Navigation Links
Neuron connections seen in 3-D
Date:1/22/2010

A team of researchers from the Max Planck Institute of Biochemistry, in Germany, led by the Spanish physicist Rubn Fernndez-Busnadiego, has managed to obtain 3D images of the vesicles and filaments involved in communication between neurons. The method is based on a novel technique in electron microscopy, which cools cells so quickly that their biological structures can be frozen while fully active.

"We used electron cryotomography, a new technique in microscopy based on ultra-fast freezing of cells, in order to study and obtain three-dimensional images of synapsis, the cellular structure in which the communication between neurons takes place in the brains of mammals" Rubn Fernndez-Busnadiego, lead author of the study which features on the front cover of this month's Journal of Cell Biology and a physicist at the Max Planck Institute of Biochemistry, in Germany, tells SINC.

During synapsis, a presynaptic cell (emitter) releases neurotransmitters to another post-synaptic one (recipient), generating an electric impulse in it, thereby allowing nervous information to be transmitted. During this study, the researchers focused on the tiny vesicles (measuring around 40 nanometres in diameter), which transport and release the neurotransmitters from the presynaptic terminals.

"Thanks to the use of certain pharmacological treatments and the advanced 3D imaging analysis method we have developed, it is possible to observe the huge range of filamentous structures that are within the presynaptic terminal and interact directly with the synaptic vesicles, as well as to learn about their crucial role in responding to the electrical activity of the brain," explains Fernndez-Busnadiego.

The filaments connect the vesicles and also connect them with the active area, the part of the cellular membrane from which the neurotransmitters are released. According to the Spanish physicist, these filamentous structures act as barriers that block the free movement of the vesicles, keeping them in their place until the electric impulse arrives, as well as determining the ease with which they will fuse with the membrane.

Sub-zero images

The technique upon which these discoveries are based, electron cryotomography, makes it possible to obtain three-dimensional images of the inside of cells and to minimise any changes to their structure. This is possible because the cells are not fixed with chemical reagents, but are vitrified in other words they are frozen so fast that the water inside them does not have time to crystallise, and remains in solid state.

These samples, which are always maintained at liquid nitrogen temperatures (below -140 C), can be viewed using specially-equipped microscopes. In addition, this method does not require any kind of additional staining, meaning the density of the biological structures can be observed directly.


'/>"/>

Contact: SINC
info@plataformasinc.es
34-914-251-820
FECYT - Spanish Foundation for Science and Technology
Source:Eurekalert  

Related medicine news :

1. Stem Cells Become Functioning Neurons in Mice
2. HAPPYneuron Launches the First Online Professional Destination for Clinical Cognitive Training.
3. HAPPYneuron releases iPhone Brain Game
4. HAPPYneuron Launches Beta Program for Innovative Cognitive Rehab Tools at AMIA Conference in San Francisco
5. 1 small step for neurons, 1 giant leap for nerve cell repair
6. Using synthetic evolution to study the brain: Researchers model key part of neurons
7. Famous Names Get Single Neurons Fired Up
8. Neuronal survival and axonal regrowth obtained in vitro
9. St. Jude Scientists Discover a New Mechanism Controlling Neuronal Migration
10. Imaging IDs Brain Neurons With Preference for Real Words
11. Zebrafish offer clues to treatments for motor neurone disease
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Neuron connections seen in 3-D
(Date:6/23/2017)... ... June 23, 2017 , ... 21 Middle East and ... has selected 21 leaders from government, business and civil society in 11 countries across ... U.S. this fall, engaging in a transformative exchange of knowledge and ideas with the ...
(Date:6/23/2017)... Jupiter, FL (PRWEB) , ... June 23, 2017 , ... ... the award winning series, which is slated to air fourth quarter 2017. American Farmer ... Christian D.A. Hansen, a Danish pharmacist, founded Chr. Hansen in Denmark in 1874 ...
(Date:6/23/2017)... ... June 23, 2017 , ... All-Star Insurance, a family managed agency that ... eastern Texas, is announcing the launch of a new charity drive to benefit women ... the United States reveal that an estimated 252, 710 new cases of invasive or ...
(Date:6/22/2017)... ... June 22, 2017 , ... Plastic Surgery Associates is proud to report that ... Each year, research and information firm, Castle Connolly, releases their list of the most ... marks the 3rd time that Dr. Canales has been recognized by Castle ...
(Date:6/22/2017)... ... 2017 , ... Despite its pervasiveness, many physicians are unfamiliar with how best ... with clinical practice. Now, however, a timely review has been published in the ... etiology of NeuP and educating preclinical scientists on its diagnosis and choice of treatment. ...
Breaking Medicine News(10 mins):
(Date:6/14/2017)... 14, 2017 The Bio Supply Management Alliance ... of Fremont and the Biomedical Manufacturing Network ... in California by providing a ... and fostering workforce development. The primary focus of this ... start-ups, as well as small and mid-sized biomedical companies. ...
(Date:6/12/2017)... SEATTLE , June 12, 2017 Kineta, Inc., ... today announced Kineta Vice President of R&D and ... at the Pandemic Preparedness for the Northwest and Beyond ... be held on June 14, 2017 from 8:30-10:30 AM PDT ... Dr. Bedard will be joined ...
(Date:6/9/2017)... 9, 2017 AirXpanders, Inc. (ASX: AXP) (AirXpanders ... design, manufacture, sale and distribution of the AeroForm® Tissue ... of its commercial roll-out in the United ... than one hundred (100) medical institutions and health systems, ... a needle-free alternative for women who choose reconstructive surgery ...
Breaking Medicine Technology: