Navigation Links
Naturally fluorescent molecules may serve as cancer biomarker
Date:4/2/2009

Excess amounts of a naturally fluorescent molecule found in all living cells could serve as a natural biomarker for cancer, according to bioengineers.

NADH, or nicotinamide adenine dinucleotide, is a key coenzyme -- a non-protein molecule necessary for the functioning of an enzyme -- found mostly in the inner membrane of a cell's power plant, or mitochondria. It fuels a series of biochemical reactions that involve various enzymes to produce ATP, the major energy source in cells. In the event of disease or a metabolic disorder, these enzymes and their related reactions can become disabled, causing a buildup of unused NADH.

"Dysfunctional enzymes in the mitochondria are known to be associated with serious health problems such as cancer and neurodegenerative diseases," said Ahmed Heikal, associate professor of bioengineering, Penn State. "By detecting the level of NADH and its distribution inside living cells, we should be able to monitor the mitochondrial activity and thus the integrity of any given cell, without adding potentially toxic dyes or actually destroying the cell."

According to Heikal, one of the main challenges in cancer diagnosis is the ability to differentiate cancer cells from normal ones at the early stages of tumor progression.

To tease apart the critical difference between normal and cancerous cells, the researchers used the fluorescence of natural NADH. Using a combination of state-of-the-art spectroscopy and microscopy techniques, the researchers were able to convert such fluorescence into an accurate measure of NADH concentration in live cells. Heikal and Yu, graduate student, bioengineering, have found that the average concentration of NADH in breast cancer cells is about twice that in normal breast cells.

"If we are given two live cells, one normal and the other cancerous, we could differentiate between the two with confidence," said Heikal, whose team's findings appear today (April 2) in the Journal of Photochemistry and Photobiology B: Biology. "For the first time, we have been able to quantify the concentration of NADH in both live breast cells and breast cancer cells."

The researchers also looked at the amounts of NADH in the cell that is free and how much is bound to other enzymes. These amounts are different in normal and cancer cells.

"We realized that the fluorescence intensity not only depends upon the concentration of NADH but also on its structure -- free or enzyme-bound -- as well as its place inside the cell -- in the cytoplasm (non-nucleus part of the cell) or in mitochondria," explained Heikal. "Since a free NADH molecule would rotate -- tumble -- faster than enzyme-bound NADH, we were able to develop a technique called rotational diffusion imaging to establish a direct measure of the concentrations of free and enzyme-bound NADH throughout a living cell, whether in the cytosol (cell fluid) or the mitochondria."

To confirm their findings that disruption of chemical reactions that produce ATP can lead to an increase in NADH, Heikal and Yu exposed normal breast cells to potassium cyanide, a known inhibitor of some of these critical mitochondrial enzymes.

The researchers found that the NADH concentration in the normal cells increased when exposed to potassium cyanide. The relative amounts of NADH in the mitochondria also rose significantly.

Other researchers have previously measured the amount of NADH in cells using conventional biochemical techniques that require destroying the cells. However, Heikal believes measurements of dead cells provide no information about NADH distribution in the cells and may not be accurate or relevant for diagnostic or clinical use.

"The advantage of our non-destructive approach is that the NADH location in a cell relates to its function in cell survival," explained Heikal. "When you destroy the cell, you do not know where the NADH molecules existed inside the cell and what role they might have played in cell survival. For accurate diagnosis, you need to have the cellular context to better understand the problem."

According to the Penn State researcher, the ability to accurately measure NADH levels in a cell without killing it could have potential implications for related research on human health and drug delivery.

"Our technique is not limited to detecting cancer. Other neurodegenerative diseases related to mitochondrial anomalies can also be detected with our method," Heikal said. "We can also use our approach to quantify the efficiency of a new drug on manipulating the activities of mitochondrial enzymes associated with energy production in cells."


'/>"/>

Contact: Amitabh Avasthi
axa47@psu.edu
814-865-9481
Penn State
Source:Eurekalert

Related medicine news :

1. Childrens Bedding Naturally Enhances Emotional Well-being
2. Naturally Safe Zucol(TM) Brings Europes Leading Cold Remedy to United States
3. Naturally produced estrogen may protect women from Parkinsons disease
4. HPS Establishes Independent Website for its Patent-Pending Mood-Enhancing Compound Naturally Found in Sea Coral
5. HPS Files Comprehensive Patent Outside the Unites States for Mood-Enhancing Compound Naturally Found in Sea Coral
6. HPS Files Patent for Mood-Enhancing Compound Naturally Found in Sea Coral
7. Top Tips To Naturally Heal Dry Skin And Moisturize This Winter
8. Naturally Safe Cold Remedy Challenges Effectiveness of Over-The-Counter Cold Medications
9. Hain Celestial to Present at Canaccord Adams Healthy Living Naturally Boston Conference on October 15, 2008
10. New Skin Revitalizing Product Uses Naturally Occurring Serum for the Repair of Sun Damaged Skin
11. Children are naturally prone to be empathic and moral
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2016)... ... , ... A recent article published June 14 on E Online ... on to state that individuals are now more comfortable seeking to undergo not only ... calf and cheek reduction. The Los Angeles area medical group, Beverly Hills Physicians (BHP) ...
(Date:6/24/2016)... Fla. (PRWEB) , ... June 24, 2016 , ... Global ... Trend magazine’s 2016 Legal Elite. The attorneys chosen by their peers for this recognition ... Florida. , Seven Greenberg Traurig Shareholders received special honors as members of this year’s ...
(Date:6/24/2016)... ... June 24, 2016 , ... EB Medicine presented ... in Emergency Medicine conference in Ponte Vedra Beach, FL. The awards honor the ... Emergency Medicine Practice and Pediatric Emergency Medicine Practice. , “With this award, ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Venture ... Golf Classic Tournament held on June 20th at the Woodmont Country Club at ... Luke’s Wings, an organization dedicated to helping service members that have been wounded in ...
(Date:6/24/2016)... ... June 24, 2016 , ... Southern Illinois ... are co-chairs for the Illinois State Dental Society (ISDS) Foundation’s Mission of Mercy ... Gateway Convention Center in Collinsville. , They expect to treat approximately ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... 23, 2016  Guerbet announced today that it has ... Horizon Award . One of 12 suppliers ... for its support of Premier members through exceptional local ... and commitment to lower costs. ... of our outstanding customer service from Premier," says ...
(Date:6/23/2016)... -- The Biotechnology industry might still be ... opportunities to investors. Stock-Callers.com assesses the recent performances of ... XON ), Vertex Pharmaceuticals Inc. (NASDAQ: ... ), and Regeneron Pharmaceuticals Inc. (NASDAQ: REGN ... complimentary trade alerts at: http://stock-callers.com/registration ...
(Date:6/23/2016)... Research and Markets has announced the addition ... New Global Agenda " report to their offering. ... Europe - the 2016 edition is a ... identifies the driving factors coming into play which will influence ... and in the future. It provides a wealth of knowledge ...
Breaking Medicine Technology: