of cells and to minimise any changes to their structure. This is possible because the cells are not fixed with chemical reagents, but are vitrified in other words they are frozen so fast that the water inside them does not have time to crystallise, and remains in solid state.

These samples, which are always maintained at liquid nitrogen temperatures (below -140 C), can be viewed using specially-equipped microscopes. In addition, this method does not require any kind of additional staining, meaning the density of the biological structures can be observed directly.


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