Navigation Links
Neuroimaging: Live from inside the cell
Date:4/22/2014

This news release is available in German.

Reactive oxygen species are important intracellular signaling molecules, but their mode of action is complex: In low concentrations they regulate key aspects of cellular function and behavior, while at high concentrations they can cause "oxidative stress", which damages organelles, membranes and DNA. To analyze how redox signaling unfolds in single cells and organelles in real-time, an innovative optical microscopy technique has been developed jointly by the teams of LMU Professor Martin Kerschensteiner and TUM Professor Thomas Misgeld, both investigators of the Munich Cluster for Systems Neurology (SyNergy).

"Our new optical approach allows us to visualize the redox state of important cellular organelles, mitochondria, in real time in living tissue" Kerschensteiner says. Mitochondria are the cell's power plants, which convert nutrients into usable energy. In earlier studies, Kerschensteiner and Misgeld had obtained evidence that oxidative damage of mitochondria might contribute to the destruction of axons in inflammatory diseases such as multiple sclerosis.

The new method allows them to record the oxidation states of individual mitochondria with high spatial and temporal resolution. Kerschensteiner explains the motivation behind the development of the technique: "Redox signals have important physiological functions, but can also cause damage, for example when present in high concentrations around immune cells."

First surprises

Kerschensteiner and Misgeld used redox-sensitive variants of the Green Fluorescent Protein (GFP) as visualization tools. "By combining these with other biosensors and vital dyes, we were able to establish an approach that permits us to simultaneously monitor redox signals together with mitochondrial calcium currents, as well as changes in the electrical potential and the proton (pH) gradient across the mitochondrial membrane," says Thomas Misgeld.

The researchers have applied the technique to two experimental models, and have arrived at some unexpected insights. On the one hand, they have been able, for the first time, to study redox signal induction in response to neural damage in this case, spinal cord injury in the mammalian nervous system. The observations revealed that severance of an axon results in a wave of oxidation of the mitochondria, which begins at the site of damage and is propagated along the fiber. Furthermore, an influx of calcium at the site of axonal resection was shown to be essential for the ensuing functional damage to mitochondria.

Perhaps the most surprising outcome of the new study was that the study's first author, graduate student Michael Breckwoldt, was able to image, also for the first time, spontaneous contractions of mitochondria that are accompanied by a rapid shift in the redox state of the organelle. As Misgeld explains, "This appears to be a fail-safe system that is activated in response to stress and temporarily attenuates mitochondrial activity. Under pathological conditions, the contractions are more prolonged and may become irreversible, and this can ultimately result in irreparable damage to the nerve process."


'/>"/>
Contact: Vera Siegler
vera.siegler@tum.de
49-892-892-2731
Technische Universitaet Muenchen
Source:Eurekalert  

Related medicine news :

1. Corporate Whistle Blower Center Now Urges Medical Device Insiders To Be A Whistleblower If They Can Prove The Device Maker Knew Of A Failure & Said Nothing=Huge Rewards
2. Video shows the traffic inside a brain cell
3. Parents skin cancer concern doesnt keep kids inside
4. Smart scaffolding aims to rebuild tissue from the inside
5. New MRI technology to provide even better images of the inside of the human body
6. Corporate Whistle Blower Center Now Urges Medical Industry Insiders to Blow the Whistle if They Can Prove a Medical Product was Defective and Their Company Sold it Anyway
7. Skin Authority Creates One-of-a-Kind Solution for the One Billion People Worldwide Who are Vitamin D Deficient -- Groundbreaking Launch of "Inside Out" System
8. Prime Medical Associates’ Sleep Lab Featured in Insider Magazine
9. Corporate Whistle Blower Center Now Urges Physicians, Medical Device or Drug Insiders With Proof A Company Knowingly Sold A Bad Product to Become a Whistleblower
10. Corporate Whistle Blower Center Now Urges Physicians Medical Device and Drug Industry Insiders To Step Up-If They Have Proof Of Serious Wrongdoing or Overbilling
11. Fitness Celebrity and one of Canada’s Inside Fitness Hot and Fit Women of 2012, Nancy Di Nino, brings her Media Personality to host the Hot & Fit Release Party
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Neuroimaging: Live from inside the cell
(Date:5/6/2016)... ... May 06, 2016 , ... Featured ... provide a unique keynote address at the 2016 Learning Summit , June ... five community college students. Their stories—of complicated family issues, financial difficulties, and other ...
(Date:5/6/2016)... ... May 06, 2016 , ... Bluvault ( Bluvault Inc. ... offering for global clients of Coupa ( Coupa Software ) and other Cloud ... is a key component in the suite of innovative implementation services offered by ...
(Date:5/6/2016)... ... May 06, 2016 , ... Logically, spring weather, with ... when the weather is too cold, dry or hot, water on the eye surface ... absorb moisture from the surrounding air. There’s only one problem, according to radio show ...
(Date:5/6/2016)... ... May 06, 2016 , ... From the Speaker Podium to the Exhibit Floor ... its insights on managing Customers Engagement at SpeechTek 2016 Event, taking place May 23-25 ... deliver a Presentation on “5 Customer Engagement Strategies to improve Customer Satisfaction ...
(Date:5/6/2016)... ... May 06, 2016 , ... Expert ... . , Since launching in September, 2014 Sleepopolis has resided at Sleepopolis-mattress-reviews.com ... now include pillows, sheets, mattress toppers, bed frames, and more. , Founder and ...
Breaking Medicine News(10 mins):
(Date:5/5/2016)... , May 5, 2016 ... of the  "Europe Systemic Lupus Erythematosus Market ... to their offering.       (Logo: ... latest research Europe Systemic Lupus Erythematosus Market ... comprehensive insights into Systemic Lupus Erythematosus pipeline ...
(Date:5/4/2016)... 2016  It,s time for an upgrade. There are many medical recorders on the ... LCD, the illustrious DVMAXX HD  offers unparalleled connectivity and functionality.  Ampronix  is a ... class manufacturer of innovative technology.  Photo - http://photos.prnewswire.com/prnh/20160503/363416 ... ... ...
(Date:5/4/2016)... Yissum Research Development Company of the ... had signed an exclusive world-wide licensing and research agreement ... degradation and immunomodulatory drugs for cancer and immune dysfunction, ... first-in-class therapy for hematologic and solid malignancies. Financial terms ... novel technology was developed by Yinon Ben-Neriah , ...
Breaking Medicine Technology: